43 results about "Beta-Xylosidase" patented technology

The invention discloses a comprehensive utilization method of oil-tea camellia shells. The method comprises the following steps: crushing oil-tea camellia shells, performing alkaline pretreatment, and performing solid-liquid separation to obtain black liquor and solid residues; performing enzymolysis on the solid residues through cellulase to prepare monosaccharide and performing alcohol fermentation, and preparing ethanol; regulating pH of the black liquor, and performing solid-liquid separation to obtain residues and a vanillic aldehyde solution; and performing enzymolysis on the residues through xylanase to prepare an xylooligosaccharide solution and residues, and oxidizing the residues to prepare the vanillic aldehyde. According to the comprehensive utilization method, the solid residues obtained by solid-liquid separation are hydrolyzed by cellulase and further fermented to prepare fuel ethanol by using the condition that the oil-tea camellia shells are pretreated by sodium hydroxide; the black liquor obtained by solid-liquid separation is neutralized to the pH of 6, wherein the supernatant obtained by solid-liquid separation contains high-concentration vanillic aldehyde (lignin oxidization is not required), enzyme hydrolysis is carried out on the precipitation to prepare xylooligosaccharide by employing xylanase containing a small amount of beta-xylosidase, and further the residues obtained by enzymolysis are further oxidized to prepare the vanillic aldehyde. Therefore, according to research of the process, the biomass can be totally utilized for preparing the fuel ethanol, xylooligosaccharide and vanillic aldehyde finally.

The invention discloses a biological compound enzyme preparation and a dark green tea making process using the same for improvement of tea quality, belonging to the field of tea making processes. The compound enzyme preparation contains pectin hydrolase, beta-glucanase, beta-glucosidase, beta-xylosidase and the like; when applied in the dark green tea making process, the compound enzyme preparation can both increase the fragrance of tea and greatly improve the quality of dark green tea, so the compound enzyme preparation has good popularization and application values.

The invention relates to a normal-temperature fungus NJGZ-2 capable of degrading agricultural waste straw, belonging to the agricultural waste resource utilization technology. The class of the strain NJGZ-2 is named penicillium oxalicum, and the culture is collected in the China General Microbiological Culture Collection Center (CGMCC), with collection number CGMCC No.7527. Tests indicate that after the rice straw is inoculated with a spore suspension (1% 10<7> spores/mL), the weight loss ratio of the rice straw is 59.7% after 7 days of 170r/min liquid fermentation culture at 30 DEG C. In the fermentation crude enzyme extraction liquid, the activities of incision enzyme, beta-glucosidase, xylanase, beta-xylosidase and total cellulose are 113.3U/gds, 11.6U/gds, 1,439.7U/gds, 2.0U/gds and 13.8U/gds respectively. The crude enzyme liquid can hydrolyze low-concentration alkali-treated corncob to generate high-concentration reducing sugar (962.2mg of reducing sugar/gram of substrate). According to the invention, the agricultural waste straw is degraded by the microbial fermentation technology and is converted into organic fertilizer, therefore, the waste can be turned into wealth to protect the environment, and application prospects are broad.

The invention discloses a beta-xylosidase mutant and a use thereof. The beta-xylosidase mutant has an amino acid sequence as shown in SEQ ID NO:1. The mutant enzyme has high xylose and glucose tolerance compared with non-mutated beta-xylosidase, and has wide use in degradation of xylo-oligosaccharide.

The invention discloses a beta-xylosidase and an encoding gene and an application thereof. The beta-xylosidase is the following protein in 1 or 2: 1. the protein is formed from an amino acid sequence shown by a sequence 2 in a sequence table; and 2. the protein is formed from an amino acid residue sequence of the sequence 2 in the sequence table through the substitution and/or deletion and/or addition of one or more amino acid residues, has the activity of the beta-xylosidase and is derived from the protein in 1. A recombined colon bacillus formed by leading the encoding gene of the protein into a colon bacillus also belongs to the protection domain of the invention. An experiment proves that the beta-xylosidase produced by the recombined colon bacillus mainly is exoenzyme (the exoenzyme accounts for about 98 percent of the total enzymatic activity), and the enzyme amount of a fermented liquor reaches 103.9U/mL as the maximum when the fermented liquor is fermented for 48 hours. Because most of the combined xylosidase is exoprotein, the combined xylosidase is simply purified, and the recovery rate reaches 61.5 percent. The beta-xylosidase provided by the invention can hydrolyze a corncob steam-exploded fluid, has mild reaction conditions and is environmentally-friendly.

