Penicillium oxalicum, preparation method and application thereof
A technology of Penicillium oxalate and nucleotide sequence, which is applied in the field of biocontrol strains, can solve the problems of no camellia canker disease, less Penicillium, etc., achieves stable ecological environment and biocontrol effect, reduces control cost, and has a wide antibacterial spectrum. Effect
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Embodiment 1
[0041] The separation, screening and identification of Penicilium oxalicum strain F217-3 are as follows:
[0042] A. Separation and purification of antagonistic fungi: Take 10g of healthy Camellia oleifera rhizosphere soil sample and add it to a triangular flask filled with 90mL sterile water and 30-50 glass beads to make a suspension with a concentration of 1:10. After shaking in a shaker at 180rpm for 30min, the bacterial suspension was placed in a water bath at 80°C for 15min. Transfer 1 mL of the soil suspension into a test tube filled with 9 mL of sterile water to make 10 -1 Bacterial suspension, and so on, get 10 -2 , 10 -3 , 10 -4 , 10 -5 and 10 -6 bacterial suspension. After serial dilution, pipette 100 μL for a dilution factor of 10 -3 -10 -6The bacterial suspension was evenly spread on the separation medium, and the medium formula was as follows (1L): Weigh 200g of potatoes, peel them, cut into small pieces and boil for 30min, add 20g of glucose after filteri...
Embodiment 2
[0050] Example 2: Study on the biological characteristics of Penicilium oxalicum strain F217-3
[0051] A. The best medium formula:
[0052] Along the edge of the antagonistic bacteria colonies cultured on the PSA medium for 5 days, the bacterial cakes (7 mm in diameter) with the same age were collected, inoculated in the center of the five test medium plates, and cultured at 28°C. After culturing for 6 days, measure the diameter of the colony by the cross method or estimate the percentage of the area of the colony to the area of the entire plate by visual inspection, 5 plates for each treatment, and the experiment was repeated 3 times (the same below).
[0053] The colony growth of Penicillium oxalicum on different media is as shown in Table 1. The results show that this Penicillium grows the best on Chase's medium (CA), and the colony is the largest, followed by PSA medium, and in clear water agar medium. does not grow.
[0054] The influence of table 1 different media...
Embodiment 3
[0084] Plate confrontation experiment between Penicilium oxalicum strain F217-3 and various pathogens of camellia oleifera.
[0085]The pathogenic bacteria cake and the F217-3 bacteria cake with a diameter of 7mm were respectively laid out. The pathogenic bacteria cake was placed in the center of the plate, and the antagonistic bacteria F217-3 bacteria cake was placed equidistantly (1.5cm from the center of the plate) on both sides of the center, forming a straight line with the pathogenic bacteria. Place them in a constant temperature incubator at 28°C for culture, observe the presence and size of the zone of inhibition after 5 days, and repeat each treatment 3 times. The results are shown in Table 1. The results showed that the antagonism of Bacillus amyloliquefaciens D2WM on camellia oleifera anthracnose, leaf spot, soft rot, black spot and canker was more than 90%, especially effective on camellia anthracnose, canker and black spot.
[0086] Table 6 The bacteriostatic rat...
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