The invention discloses loop-mediated isothermal amplification (LAMP) primers, a kit and a detection method for detecting common carbapenemase genes of
gram negative
bacilli. In the LAMP primers disclosed by the invention,
klebsiella pneumoniae carbapenemase (KPC) and new delhi metallo-b-lactamase (NDM) primer groups can detect all subtypes except for NDM-10;
hypoxanthine nucleotide (IMP) and
vimentin (VIM) primer groups can detect common subtypes at home and abroad. The LAMP kit built by the invention is applied to joint detection of KPC, NDM, IMP and VIM genes, can cover the common carbapenemase genes of non-fermentative
bacteria and
enterobacteriaceae, can accurately and quickly screen the common carbapenemase genes, and has great
clinical significance for timely detecting and further controlling fulminant epidemic caused by propagation of the carbapenemase genes in
enterobacteriaceae. The kit disclosed by the invention is high in detection sensitivity and the minimum detection limits of the KPC, NDM, IMP and VIM genes can reach 100 CFU / reaction.