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Preparation method of enteric probiotic and prebiotic compound preparation

A technology of dissolving probiotics and compound preparations, applied in the direction of microorganism-based methods, biochemical equipment and methods, and bacteria used in food preparation, can solve the problem of low number of viable bacteria, achieve high embedding rate and excellent preparation process Simple, shelf-life-extending effects

Pending Publication Date: 2022-02-08
山东晶辉生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ordinary probiotic preparations are affected by high temperature and gastric juice during oral administration, and the number of viable bacteria that actually reach the intestinal tract is very small

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: A kind of preparation method of enteric-coated probiotics and prebiotics compound preparation

[0031] Specifically include the following steps:

[0032] (1) Activation and fermentation of probiotic strains:

[0033] Bifidobacterium longum, Lactobacillus rhamnosus, and Lactobacillus reuteri were activated in the culture medium, and then inoculated into the culture medium according to the inoculation amount of 0.8%, fermented at 37°C for 14 hours, and the pH was controlled at 5.5-6.0; Low-temperature high-speed centrifugation at 4°C and 10,000 rpm, discard the supernatant to obtain bacteria;

[0034] (2) Preparation method of probiotic freeze-dried powder:

[0035] The cells were washed with buffer solution for 2-3 times, then resuspended with twice the weight of dextrin and skimmed milk powder mixed preparation, and the resuspension was freeze-dried for 12 hours to obtain a freeze-dried product, which was ground for 200 Mesh sieve to get probiotic compo...

Embodiment 2

[0047] Embodiment 2: A kind of preparation method of enteric-coated probiotics and prebiotics compound preparation

[0048] Specifically include the following steps:

[0049] (1) Activate Lactobacillus casei, Lactobacillus rhamnosus, and Lactobacillus plantarum in the culture medium, then inoculate them into the culture medium according to the inoculation amount of 0.4%, ferment at 37°C for 16 hours, and control the pH at 5.5-6.4; 4°C, low-temperature high-speed centrifugation at 10,000 rpm, discard the supernatant, and obtain the bacteria;

[0050] (2) The cells were washed with buffer solution for 2-3 times, then resuspended with a mixture of dextrin and skim milk powder twice the weight, and the resuspension was freeze-dried for 12 hours to obtain a freeze-dried product, which was then subjected to Grind and pass through a 200-mesh sieve to obtain probiotic compound powder;

[0051] (3) Suspend 100 g of probiotic compound powder in 900 g of vegetable oil, suspend at 800 r...

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PUM

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Abstract

The invention relates to the field of probiotics and prebiotic preparations, and particularly discloses a preparation method of an enteric probiotic and prebiotic compound preparation. The probiotics are embedded through edible materials, so that a final preparation is resistant to acid and high temperature and convenient to store and use, and people do not need to worry about inactivation of the high-temperature probiotics. The probiotics are subjected to a vacuum freeze-drying process, the moisture content is extremely low, the probiotics are suspended with edible oil, the shelf life of the product is greatly prolonged, a special transportation device is not used in the sales and transportation process of the product, the transportation cost is reduced, the product price is more popular, and the sales space is increased. The preparation process of the enteric probiotics and prebiotics is relatively simple, the embedding rate is high, the processing cost is low, and the preparation method is suitable for industrial production.

Description

technical field [0001] The invention relates to the field of probiotics and prebiotics preparations, in particular to a preparation method for a compound preparation of enteric-coated probiotics and prebiotics. Background technique [0002] In the 21st century, with the progress and development of biotechnology, the metabolism and function of various bioactive functional ingredients in the body have been further revealed. Consumers are increasingly pursuing specific functions in food due to the continuous improvement of health awareness. more intense. As of 2019, the global probiotic supplement industry has reached US$6.1 billion, while China's industry has reached RMB 4.24 billion, a year-on-year increase of 18%. The probiotics market has maintained a growth rate of 15% to 18% in recent years, far higher than the global average growth rate of 5%. Under the trend of consumption upgrading, the domestic probiotics product market has gradually entered a mature stage of "high g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135A23L33/21A23P10/30A23L3/3499C12N1/20C12R1/225C12R1/25C12R1/23C12R1/01
CPCA23L33/135A23L33/21A23P10/30A23L3/3499C12N1/20A23V2002/00A23V2400/173A23V2400/125A23V2400/175A23V2400/533A23V2400/169A23V2250/5026A23V2250/5432A23V2250/51088A23V2250/6402A23V2250/5114A23V2250/28
Inventor 杨林青周昕琪于海勤孔娟桑贺杨林慧高伟霞朱瑞清初敬珠李喜英曹利霞鄢雁
Owner 山东晶辉生物技术有限公司
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