Streptomyces globisporus microbial agent for controlling Alternaria alternate and preparation method thereof

Abstract

The invention discloses a biological agent for controlling a tobacco brown spot. The production strain of the biological agent is sorted and named as Streptomyces globisporus AM6 and the preserving number thereof is CGMCCNo.1701. The prescriptions of the solid and the liquid mediums of the strain are as follows: (1) the incline medium of a test tube is as follows: 200g of fresh potatoes, 20g of dextrose, 17 to 20g of agar, 1000ml of distilled water as well as a natural pH; (2) the prescription of a liquid fermentation medium is as follows: 10.0g of bean powder, 20.0g of cane sugar, 2g of dipotassium hydrogen phosphate, 0.02g of green copperas, 0.5g of calcium carbonate, 0.05g of bitter salt, 1000ml of tap water as well as a natural pH. The invention is prepared into the biological agent by the test tube culturing of the strain and the fermentation culturing of a table concentrator. Test results show that the biological agent provided by the invention has excellent controlling effects on the tobacco brown spot.

Description

technical field [0001] The present invention relates to biopesticide, more specifically, the present invention relates to a kind of bacterial agent for preventing and treating tobacco red spot disease and its preparation method. Background technique [0002] Tobacco is an important foliar economic crop in my country, and it is one of the crops with the most serious disease damage. From sowing to harvesting, the whole process can be infected by pathogens. Tobacco Brown Spot Disease (Tobacco Brown Spot Disease) is an important leaf disease in the late mature stage of tobacco leaves. It commonly occurs in various smoking areas and is the main disease of tobacco in my country. In recent years, red spot disease has also developed in Hunan, Anhui, Guizhou, Yunnan, Shaanxi, Liaoning, Zhejiang, Guangdong, Fujian, Heilongjiang, Jilin and other provinces in China, becoming the most harmful leaf spot disease. 20% to 30%, the severe incidence rate reaches 90%, and the output value is r...

AI Technical Summary

Problems solved by technology

[0003] Tobacco red spot disease is a typical airborne disease. At present, agricultural measures such as health care cultivation and large doses of chemical pesticides are mainly used for prevention and control in China. There is a lack of resistant varieties for regional cultivation; Prevention and control, the cost of medication is increasing year by year, and the effect of prevention and control is gradually weakening. Some pathogenic fungi have developed drug resistance, and the difficulty of prevention and control has also increased.

[0004] Because chemical agents are very easy to cause phytotoxicity, they are also easily washed away by rainwater when applying pesticides, and there is a lack o...

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