Preparation method of enteric probiotic and prebiotic compound preparation
A technology of dissolving probiotics and compound preparations, applied in the direction of microorganism-based methods, biochemical equipment and methods, and bacteria used in food preparation, can solve the problem of low number of viable bacteria, achieve high embedding rate and excellent preparation process Simple, shelf-life-extending effects
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Embodiment 1
[0030] Embodiment 1: A kind of preparation method of enteric-coated probiotics and prebiotics compound preparation
[0031] Specifically include the following steps:
[0032] (1) Activation and fermentation of probiotic strains:
[0033] Bifidobacterium longum, Lactobacillus rhamnosus, and Lactobacillus reuteri were activated in the culture medium, and then inoculated into the culture medium according to the inoculation amount of 0.8%, fermented at 37°C for 14 hours, and the pH was controlled at 5.5-6.0; Low-temperature high-speed centrifugation at 4°C and 10,000 rpm, discard the supernatant to obtain bacteria;
[0034] (2) Preparation method of probiotic freeze-dried powder:
[0035] The cells were washed with buffer solution for 2-3 times, then resuspended with twice the weight of dextrin and skimmed milk powder mixed preparation, and the resuspension was freeze-dried for 12 hours to obtain a freeze-dried product, which was ground for 200 Mesh sieve to get probiotic compo...
Embodiment 2
[0047] Embodiment 2: A kind of preparation method of enteric-coated probiotics and prebiotics compound preparation
[0048] Specifically include the following steps:
[0049] (1) Activate Lactobacillus casei, Lactobacillus rhamnosus, and Lactobacillus plantarum in the culture medium, then inoculate them into the culture medium according to the inoculation amount of 0.4%, ferment at 37°C for 16 hours, and control the pH at 5.5-6.4; 4°C, low-temperature high-speed centrifugation at 10,000 rpm, discard the supernatant, and obtain the bacteria;
[0050] (2) The cells were washed with buffer solution for 2-3 times, then resuspended with a mixture of dextrin and skim milk powder twice the weight, and the resuspension was freeze-dried for 12 hours to obtain a freeze-dried product, which was then subjected to Grind and pass through a 200-mesh sieve to obtain probiotic compound powder;
[0051] (3) Suspend 100 g of probiotic compound powder in 900 g of vegetable oil, suspend at 800 r...
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