Phosphorus-solubilizing fungi and application thereof to preparation of biological fungus fertilizer
A technology for dephosphorizing fungi and microbial strains, applied in the directions of microorganism-based methods, microorganisms, fungi, etc., can solve the problems of low profit margin, discount on market competitiveness, increase production cost of biological bacterial fertilizer, etc. good fertilizer effect
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[0012] 1. Cultivation of Mo-Po
[0013] Mo-Po strain test tube slant seed culture: inoculate the thallus of this strain into PDA medium (200g of peeled potatoes are chopped, boiled in water for 30 minutes and then filtered to get the filtrate, 20g of sucrose, 20g of agar, replenish water to 1000ml, natural pH) On the slant, cultivate at 15-30° C. for 3-10 days to obtain slant seeds, and the suitable culture condition is to cultivate at 28° C. for 6 days.
[0014] The cultivation of Mo-Po bacterial strain liquid seed: the MC substratum (molasses 30g, cornflour 5g, ammonium sulfate 5g, sodium chloride 6g, potassium dihydrogen sulfate 0.5g, tricalcium phosphate 5g, the inclined plane seed inoculated into the MC medium in the triangular flask, 1000ml of water, pH 6.5-7.0.), 15-30°C, 50-350rpm, shaker culture for 3-7 days. The optimum culture conditions are 28°C, 200rpm culture 4.
[0015] Mass cultivation of Mo-Po bacterial strain fermenter: the liquid seed is inserted in the MC...
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