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Methods for improving efficiency of simultaneous saccharification and fermentation reactions

A technology for synchronous saccharification and fermentation and fermentation products, which is applied in the direction of using microorganisms, biochemical equipment and methods, fermentation, etc., and can solve problems such as lengthy

Inactive Publication Date: 2017-10-24
DANISCO US INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, typical SSF responses are very lengthy and prolonged, eg several days, to reach moderate ethanol concentrations (see, eg, Kadam et al., 2004, Biotechnol. Progr. 20(3):705)

Method used

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  • Methods for improving efficiency of simultaneous saccharification and fermentation reactions
  • Methods for improving efficiency of simultaneous saccharification and fermentation reactions
  • Methods for improving efficiency of simultaneous saccharification and fermentation reactions

Examples

Experimental program
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Effect test

example 1

[0333] 7. Example 1: Analysis of Exp formation in SSF reactions

[0334] 7.1. Materials and methods

[0335] 7.1.1. Substrates

[0336] The substrates used in this work are listed below. The cellulose, xylan and lignin components of the pretreated substrates are also listed. Composition analysis utilized the standards detailed in "NREL protocols for Standard Biomass Analytical Procedures" (available at http: / / www.nrel.gov / biomass / pdfs / 42618.pdf) Assay performed:

[0337] Corn cob pretreated with dilute ammonia water . Corn cobs are pretreated prior to enzymatic hydrolysis according to, for example, U.S. Patent Application Publication Nos. The methods and processing scope described in patent application publication WO06 / 110901A2 were carried out (unless otherwise indicated).

[0338] The composition of the substrate includes : 34.8% cellulose, 29.2% xylan, 12.8% lignin.

[0339] Bagasse pretreated with dilute sulfuric acid . This substrate is produced and su...

example 2

[0401] 8. Example 2: Hemicellulase leads to reduction of exp

[0402] The following example demonstrates that the addition of certain hemicellulases reduces EXP in SSF reactions.

[0403] 8.1. Fv43D leads to reduced EXP in Zymomonas SSF

[0404] EXP formation typically consumes xylose (either directly, or from xylobiose or other xylo-oligomers) and ethanol in an equimolar ratio. This consumption mechanism directly leads to significantly reduced ethanol production because many microorganisms, including Zymomonas, are unable to degrade, ferment, or otherwise utilize EXP. One (1) gram of EXP was calculated to be equivalent to a loss of 0.688 g of ethanol produced (assuming xylose was fermented to ethanol at a rate of 0.51 g / g xylose). Thus, preventing the formation of EXP can lead to a consequent increase in ethanol production. have been found, such as Figure 10 It was shown that addition of the Fusarium verticillium beta-xylosidase Fv43D at an amount of only 1 mg / g xyla...

example 3

[0418] 9. Example 3: EXP formation mechanism

[0419] Under SSF conditions ( Figure 10 and 11 ), the EXP production in the presence of Verticillium β-xylosidase Fv3A was approximately 4-fold higher than in the presence of Verticillium β-xylosidase Fv43D, such observations inspired Efforts have been made to understand the mechanism of EXP formation.

[0420] There are potentially two possible pathways for EXP formation: (1) by transglycosylation; or (2) by reverse hydrolysis. Each of these pathways is described below:

[0421] 1) Transglycosylation: X-O-X+EtOH→X-O-Et+X

[0422] 2) reverse hydrolysis:

[0423] According to Drouet et al. (1994, Biotech. & Bioeng. 43: 1075-1080), the transglycosylation mechanism is the more rapid of the two. Only the β-anomer is formed in the transglycosylation reaction (see Figure 29 ) implies that the reaction is catalyzed by an enzyme that acts on the substrate in a way that maintains the anomeric configuration, where the sugars ar...

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Abstract

The present disclosure is directed, in a first aspect, to the use of inverting beta-xylosidase enzymes to reduce byproduct formation and increase the yield of fermentation products, as well as, in a second aspect, to the use of retaining beta-xylosidase enzymes to improve production of alkyl-beta-xylopyranoside compounds, in a simultaneous saccharification and fermentation reactions.

Description

[0001] This application is a divisional application of Chinese patent application 201080058488.3 entitled "Method for Improving Synchronous Saccharification and Fermentation Reaction Efficiency" submitted by the applicant on December 17, 2010. [0002] 1. Cross references to related patent applications [0003] This patent application claims the benefit of US Provisional Application 61 / 289,917, filed December 23, 2009, which is incorporated by reference in its entirety. 2. Technical field [0004] The present invention generally relates to methods and compositions for increasing the yield of products from simultaneous saccharification and fermentation reactions. 3. Background technology [0005] Since the oil crisis of the 1970s due to OPEC's reduction in oil output, researchers have focused intensely on the bioconversion of renewable lignocellulosic biomass to fermentable sugars that are subsequently fermented to produce Alcohols (such as ethanol or "bio-ethanol") that ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P7/04C12P7/16C12P7/18C12P19/14C12N1/22
CPCC12N1/22C12P7/04C12P7/10C12P7/16C12P7/18C12P19/14C12N9/2434C12N9/2477C12N9/248C12P19/44C12Y302/01037Y02E50/10C12N15/80C12P7/06C12P19/02
Inventor C·古提尔雷兹C·米奇森T·T·黄B·A·戴纳P·J·费根W·D·希茨
Owner DANISCO US INC
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