The invention relates to the technical field of biology, in particular to two types of beta-xylosidase from caldicellulosiruptor saccharolyticus and application thereof in biological conversion of natural compounds containing xylosyl modification. Beta-1,4-xylosidase is beta-xylosidase CsXy139A and beta-xylosidase CsXy139B from the same gene cluster of the caldicellulosiruptor saccharolyticus. The beta-xylosidase has the advantages that proved by experiment, the CsXy139A can resist the organic solvent, and the natural compound containing the xylosyltaxol can be hydrolyzed in an organic solvent water solution with higher concentration; meanwhile, the CsXy139A is suitable for the enzyme immobilizing technique; CsXy139B has a good tolerance ability on the hydrolysis products of xylose and glucose, and the heat stability is relatively good; and the two types of beta-xylosidase can perform high-efficiency biological catalyzing on natural drugs containing xylosyl modification.

The invention relates to a chromogenic medium for separating and detecting enterobacter cloacae, and in particular relates to a medium and method for specifically separating and detecting enterobacter cloacae by using beta-xylosidase and a corresponding substrate. The beta-xylosidase chromogenic substrate is decomposed in the presence of a bacterial enzyme to free chromogenic groups out, and colonies present the color of the chromogenic groups, so that the enterobacter cloacae is distinguished from other enterobacteriaceae bacteria. The medium for separating and detecting the enterobacter cloacae, disclosed by the invention, has the advantages of strong specificity, high sensitivity, easiness in operation, simplicity in result judgment and the like, and is applicable in the fields of medical clinical microbial examination, food safety microbial detection, environmental protection and the like.

The invention provides a high-temperature-resistant compound enzyme and applications of the high-temperature-resistant compound enzyme. The high-temperature-resistant compound enzyme is composed of beta-glucosidase and beta-xylosidase. The compound enzyme provided by the invention is strong in specificity, and can completely convert astragaloside, so that the conversion recovery rate of cycloastragenol is improved. The whole process is completed within 3h, the preparation process of cycloastragenol is greatly shortened, and the production efficiency is improved; in the reaction process, the reagents including strong reducing agents, oxidizing agents, strong acid and the like do not need to be added, so that the environmental pollution is reduced; self-making of the two enzymes can be realized, thus the production cost is greatly reduced, and the process is suitable for industrial production.

The invention discloses a method for promoting microbial cells to transport and / or hydrolyze xylo oligosaccharides and application thereof. Experiments prove that cello-oligosaccharide transport protein CDT-2 also has xylo-oligosaccharide transport capacity, beta xylosidase GH43-2 has hydrolysis effect on the xylo-oligosaccharides, according to the method, cdt-2 gene and / or gh43-2 gene are/ is introduced into a microbial strain to obtain a recombinant microbial strain, and compared with a starting strain, the recombinant microbial strain acquires or improves the capability for transporting the xylo-oligosaccharides from extracellular to intracellular and / or hydrolyzing the xylo-oligosaccharides into xylose. By discovery of the xylo-oligosaccharide transport protein CDT-2 and application of the beta xylosidase GH43-2, an idea using modified yeast to directly use the xylo-oligosaccharides derived from hemicellulose degradation for producing biological chemicals is provided, co-fermentation of glucose, xylose, cello-oligosaccharide and xylo-oligosaccharide mixed sugar can be realized by co expression of cdt-2 and gh43-2 and cdt-1 and gh43-1 in brewer's yeast containing xylose metabolic pathway to produce the biological chemicals, and the production cost is greatly saved.

The present invention relates to a host cell, preferably Myceliophthora thermophila cell, which expresses recombinant enzymes with beta-xylosidase activity, an enzymatic composition comprising said cell and/or the recombinant enzyme with beta-xylosidase activity expressed by said cell, the use of this host cell, the recombinant enzyme with beta-xylosidase activity expressed by said cell or the composition for the degradation of biomass and a method of producing bioproducts, preferably bioethanol, which comprises the use of said host cell, the recombinant enzyme with beta-xylosidase activity expressed by said cell or said composition.

The invention discloses a saccharomyces cerevisiae strain expressing beta-xylosidase in cells, which is BSW2AB. The classification is named saccharomyces cerevisiae and filed in 'China General Microbiological Culture Collection Center' with the filed number CGMCC No.5465 on November 17, 2011. The saccharomyces cerevisiae strain is capable of expressing activated beta-xylosidase in cells. The invention provides a method for selecting saccharomyces cerevisiae strainsexpressing activated xylose translocator, and the reaction substrate for selecting the translocator is xylose analogue, namely p-nitrophenyl-beta-D-xyloside. The invention further provides a recombination saccharomyces cerevisiae strain BSW2ABT which has high xylose transfer capability and is obtained by the above method. The classification is named saccharomyces cerevisiae and filed in 'China General Microbiological Culture Collection Center' with the filed number CGMCC No.5464 on November 17, 2011.

