Method of preparing heat-proof xylanase, heat-proof beta-xylosidase or heat-proof beta-glucosidase

A technology of glucosidase and xylanase, applied in the direction of hydrolase, bacteria, etc., to achieve the effect of reducing initial hardness, specific hydrolysis substrate, and simple and easy method
CN100575483CInactive Publication Date: 2009-12-30CHINA AGRI UNIV

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2009-12-30
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a making method of heat-proof xylanase, heat-proof beta-xylosidase or heat-proof beta-glucosidase, which comprises the following steps: adopting agricultural waste as carbon source; fermenting Paecilomyces thermophila J18 at 40-60 deg.c to obtain the ferment liquid with heat-proof xylanase, heat-proof beta-xylosidase or heat-proof beta-glucosidase; purifying; obtaining electrophoretic grade product.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to a method for preparing heat-resistant xylanase, heat-resistant β-xylosidase or heat-resistant β-glucosidase. Background technique

[0002] Xylanase (Xylanase, EC 3.2.1.8) is an important class of hydrolase, which mainly acts on the β-1,4-glucosidic bond on the main chain of xylan in an endo-cutting manner, and the hydrolyzed products are mainly Oligosaccharides and xylose. At present, there are relatively many studies on xylanase of thermophilic fungi abroad. Haltrich et.al. (Enzyme Microb. Technol. 15: 854-860) used Schizophylum commune BT2115 to ferment microcrystalline cellulose as a carbon source. The carbohydrase activity reached 4839U / mL fermentation broth, which is the highest level reported so far for xylanase produced by liquid fermentation. There have been some research reports on fungal xylanase production in China, but there are few research reports on fungal xylanase production and thermophilic fungal strains. L...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More