Production method for high-efficiency phosphors-resolving penicillium oxalicum agent with heavy metal tolerance characteristic
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A technology of Penicillium oxalicum, production method, applied in the field of environmental biology
Inactive Publication Date: 2014-04-02
BEIJING FORESTRY UNIVERSITY
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At present, there is no literature report on the production method of high-efficiency phosp...
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Embodiment 1
[0025] Embodiment 1: the production of highly efficient penicillium oxalic acid phospholyticum resistant to heavy metals
[0026] 1. Medium preparation:
[0027] 1) Strain preservation medium (solid, 1 L): 5 g of sucrose, 18-20 g of agar, 40 g of leachate obtained by boiling and filtering potatoes, adding sweet potato starch wastewater to make up to 1 L, natural pH;
[0028] 2) Strain activation medium (liquid, 1 L): 5 g of sucrose, leaching solution obtained by boiling and filtering 40 g of potatoes, adding sweet potato starch wastewater to make up to 1 L, natural pH;
[0029] 3) Seed medium (liquid, 10 L): peptone 10 g, glucose 20 g, KH 2 PO 4 10 g, MgSO 4 ·7H 2 0.5 g, add sweet potato starch waste water and settle to 10 L, natural pH;
[0030] 4) Fermentation medium (liquid, 200 L): KH 2 PO 4 200 g, KH 2 PO 4 100 g, MgSO 4 ·7H 2 O 20 g, add sweet potato starch waste water to 200 L, natural pH;
[0031] All the above media were sterilized at 121°C for 15-30 mi...
Embodiment 2
[0037] Embodiment 2: The growth-promoting effect of high-efficiency phospholytic penicillium oxalic acid fungus on Viburnum
[0038] After mixing the prepared oxalic acid bacterium agent with the soil at a ratio of 1:20, 1:15, 1:10, and 1:5, plant the germinated Viburnum seeds, and add 1:10 without The chaff residue fermented by Penicillium oxalicum was used as the control. 5 pots were treated in each pot, and 3 seedlings were planted in each pot, which were placed in the greenhouse for unified management and watered regularly. After 60 days of planting and emergence of Viburnum, the growth conditions are shown in Table 1. It can be seen that the plants inoculated with the Penicillium oxalicum inoculant, the dry weight of roots and seedlings of each addition ratio and the height of seedlings and root length all increase compared with the matched group. root length and seedling height showed an increasing trend, but when the addition ratio exceeded 1:10, the dry weight of roo...
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Abstract
The invention relates to a production method for producing a high-efficiency phosphors-resolving penicillium oxalicum agent with heavy metal tolerance characteristic by utilizing sweet potato starch waste water and waste mushroom residue slag, which belongs to the field of environmental biotechnology. The high-efficiency penicillium oxalicum is separated and screened from topsoil of a lead zinc ore at Huayuan county of the Xiangxi autonomous region in Hunan province in the laboratory, and has the characteristic of tolerating multiple metals. According to the method, the phosphors-resolving penicillium oxalicum agent with heavy metal tolerance characteristic is produced mainly by utilizing sweet potato starch waste water and waste mushroom residue slag as main culture medium materials through adopting a liquid-solid combined fermentation method. The production method for the penicillium oxalicum agent comprises six steps of culture medium preparation, strain activation, preparation of liquid seeds, liquid-state fermentation, solid-state fermentation and aftertreatment. According to the technical scheme, the production method provides a new approach for resource utilization of the high-concentration sweet potato starch waste water and waste mushroom residue slag, and the produced penicillium oxalicum agent has the characteristics of being environment-friendly and low in production cost, and the product also has the functions of obviously improving the quality of soil environment, and promoting the growth of plants.
Description
technical field [0001] The invention belongs to the field of environmental biology technology, and in particular relates to a method for producing a high-efficiency phospholytic oxalic acid oxalic acid bacterium resistant to heavy metals by using sweet potato starch wastewater and fungus chaff waste residue as main culture medium raw materials through liquid-solid joint fermentation. Background technique [0002] Phosphorus is an essential element for plant growth, second only to nitrogen in importance. However, the nitrogen elements needed by plants can be realized by converting nitrogen in the air into nitrate nitrogen through nitrogen-fixing bacteria and nitrifying bacteria in the soil and roots, but the required phosphorus elements can only be met by absorbing soluble phosphorus in the soil. In the soil, especially in the mining area, due to the presence of a large amount of heavy metals, and the heavy metals are extremely easy to form insoluble phosphoric acid compounds...
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