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Chromogenic medium for separating and detecting enterobacter cloacae

A culture medium and tryptone technology, applied in the direction of microbe-based methods, bacteria, microbe determination/testing, etc., can solve the problems of false positives, inability to distinguish Enterobacter cloacae, missed detection, etc., and achieve strong specificity and result judgment Simple, reliable separation and detection of effects

Active Publication Date: 2014-05-28
BEIJING JUNLIKANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, none of the above media can distinguish E. cloacae from other Enterobacteriaceae
[0005] To sum up, the current technical bottleneck of the culture medium for the isolation and detection of Enterobacter cloacae lies in: on the one hand, it is difficult to accurately isolate Enterobacter Difficult to distinguish from other Enterobacter species, causing false positive results

Method used

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  • Chromogenic medium for separating and detecting enterobacter cloacae
  • Chromogenic medium for separating and detecting enterobacter cloacae
  • Chromogenic medium for separating and detecting enterobacter cloacae

Examples

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Effect test

Embodiment 1

[0056] Solid plate preparation: chromogenic medium 1 to 7 described in the following examples 2-8 were weighed according to the names and contents of the components described therein, and each component of the above-mentioned medium was added to deionized water, and stirred , heat and boil until completely dissolved, adjust the pH to 6.8±0.2, wait to cool to 45-55°C, pour into a flat plate, wait to cool to room temperature, and use it after it is completely solidified.

[0057] Preparation of experimental strains and application of medium: inoculate the experimental strains in the following Table 1 in TSB broth, culture at 35±1°C for 24 hours, and then inoculate them into the above-prepared medium with inoculation needles, respectively. Incubate at 35±1°C for 24-48h.

Embodiment 2

[0059] A chromogenic medium for the isolation and detection of Enterobacter cloacae. Each 1000mL medium contains tryptone 8g, plant peptone 8g, glucose 3g, lactose 3g, sucrose 3g, sodium chloride 5g, agar 13g, 5-bromo-4chloro-3-indole-β-xyloside 0.1g, 5 -Bromo-6-chloro-3-indole-β-galactoside 0.1g, bile salt 2g, ampicillin 5mg, the balance is water, pH6.8±0.2.

[0060] Prepare and use according to the method described in Example 1, all Enterobacter cloacae experimental strains grow into blue-green bacterial strains, Cronobacter and Gram-positive bacteria are inhibited, Klebsiella, Citrobacter, Escherichia coli, Enterobacter aerogenes showed pink colonies, and other colonies were white. Enterobacter cloacae was more obvious on chromogenic medium 1 than chromogenic medium 2-7.

Embodiment 3

[0062] Chromogenic medium II for the isolation and detection of Enterobacter cloacae. Each 1000mL medium contains tryptone 8g, plant peptone 9g, glucose 2g, L-arabinose 5g, raffinose 4g, sodium chloride 5g, agar 15g, 5-bromo-4chloro-3-indole-β-xyloside 0.3g, bile salt 3g, ampicillin 10mg, the balance is water, pH6.8±0.2.

[0063] Prepare and use according to the method described in Example 1, all experimental strains of Enterobacter cloacae grow into blue-green strains, Cronobacter and Gram-positive bacteria are inhibited, other colonies are white, and Enterobacter cloacae is grown in the chromogenic medium Second, it is easy to identify.

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Abstract

The invention relates to a chromogenic medium for separating and detecting enterobacter cloacae, and in particular relates to a medium and method for specifically separating and detecting enterobacter cloacae by using beta-xylosidase and a corresponding substrate. The beta-xylosidase chromogenic substrate is decomposed in the presence of a bacterial enzyme to free chromogenic groups out, and colonies present the color of the chromogenic groups, so that the enterobacter cloacae is distinguished from other enterobacteriaceae bacteria. The medium for separating and detecting the enterobacter cloacae, disclosed by the invention, has the advantages of strong specificity, high sensitivity, easiness in operation, simplicity in result judgment and the like, and is applicable in the fields of medical clinical microbial examination, food safety microbial detection, environmental protection and the like.

Description

technical field [0001] The invention relates to a chromogenic medium for isolating and detecting Enterobacter cloacae, in particular to a chromogenic medium for isolating and detecting Enterobacter cloacae. Background technique [0002] Enterobacter cloacae (Enterobacter cloacae) belongs to the genus Enterobacteriaceae, widely exists in nature, and is one of the common strains that can be detected in human and animal feces, water, soil, and plants. As an opportunistic pathogen, Enterobacter cloacae can cause a variety of bacterial infectious diseases, such as skin and soft tissue infections, urinary tract infections, respiratory tract infections, and sepsis. With the widespread use of cephalosporins, since Enterobacter cloacae can produce extended-spectrum β-lactamases (ESBLs) and Amp C enzymes, Enterobacter cloacae, which is highly prone to drug resistance, is harmful to humans. The danger is growing. In addition, "Science and Technology Daily" (2012-12-21 edition) also r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/10C12R1/01
Inventor 赵贵明陈颖赵勇胜杨海荣刘洋王娉胡玥
Owner BEIJING JUNLIKANG BIOTECHNOLOGY CO LTD
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