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Method for sterilizing shrimp eggs without affecting hatchability

A disinfection method and hatching rate technology, applied in the directions of disinfectants, botanical equipment and methods, climate change adaptation, etc., can solve problems such as reducing the activity of shrimp eggs, and achieve the effects of low survival rate, convenient operation and poor quality.

Active Publication Date: 2017-11-14
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The prior art, such as the Chinese invention patent with the authorized announcement number CN 103960182 B, discloses a method of using shrimp eggs to culture in vitro to establish a family of white prawns. Soaking and disinfection in potassium sea water solution reduces the impact of pathogens, but potassium permanganate also greatly reduces the activity of shrimp eggs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] A method for disinfecting shrimp eggs that does not affect hatchability, comprising:

[0014] 1) De-egging: Select healthy broodstock shrimp to ensure that the shrimp eggs are in the period after the eye point pigmentation period, and use sterilized tweezers to separate the shrimp eggs from the broodstock abdomen;

[0015] 2) Preparation of disinfectant: Take 3 parts of Streptomyces coccidioides fermentation broth, add 3 parts of citric acid, then add 4 parts of crab shell extract, stir for 20 minutes, then add 5 parts of limonene, 1 part of β-phellandrene, 2 parts of Caryophyllene, stirred at 35°C for 1.5h to obtain a disinfectant;

[0016] 3) Preparation of disinfectant: Take 3 parts of disinfectant, add 45 parts of water, stir for 15 minutes to mix;

[0017] 4) Disinfection: Take 5 parts of shrimp eggs, add the disinfectant solution prepared in step 2), soak for 6 hours, and take out.

[0018] The composition and parts by weight of the disinfectant are: 3 parts of ...

Embodiment 2

[0022] A method for disinfecting shrimp eggs that does not affect hatchability, comprising:

[0023] 1) De-egging: Select healthy broodstock shrimp to ensure that the shrimp eggs are in the period after the eye point pigmentation period, and use sterilized tweezers to separate the shrimp eggs from the broodstock abdomen;

[0024] 2) Preparation of disinfectant: Take 8 parts of Streptomyces coccidioides fermentation broth, add 6 parts of citric acid, then add 7 parts of crab shell extract, stir for 25min, then add 10 parts of limonene, 3 parts of β-phellandrene, 5 parts of Caryophyllene, stirred at 40°C for 2 hours to obtain a disinfectant;

[0025] 3) Preparation of disinfectant: Take 1 part of disinfectant, add 40 parts of water, stir for 15 minutes to mix;

[0026] 4) Disinfection: Take 2 parts of shrimp eggs, add the disinfectant solution prepared in step 2), soak for 3 hours, and take out.

[0027] The components of the disinfectant and their parts by weight are: 8 parts...

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Abstract

The invention discloses a method for disinfecting shrimp eggs without affecting hatchability, which comprises the steps of loosening eggs, preparing a disinfectant, preparing disinfectant liquid and disinfecting, wherein the disinfectant is prepared from the following components in parts by weight: 3 to 8 parts of streptomyces globisporus fermentation liquor, 4 to 7 parts of crab shell extract, 5 to 10 parts of limonene, 1 to 3 parts of beta-phellandrene, 2 to 5 parts of caryophyllene and 3 to 6 parts of citric acid. The method has the following beneficial effects: the disinfectant used in the disinfection method disclosed by the invention is highly toxic to pathogens, and a small amount of disinfectants can take a very good effect, the shrimp eggs are well tolerant to the disinfectant, the pathogens are killed without affecting the hatchability, the problems of low survival rate and poor quality in a hatching process is effectively solved, the disinfection step is simple, the operation is convenient, the practicability is high and good economic benefits are realized.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a method for disinfecting shrimp eggs that does not affect the hatching rate. Background technique [0002] In recent years, my country's shrimp farming industry has developed rapidly, and a large number of products are exported. The farming areas are all over my country's Jiangsu, Shanghai, Zhejiang, Fujian, Jiangxi, Guangdong, Hunan, Hubei, Sichuan, Hebei, Henan, Shandong and other places. However, due to the outbreak of shrimp diseases , Seriously damaged the shrimp farming industry and related industries. Studies have shown that pathogens can cause disease outbreaks by invading shrimp eggs and spreading rapidly in fry ponds. [0003] The prior art, such as the Chinese invention patent with the authorized announcement number CN 103960182 B, discloses a method of using shrimp eggs to culture in vitro to establish a family of white prawns. Soaking and disinfection in potass...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/13A01K61/59A01N63/04A01N63/02A01N27/00A01N37/36A01P1/00
CPCA01K61/13A01K61/59A01N27/00A01N37/36A01N63/10A01N63/30A01N2300/00Y02A40/81
Inventor 黄振远李喜莲顾志敏郭建林
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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