Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Beta-xylosidase mutant and use thereof

A mutant, glucosidase technology, applied in the field of enzymology, can solve the problems of strong randomness, large workload, poor enzymology properties, etc., and achieve the effect of wide application prospects

Active Publication Date: 2014-02-26
GUANGXI UNIV
View PDF3 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The breeding of microbial strains is one of the most commonly used and simplest methods in industrial production and academic research, but it is often difficult to screen ideal strains due to heavy workload and strong randomness
Protein engineering is an emerging method that utilizes molecular biology and bioinformatics methods to conduct directed mutation and rational transformation of proteins to obtain ideal proteins or enzymes. Its advantages lie in relatively small workload and high probability of success, but Enzymatic properties of most mutant proteins were unchanged or worse

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Beta-xylosidase mutant and use thereof
  • Beta-xylosidase mutant and use thereof
  • Beta-xylosidase mutant and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of embodiment 1β-xylosidase mutant

[0027] Materials: Escherichia coli strain XL10-gold and Escherichia coli M15 were purchased from Stratagene; Ni-NTA protein histidine purification medium was purchased from Intrivogen; restriction enzymes and modification enzymes were purchased from TaKaRa and MBI ; Xylooligosaccharides and xylose were purchased from Sigma; the rest of the reagents were conventional reagents purchased from the market unless otherwise specified.

[0028] 1) Cloning of β-xylosidase gene Exyl

[0029] A gene sequence encoding a putative glycosyl hydrolase (YP_003612882.1) of Enterobacter cloacae subsp.cloacae ATCC13047 was found from the GenBank gene database. Design the upstream primer Exyl-1: 5'-CTGAGATCTACTGCAATCTATAAGGACGCGGGAC-3' (containing a BglII restriction site) and the downstream primer Exyl-25'-ACTAAGCTTCTACGCGTGCTGAACCTGACAGGTG-3' (containing a HindIII restriction site), through the polymerase chain The β-xylosidase gene E...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses a beta-xylosidase mutant and a use thereof. The beta-xylosidase mutant has an amino acid sequence as shown in SEQ ID NO:1. The mutant enzyme has high xylose and glucose tolerance compared with non-mutated beta-xylosidase, and has wide use in degradation of xylo-oligosaccharide.

Description

technical field [0001] The invention belongs to the field of enzymology, relates to a beta-xylosidase mutant, and also relates to the use of the mutant in degrading xylooligosaccharides. Background technique [0002] Xylan is the main component of hemicellulose, accounting for 30-35% of the total dry weight of hemicellulose. It is widely distributed in the cell walls of higher plants. It is a renewable resource with a huge stock in nature, and it is also easier to extract, A class of hemicelluloses that are degraded and utilized. Xylanase is a kind of complex enzyme system that can hydrolyze xylan to generate xylose, mainly composed of endoxylanase (EC.3.2.1.8) and β-xylosidase (EC3.2.1.37) (Beg Q.K., Kapoor M., Mahajan L., et al. 2001, Microbial xylanase and their industrial applications: A review. Appl. Microbiol Biotechnol., 56:326-338.). [0003] β-Xylosidase (β-D-xylosidase) is an exohydrolase, which cooperates with endoxylanase to degrade the degradation product of e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N15/56C12N1/15C12N1/19C12N1/21C12P19/14
CPCC12N9/2434C12Y302/01037
Inventor 杜丽琴黄金群王子龙王金佩黄日波张志凯韦宇拓
Owner GUANGXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com