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Agricultural straw degrading fungus penicillium oxalicum NJGZ-2 and fungal agent thereof

A NJGZ-2, Penicillium oxalate technology, applied in fungi, organic fertilizers, microorganisms, etc., can solve the problems of high treatment cost and secondary pollution

Inactive Publication Date: 2014-03-26
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Straw resource treatment includes physical, chemical and biological methods, but the first two methods are costly and easy to cause secondary pollution. Therefore, microbial treatment is mostly used at present.

Method used

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  • Agricultural straw degrading fungus penicillium oxalicum NJGZ-2 and fungal agent thereof
  • Agricultural straw degrading fungus penicillium oxalicum NJGZ-2 and fungal agent thereof
  • Agricultural straw degrading fungus penicillium oxalicum NJGZ-2 and fungal agent thereof

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Embodiment Construction

[0026] 1) Isolation of efficient straw-degrading strains: The degrading bacteria were screened from the soil of rotting leaves in Zijin Mountain, Nanjing. The suspension of the soil samples diluted step by step was applied to the CMC-Congo red plate by the gradient dilution coating method, and cultured at 30°C to screen the cellulose-degrading bacteria. The formula of the above-mentioned CMC-Congo red medium is: sodium carboxymethylcellulose (CMC-Na) 10g, (NH 4 ) 2 SO 4 2g, KH 2 PO 4 1g, MgSO 4 ·7H 2O0.5g, Congo red 0.2g, agar 20g, water 1000mL, autoclave at 121℃ for 20min.

[0027] Purify and isolate the strains that produce larger transparent circles and grow faster on the CMC-Congo red plate, and then inoculate them on the rice straw powder medium with rice straw as the only carbon source for culture, and compare the growth speed and Finally, the high-temperature fungus NJGZ-2, which can efficiently degrade tobacco stalks, was obtained. On the PDA plate, the center ...

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Abstract

The invention relates to a normal-temperature fungus NJGZ-2 capable of degrading agricultural waste straw, belonging to the agricultural waste resource utilization technology. The class of the strain NJGZ-2 is named penicillium oxalicum, and the culture is collected in the China General Microbiological Culture Collection Center (CGMCC), with collection number CGMCC No.7527. Tests indicate that after the rice straw is inoculated with a spore suspension (1% 10<7> spores / mL), the weight loss ratio of the rice straw is 59.7% after 7 days of 170r / min liquid fermentation culture at 30 DEG C. In the fermentation crude enzyme extraction liquid, the activities of incision enzyme, beta-glucosidase, xylanase, beta-xylosidase and total cellulose are 113.3U / gds, 11.6U / gds, 1,439.7U / gds, 2.0U / gds and 13.8U / gds respectively. The crude enzyme liquid can hydrolyze low-concentration alkali-treated corncob to generate high-concentration reducing sugar (962.2mg of reducing sugar / gram of substrate). According to the invention, the agricultural waste straw is degraded by the microbial fermentation technology and is converted into organic fertilizer, therefore, the waste can be turned into wealth to protect the environment, and application prospects are broad.

Description

1. Technical field [0001] The invention relates to an agricultural stalk-degrading fungus and its bacterial agent, which belongs to the intensive agricultural production technology and is specially used for degrading waste stalks to produce organic fertilizers and realizing the resource utilization of organic wastes. 2. Background technology [0002] my country is a large agricultural country. How to deal with crop straws is a problem faced by most agricultural areas. If a large amount of waste straws are not disposed of in time, it will not only affect normal farming, but also become a place for breeding of diseases and insect pests; if it is burned, it will not only seriously It pollutes the environment and also wastes the useful resources and energy contained in the straw. Using straw-degrading bacteria to treat waste straw has the characteristics of environmental protection, energy saving and high efficiency, and meets the requirements of sustainable development. Agricul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C05F11/08C12R1/80
Inventor 徐阳春廖汉鹏韦中沈其荣
Owner NANJING AGRICULTURAL UNIVERSITY
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