Process for treating crop kernels, comprising the steps of: a) soaking kernels in water to produce soaked kernels; b) grinding the soaked kernels; c) treating the soaked kernels in the presence of an effective amount of a beta-xylosidase, wherein step c) is performed before, during or after step b).

The present invention provides a composite enzyme preparation for ramie boiling degumming and a preparation method thereof, wherein the composite enzyme preparation is a mixture of alpha-amylase, low-temperature amylase, alkaline pectinase, endo-beta-1,4-dextranase, neutral protease, oxidase, hemicellulase, beta-xylosidase, papain, keratinase, sorbitol, sodium hydroxide, tetraethyl orthosilicate, sodium metaaluminate and a nanometer desulfurization molecular sieve. According to the present invention, the one-step pickling in the traditional method is eliminated, the one-step alkaline boiling in the traditional process is replaced, a lot of the acid and alkali raw materials are saved, the advantages of mild treatment conditions, low cost, good fiber quality, low environmental pollution and the like are provided, and the efficiency is high compared to the traditional enzyme preparation.

The invention relates to the field of gene engineering, in particular to an encoding gene of glycoside hydrolase for rapid hydrolysizing xylan to generate single xylose, and application of the encoding gene. The nucleotide sequence of the gene is shown in SEQ ID NO. 2. The nucleic acid, which is provided by the invention and is shown in the SEQ ID NO. 2, can be expressed in a large amount in yeast, so that a large amount of the glycoside hydrolase Ttxy43 can be obtained; the glycoside hydrolase Ttxy43 has the activities of beta-xylosidase, endo-beta-xylanase and alpha-L-arabinofuranosidease atthe same time; in addition, the glycoside hydrolase Ttxy43 can directly degrade birch xylan so as to produce a single product xylose; compared with commercial combination of endo-beta xylanase and beta-xylosidase, the glycoside hydrolase Ttxy43 is higher in xylose yield and has a higher commercial application value.

The invention belongs to the technical field of enzymology engineering, and particularly relates to applications of beta-glucosidase SPBGL5 in hydrolysis of xylan polysaccharide substances. Accordingto the present invention, a recombinant Escherichia coli strain containing a beta-glucosidase gene spbgl5 from Sphingomonas sp. ATCC 31461 is constructed, wherein the gene is subjected to induced expression and nickel affinity chromatography purification in Escherichia coli to obtain an expression product beta-glucosidase SPBGL5; and the obtained beta-glucosidase SPBGL5 has the activities of beta-glucosidase, further has the activities of beta-xylosidase and beta-arabinosidase, can separately hydrolyze beech xylan, birch xylan, oat xylan, corncob xylan, wheat araboxylan and lichenin, and has great application potential in degradation of various xylan polysaccharide substances.

A novel glycosyl hydrolase with activities of beta-xylosidase and beta-glucosidase is provided. Said glycosyl hydrolase can convert 7-xylosyltaxane compounds to 7-hydroxyltaxane compounds.

A thermostable β-xylosidase including a β-xylosidase catalytic domain, the β-xylosidase catalytic domain including:

• • (A) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1;
  • (B) a polypeptide including an amino acid sequence in which at least one amino acid is deleted, substituted, or added in the amino acid sequence represented by SEQ ID NO: 1, and having hydrolytic activity using p-nitrophenyl-β-D-xylopyranoside as a substrate at least under conditions of a temperature of 85° C. and a pH of 6.0; or
  • (C) a polypeptide including an amino acid sequence having at least 80% sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having hydrolytic activity using p-nitrophenyl-β-D-xylopyranoside as a substrate at least under conditions of a temperature of 85° C. and a pH of 6.0.

The present invention relates to a Myceliophthora thermophila cell, which expresses a nucleotide sequence that codifies a recombinant beta-xylosidase enzyme comprising an amino-acid sequence having at least 70% identity with SEQ ID NO: 1, an enzymatic composition comprising said cell and/or the recombinant enzyme with beta-xylosidase activity expressed by said cell, the use of this host cell, the recombinant enzyme with beta-xylosidase activity expressed by said cell or the composition for the degradation of biomass, and a method of producing biological products, preferably bioethanol, comprising the use of said host cell, the recombinant enzyme with the beta-xylosidase activity expressed by said cell or said composition.

Provided are certain glycosyl hydrolase family 3 (GH3) beta-xylosidases engineered to acquire beta-glucosidase activities. Provided also are compositions comprising such multi-functional GH3 enzymes and methods of use or industrial applications thereof.

A method for determining the vase life or storage history of one or more cut flowers, wherein the method comprises assaying a test sample obtained from the one or more cut flowers for one or more of: (a) an indicator representative of xylose concentration; (b) an indicator representative of β-xylosidase expression; and (c) an indicator representative of β-xylosidase activity; to determine a value for (each of) the one or more indicators in the test sample.