Phosphorus-solubilizing fungi and application thereof to preparation of biological fungus fertilizer

A technology for dephosphorizing fungi and microbial strains, applied in the directions of microorganism-based methods, microorganisms, fungi, etc., can solve the problems of low profit margin, discount on market competitiveness, increase production cost of biological bacterial fertilizer, etc. good fertilizer effect

Inactive Publication Date: 2011-09-07
YUNNAN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

Organic fertilizer is a product with a very low profit margin. This method undoubtedly increases the

Method used

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Embodiment Construction

[0012] 1. Cultivation of Mo-Po

[0013] Mo-Po strain test tube slant seed culture: inoculate the thallus of this strain into PDA medium (200g of peeled potatoes are chopped, boiled in water for 30 minutes and then filtered to get the filtrate, 20g of sucrose, 20g of agar, replenish water to 1000ml, natural pH) On the slant, cultivate at 15-30° C. for 3-10 days to obtain slant seeds, and the suitable culture condition is to cultivate at 28° C. for 6 days.

[0014] The cultivation of Mo-Po bacterial strain liquid seed: the MC substratum (molasses 30g, cornflour 5g, ammonium sulfate 5g, sodium chloride 6g, potassium dihydrogen sulfate 0.5g, tricalcium phosphate 5g, the inclined plane seed inoculated into the MC medium in the triangular flask, 1000ml of water, pH 6.5-7.0.), 15-30°C, 50-350rpm, shaker culture for 3-7 days. The optimum culture conditions are 28°C, 200rpm culture 4.

[0015] Mass cultivation of Mo-Po bacterial strain fermenter: the liquid seed is inserted in the MC...

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Abstract

The invention relates to phosphorus-solubilizing fungi and application thereof to preparation of a biological fungus fertilizer, belonging to the technical field of microbial fertilizers. The microorganism in the invention is a penicillium oxalicum Mo-Po strain which is preserved at the China General Microbiological Culture Collection Center on September 25th, 2010 with a collection number of CGMCC No. 4209. An ITS4 gene sequence of the Mo-Po strain is a main characteristic basis for identifying the strain, wherein the sequence number of the sequence is HQ703581 in a Gene Bank database. Tobacco waste can be thoroughly decomposed and converted into the biological fungus fertilizer by the microorganism with a composting fermentation method. The prepared microorganism has a good effect of promoting plant growth and can be used for substituting a chemical phosphorus fertilizer. The prepared biological fungus fertilizer has the characteristics of low cost, high fertilizer efficiency, no residue and safety to people and livestock and is a high-efficiency biological fungus fertilizer in organic agricultural planting.

Description

Technical field: [0001] The invention relates to a phosphorus-dissolving microorganism and its application in preparing biological bacterial fertilizer, which belongs to the technical field of microbial bacterial fertilizer. Background technique: [0002] Phosphorus is an indispensable and important element for plant growth, but the phosphorus content in the soil that can be absorbed and utilized by plants is very low, generally only accounting for 2-3% of the total phosphorus. In order to meet the nutritional needs of crop growth, a large amount of chemical phosphorus fertilizers need to be applied to agricultural land every year. Only 10-25% of the chemical phosphorus fertilizer applied is absorbed and utilized by plants, and the rest is related to the Ca in the soil. 2+ , Fe 3+ , Al 3+ Such metal cations combine to form insoluble phosphate, or are adsorbed by calcium carbonate, aluminum oxide, iron oxide, and aluminum silicate in the soil, thereby increasing the conten...

Claims

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Application Information

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IPC IPC(8): C12N1/14C05F11/08C12R1/80
Inventor 莫明和陈明会曹永红周峰黄英
Owner YUNNAN UNIV
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