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Preparation of composite microbial agent for coal gangue artificial ecological matrix and application thereof

A compound microbial agent and artificial ecology technology, applied in the field of environmental biology, can solve the problems of small number and types of active microorganisms, difficulty in using nutrients directly by plants, increasing the number of active microorganisms in the artificial matrix of coal gangue, etc., to achieve the improvement of the types of active microorganisms and quantity, improving soil environmental quality, and improving physical and chemical properties

Active Publication Date: 2020-04-10
北京优生基生态科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is: to provide a compound microbial functional bacterial agent for coal gangue artificial ecological matrix, to solve the problems that the nutrients in coal gangue are difficult to be directly utilized by plants, and the types and quantities of active microorganisms are small, and can be used in coal gangue. Prepare the artificial ecological matrix to make it mature, in which the refractory nutrients are effectively released, increase the number of active microorganisms in the artificial matrix of coal gangue, and realize the resource utilization effect of coal gangue

Method used

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  • Preparation of composite microbial agent for coal gangue artificial ecological matrix and application thereof
  • Preparation of composite microbial agent for coal gangue artificial ecological matrix and application thereof
  • Preparation of composite microbial agent for coal gangue artificial ecological matrix and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Study on antagonism between strains

[0021] The above 5 strains of actinomycetes were streaked with Pseudomonas on a solid medium containing an equal volume of Gao's No. I and LB. The 5 strains of actinomycetes were streaked with Penicillium oxalicum on a solid medium containing equal volumes. On the Gao's No. I and PDA solid medium, streak Pseudomonas and Penicillium oxalicum on solid medium containing equal volume of LB and PDA, streak in pairs but not intersect, culture for 4 days at 30℃ . If an aseptic zone is formed at the intersection of two bacteria, it means that a zone of inhibition is formed between the two bacteria. The two bacteria are antagonistic to each other and cannot be combined together. If the two bacteria do not form a sterile zone, it means that the two bacteria are not antagonistic to each other. combination. From the results, there is no antagonistic effect between the various strains, and they will not interfere with each other and aff...

Embodiment 2

[0026] Example 2 The effect of different strain combinations on the physical and chemical indexes and microorganisms of coal gangue artificial ecological matrix

[0027] According to the functional microbial agent of the present invention, 5 species of actinomycetes (including Streptomyces thermophilus, Streptomyces thermoamylase, Streptomyces thermophilic carbon monoxide, Streptomyces leucocephalus, Streptomyces common thermophilus), 1 species of bacteria (false Monospora), a mold (penicillium oxalicum). Five strains of Streptomyces were inoculated into sterile ISP2 liquid medium, pH 7.2-7.4, and cultured at 50°C and 120rpm in a shaking table. One strain of Pseudomonas was inoculated into sterile LB liquid medium, pH 7.2- 7.4. Cultivate for 12 hours at 30°C and 150 rpm on a shaker. Inoculate a strain of Penicillium oxalicum in a potato liquid medium with natural pH and culture for 96 hours at 28°C and 170 rpm on a shaker. Set up different strain combinations: treat 1,5 species ...

Embodiment 3

[0030] Example 3 Preparation of composite bacterial agent

[0031] (1) Strain activation: Take the slant preserved at 4°C of the microorganism of the present invention, and inoculate 5 kinds of actinomycetes to the Gao's No. I solid plate medium respectively, culture for 3 days in a constant box at 50°C, and inoculate one kind of bacteria to the LB solid plate Culture medium, cultured in a constant box at 35°C for 2 days, inoculated one mold to the PDA solid medium, and cultured in a constant box at 28°C for 4 days to achieve strain activation.

[0032] (2) Seed solution preparation: Transfer the 5 kinds of actinomycetes to the 200mL sterile ISP2 liquid medium, pH 7.2-7.4, 50°C, 120rpm shaker. Culture under conditions, transfer 1 species of bacteria to 200mL sterile LB liquid medium, pH 7.2-7.4, 35℃, 150rpm shaker conditions, transfer 1 species of mold to 400mL sterile PD liquid medium, natural pH, 28℃, 180rpm shaking table conditions, high temperature Streptomyces purpurea, Strep...

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Abstract

The invention discloses a composite microbial agent for a coal gangue artificial ecological matrix, which comprises five actinomycetes, streptomyces thermoviolaceus, streptomyces thermodiastaticus, streptomyces thermocarboxydus, streptomyces albidoflavus and streptomyces thermovulgaris, and one bacterium is pseudomonas sp. and one mould is penicillium oxalicum. The preparation process of the composite microbial agent comprises the following four steps: activating strains, preparing a seed solution, preparing a liquid composite microbial inoculum and preparing a solid microbial inoculum. According to the technology, the problems that microorganisms in the coal gangue are lacked, and nutrients are not beneficial to plant absorption and utilization are solved, a new way is provided for resource utilization of the coal gangue and landscaping waste, and the preparation has wide application prospects in the aspects of treatment, resource utilization and the like of the coal gangue.

Description

Technical field [0001] The invention belongs to the field of environmental biotechnology, and specifically relates to the preparation and application of a composite microbial inoculant used for coal gangue artificial ecological substrate. Background technique [0002] Coal gangue is a co-product in coal production, and its output is 10%-20% of coal production. It is one of the largest industrial solid wastes in my country. The natural piling of coal gangue not only takes up a lot of land, but also causes irreversible pollution to the surrounding environment and groundwater. The main treatment methods of coal gangue include landfill, coal gangue power generation, industrial extraction of certain substances, and brick and cement production in the construction industry. In recent years, the use of coal gangue as a component of soil amendment or the preparation of compound fertilizers has also begun to be reported, but the general amount is relatively small, and a large amount of co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05G3/80A01G24/20A01G24/10C12R1/465C12R1/38C12R1/80
CPCC12N1/20C12N1/14C05G3/00A01G24/20A01G24/10Y02W30/40
Inventor 彭霞薇代兴和乔浩亮周金星
Owner 北京优生基生态科技有限公司
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