The invention provides an eye-clearing composition, eye drops containing the same and a preparation method of the eye drops. According to the eye-clearing composition provided by the invention, borneol liquid and borneol camphor extract contain active components including borneol, eucalyptol, camphor, bornyl acetate, alpha-pinene, limonene, beta-myrcene, alpha-terpilenol, linalool, beta-pinene andalpha-caryophyllene; the active components can be used for opening channels of eye skin, promoting effective components in folium ginkgo extract, lily bulb extract, herba menthae extract, yellow chrysanthemum extract and bilberry fruit extract to permeate into bottom-layer skin of eyes, promoting the metabolism of the skin, alleviating eye fatigue, removing free radicals and resisting wrinkles and resisting ageing. According to the preparation method provided by the invention, active components in borneol camphor branches and leaves are sufficiently extracted, active components including total flavonoids and ginkgolides in folium ginkgo are sufficiently extracted and an active compound, i.e., a phenolic acid glyceride glycoside compound, is sufficiently extracted; the preparation method provided by the invention has the advantages that the target active substance is sufficiently extracted and the extraction efficiency is high.