Preparation process for Penicillium oxalicum fermentation broth and application thereof in agriculture
A technology of Penicillium oxalate and a preparation process is applied in the fields of microorganisms and soil fertilizers, which can solve the problems of wheat freezing injury, hanging roots and dead seedlings, crop production damage, etc., and achieves the effects of improving physical and chemical properties, reducing preparation costs and promoting crop growth.
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Embodiment 1
[0025] Embodiment 1: The method for decomposing corn stalks in potted plants with HB1 fermented liquid:
[0026] The preparation of HB1 fermented liquid can adopt following method: Inoculate HB1 bacterial strain into the CMC-Na liquid culture medium of sterilization (CMC-Na 5 g, yeast extract 1.0 g, KH 2 PO 4 0.25 g, (NH 4 ) 2 SO 4 0.4 g, MgSO 4 ·7H 2 O 0.01 g, peptone 0.1 g, distilled water to 1000 mL, pH = 7), 30 ° C, 180 r / min, shaking culture for 24 h, to prepare HB1 bacterial suspension. Prepare corn stalk dry powder: beef extract peptone: HB1 bacterial suspension according to 10 g: 1 g: 1 mL, add 100 mL of water, mix well, cultivate at 30°C, set initial pH to 5, 180 r / min, and ferment for 72 h, preparing HB1 fermentation broth.
[0027] In a flowerpot with 1.5 kg of soil, 8.0 g of dry corn stalk powder was mixed evenly, then 1.5 mL of HB1 fermentation broth was sprayed on the soil surface, and the soil surface was watered to make the soil moisture content 25%...
Embodiment 2
[0028] Example 2 : The method of promoting the decomposition of corn stalks by HB1 fermentation liquid in the field of corn stalks returned to the field can be adopted:
[0029] After the corn is harvested, the corn stalks are routinely crushed, so that the crushed stalks are evenly spread on the soil surface, and the relative humidity of the soil is between 70% and 75% after watering. 2 Spray 50 kg of the HB1 fermented liquid prepared in Example 1, and immediately plow or rotary tillage to mix the crushed corn stalks with the plow layer soil. The corn stalk decomposition rate is 40% at 30 d, and 50 d The measured decomposition rate reached 75.5%.
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