43 results about "Beta-Xylosidase" patented technology

Xylose-containing plant material may be hydrolyzed to xylose using a β-D-xylosidase which exhibits unexpectedly high activity. The enzyme has a kcat value for catalysis of approximately 185 sec−1 for 1,4-β-D-xylobiose (X2) when measured at a pH of 5.3 and a temperature of 25° C.; this is at least 10-fold greater than reported for other xylosidases at 25° C. and their optimal pH. The enzyme also has an isoelectric point of approximately 4.4. When reacted at a pH between about 4.5 and about 7.7, the β-D-xylosidase exhibits surprisingly high activity for hydrolyzing xylose-containing plant materials to xylose. The xylose released from plant materials may then be converted to other secondary products such as ethanol by fermentation or other reaction. This β-D-xylosidase may be used alone or in combination with other hydrolytic or xylanolytic enzymes for treatment of lignocellulosic or hemicellulosic plant materials or plant material hydrolysates or xylooligosaccharides.

The present disclosure is directed, in a first aspect, to the use of inverting beta-xylosidase enzymes to reduce byproduct formation and increase the yield of fermentation products, as well as, in a second aspect, to the use of retaining beta-xylosidase enzymes to improve production of alkyl-beta-xylopyranoside compounds, in a simultaneous saccharification and fermentation reactions.

The invention discloses a making method of heat-proof xylanase, heat-proof beta-xylosidase or heat-proof beta-glucosidase, which comprises the following steps: adopting agricultural waste as carbon source; fermenting Paecilomyces thermophila J18 at 40-60 deg.c to obtain the ferment liquid with heat-proof xylanase, heat-proof beta-xylosidase or heat-proof beta-glucosidase; purifying; obtaining electrophoretic grade product.

A novel glycosyl hydrolase with activities of beta-xylosidase and beta-glucosidase is provided. Said glycosyl hydrolase can convert 7-xylosyltaxane compounds to 7-hydroxyltaxane compounds.

The present invention provides process for treating crop kernels, comprising the steps of a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of a beta-xylosidase, wherein step c) is performed before, during or after step b).

The invention discloses a comprehensive utilization method of oil-tea camellia shells. The method comprises the following steps: crushing oil-tea camellia shells, performing alkaline pretreatment, and performing solid-liquid separation to obtain black liquor and solid residues; performing enzymolysis on the solid residues through cellulase to prepare monosaccharide and performing alcohol fermentation, and preparing ethanol; regulating pH of the black liquor, and performing solid-liquid separation to obtain residues and a vanillic aldehyde solution; and performing enzymolysis on the residues through xylanase to prepare an xylooligosaccharide solution and residues, and oxidizing the residues to prepare the vanillic aldehyde. According to the comprehensive utilization method, the solid residues obtained by solid-liquid separation are hydrolyzed by cellulase and further fermented to prepare fuel ethanol by using the condition that the oil-tea camellia shells are pretreated by sodium hydroxide; the black liquor obtained by solid-liquid separation is neutralized to the pH of 6, wherein the supernatant obtained by solid-liquid separation contains high-concentration vanillic aldehyde (lignin oxidization is not required), enzyme hydrolysis is carried out on the precipitation to prepare xylooligosaccharide by employing xylanase containing a small amount of beta-xylosidase, and further the residues obtained by enzymolysis are further oxidized to prepare the vanillic aldehyde. Therefore, according to research of the process, the biomass can be totally utilized for preparing the fuel ethanol, xylooligosaccharide and vanillic aldehyde finally.

The present invention relates to a Myceliophthora thermophila host cell which expresses a recombinant enzymes from Fusarium oxysporum with beta-xylosidase activity. The invention also refers to an enzymatic composition comprising the host cell of the invention and / or the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention. The invention further relates to the use of the host cell of the invention, the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention or the composition of the invention for the degradation of biomass and to a method of producing bioproducts, preferably bioethanol, which comprises the use of the host cell of the invention, the recombinant enzyme with beta-xylosidase activity expressed by the host cell of the invention or the composition of the invention.