Oxalic acid penicillium strain NJDL-03 and application thereof

A technology of NJDL-03 and Penicillium oxalicum, which is applied in the direction of fungi, organic fertilizers, microorganisms, etc., can solve the problems of wasting resources, polluting the environment, and high production costs, and achieves improved solubility, good application prospects, and high phosphorus-dissolving efficiency. Effect

Active Publication Date: 2016-02-03
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The expanded cultivation of microorganisms, conventional production mostly uses high-priced agricultural and sideline products such as soybean flour, bran, and yeast powder as raw materials, and the production cost is relatively high, which limits the further promotion and application of microbial fertilizers. 30% to 40% of the pro

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  • Oxalic acid penicillium strain NJDL-03 and application thereof
  • Oxalic acid penicillium strain NJDL-03 and application thereof
  • Oxalic acid penicillium strain NJDL-03 and application thereof

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Embodiment Construction

[0039] 1. Isolation and identification of strains

[0040] Collect healthy corn plant rhizosphere soil, in Ca 3 (PO 4 ) 2 To isolate phosphorus-dissolving fungi in selective culture as the only phosphorus source, the size of the phosphorus-dissolving circle is used as the initial screening index, and then the dissolved Ca is detected by liquid shake flask experiment. 3 (PO 4 ) 2 ability, found that the phosphate-dissolving fungal strain NJDL-03 can make Ca 3 (PO 4 ) 2 The concentration of soluble phosphorus in the solution reaches 1200mg / L. The biological identification and preservation of the strain NJDL-03 was carried out. It was identified that the strain NJDL-03 belonged to Penicillium oxalicum (Penicillium oxalicum), and it was preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on July 8, 2015. The deposit number is CGMCCNO.11061.

[0041] Phosphorus-dissolving fungus NJDL-03 belongs to Penicillium ox...

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Abstract

The invention discloses an oxalic acid penicillium strain NJDL-03 and application thereof. The strain NJDL-03 belongs to oxalic acid penicillium and is preserved in General Microorganism Center of China Committee for Culture Collection of Microorganisms on eighth, July, 2015, and the strain preservation number is CGMCC No. 11061. By means of the oxalic acid penicillium strain NJDL-03, solubility of hydroxyapatite can be remarkably improved, a large number of phosphate ions (PO<4><3->) are released out, hydroxyapatite lead [pb<10>(PO<4>)<6>(OH)<2>] which is quite prone to precipitating and fairly stable is generated through Pb<2+> in a precipitation environment, effectiveness of lead in the environment is lowered, and thereby the environment polluted by lead is restored. Oxalic acid penicillium fertilizer prepared through solid fermentation of the strain NJDL-03 is remarkable in application effects of improving solubility of hydroxylapatite, restoring lead pollution of soil, raising the utilization rate of calcareous soil phosphorus and promoting crop growth.

Description

technical field [0001] The invention belongs to the field of environmental biotechnology, and relates to a Penicillium oxalicum NJDL-03 bacterial strain and its application. The Penicillium oxalicum NJDL-03 bacterial strain is used to improve the solubility of hydroxyapatite and repair lead-polluted water bodies and soils. The NJDL-03 is used to improve the solubility of hydroxyapatite. Penicillium oxalicum phosphate-solubilizing solid bacterial fertilizer prepared by the strain improves phosphorus utilization efficiency in calcareous soil and remediates lead-contaminated soil. Background technique: [0002] The unrestricted exploitation and use of various natural mineral resources by human beings and the arbitrary discharge of heavy metal (lead)-containing waste residues, waste water, and waste gas have caused heavy metals (lead) to enter the environment in various forms, especially the soil on both sides of the road due to lead vehicle exhaust. Subsidence, the soil around ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C05F11/08C12R1/80
Inventor 王世梅李真戴乐天韦中徐阳春沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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