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Penicillium oxallcum Po-5 strain and application thereof

A technology of Penicillium oxalate and po-5, applied in fungi, enzymes, biochemical equipment and methods, etc., can solve problems such as lack of high-yielding strains, and achieve the effects of reducing environmental pressure, reducing production costs, and optimizing processes

Inactive Publication Date: 2011-08-17
ZHEJIANG NORMAL UNIVERSITY
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Problems solved by technology

However, there is still a lack of high-yield strains that can be used for pectinase production and have independent property rights

Method used

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  • Penicillium oxallcum Po-5 strain and application thereof
  • Penicillium oxallcum Po-5 strain and application thereof
  • Penicillium oxallcum Po-5 strain and application thereof

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Embodiment Construction

[0031] 1. Po-5 strain.

[0032] The strain used for liquid fermentation to produce pectinase was Penicillium oxalicum ( Penicillium oxalicum ) Po-5 strain, which was screened from the sludge in Jinhua Dongyang lotus pond, and its ITS gene sequence has been registered in Genbank with the accession number HQ680452. A phylogenetic tree was established based on the ITS sequence of the Po-5 strain ( figure 1 ).

[0033] Penicillium oxalicum used in the present invention ( Penicillium oxalicum ) Po-5 strain has been preserved in the China Center for Type Culture Collection, the preservation number is: CCTCC M 2010350, and the preservation date is December 14, 2010.

[0034] Culture characteristics of Po-5 strain: Penicillium oxalicum Po-5 strain formed a typical colony on PDA medium at 28°C for 3 days. The colony was blue-green, with white edges, 19 mm in diameter, regular outline, and fluffy appearance shape. Observed under a microscope, the hyphal cells are filamentously int...

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Abstract

The invention belongs to the field of microbial fermentation and particularly relates to a penicillium oxallcum Po-5 strain capable of generating pectinase and a method for producing the pectinase by liquid fermentation. The penicillium oxallcum Po-5 strain is preserved in the China center for type culture collection on Dec14th in 2010 and the preservation number is CCTCC (China Center for Type Culture Collection) NO.M2010350. The strain with high yield of pectinase is selected from the natural environment and the pectinase is produced in a liquid fermentation mode so as to reduce the production cost of the pectinase, optimize the process and reduce the environment stress. The strain uses natural orange skins, rice brans and the like with low prices as a culture medium matrix, the activity of the pectinase obtained by fermentation can achieve 1.39*105U<min-1>< ml-1> and the penicillium oxallcum Po-5 strain belongs to a high-yield pectinase strain. The pectinase prepared by carrying out liquid fermentation on the Po-5 strain can keep good stability at the temperature of 30 to 50 DEG C when the pH (potential of hydrogen) value is equal to about 5.0.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, in particular to a strain of Penicillium oxalicum producing pectinase ( Penicillium oxalicum ) Po-5 strain and its liquid fermentation method for producing pectinase. Background technique [0002] The research and application of pectinase has a history of more than 70 years. Now many countries have commercial pectinase preparations for sale, and the varieties are becoming more and more diversified. Its output has ranked among the top four enzyme preparations in the world. [0003] Pectinase has a wide range of uses and can be well applied in many fields. In the fruit processing industry, it can destroy the stability of the suspended matter in the juice, make it coagulate and precipitate, clarify the juice, and reduce the viscosity of the juice. In the feed production industry, pectinase can reduce the viscosity of feed and promote the digestion and absorption of feed in the digestive trac...

Claims

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Application Information

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IPC IPC(8): C12N9/26C12R1/80C12N1/14
Inventor 蒋冬花蓝丽精宋迤明韦风
Owner ZHEJIANG NORMAL UNIVERSITY
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