Lygus pratensis linnaeus host plant volatile matter and application thereof

A technology of Lygus and host plants, which is applied in the fields of application, plant growth regulator, botanical equipment and methods, etc., can solve the problems of hidden harm, increased harm of Lygus, and easy to be ignored, and achieve the effect of preventing disasters

Active Publication Date: 2018-06-29
TARIM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its hazards are hidden and easily overlooked, seriously affecting the normal development of plants, and seriously affecting the yield and quality of crops.
[0003] In recent years, the control of plant diseases and insect pests has mainly relied on chemical control, but with the large-scale planting of Bt cotton, the amount of chemical pesticides used to control Lepidoptera pests in cotton fields has gradually decreased, and the harm of forage liguds has increased.

Method used

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  • Lygus pratensis linnaeus host plant volatile matter and application thereof
  • Lygus pratensis linnaeus host plant volatile matter and application thereof
  • Lygus pratensis linnaeus host plant volatile matter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The volatiles of the host plants of Lygus graminis were extracted by solid-phase microextraction (SPME).

[0023] Freshly isolated plants were taken before the experiment, and the wound part was wrapped with a cotton ball soaked in water to prevent the emission of volatiles produced by the wound, and brought back to the laboratory quickly. The host plant plants brought back to the laboratory were put into a 500ml wide-mouth bottle, and the mouth of the bottle was plugged tightly with a silica gel stopper. Place the extraction head in the GC inlet at 250°C for 1 hour activation, fix the SPME handle with an iron stand, slowly push out the extraction head, insert it into a jar, and extract volatiles for 40 minutes.

[0024] The extracts were separated and determined by GC-MS technology. figure 1 The GC-MS detection patterns of volatiles of 7 kinds of host plants of Lygus forages, respectively. Combining computer retrieval technology to identify the isolated compounds, a ...

Embodiment 2

[0036] Determination of Electroantennary Potential (EAG) Response of Lygus Lygus to Host Plant Volatile Odor Compound Standards

[0037]Measuring method of antennal potential: Take a 6-10-day-old female adult Lygus stinkbug with a finger-shaped tube, and then put it on ice to freeze to faint. Put it under a stereomicroscope with the ventral side up, press the abdomen with an insect needle, and cut it down quickly along the middle of the front leg segment of the Lygus ligus with a double-sided blade. After cutting, observe whether the tissue membrane at the part where the head is separated The tissue fluid flows out. If there is no tissue fluid flow out, it indicates that the membrane is intact and can be tested further; if the tissue membrane is ruptured, it cannot be used and needs to be prepared again. Afterwards, cut off a small part of the tips of the two antennae, and then use the prepared reference electrode (the capillary is soaked with 0.9% NaCl solution by weight) to ...

Embodiment 3

[0044] Determination of Approaching Behavioral Responses of Lygus Lygus Adults to Standard Odor Samples of Host Plant Volatile Compounds

[0045] The Y-type olfactory instrument was used to measure the tendency behavior of Lygus forage to 9 kinds of host plant volatiles. The Y-shaped olfactory instrument was made of colorless transparent glass with an inner diameter of 3 cm. 60°. The base is connected with the release tube of the adult ligus bug, the two arms are corked tightly, and the odor source is connected with a glass tube. Use a pipette gun to draw 10uL of liquid paraffin containing a single-component pure product and drop it on a folded filter paper strip (0.5cm×5cm). After standing for 30s, put the filter paper strip into a conical flask as a source of odor. Liquid paraffin was used as solvent and control. Use the QC-1B atmospheric sampler as the air flow power system, the odor source is connected to a vacuum pump, and before the air flow enters the odor source, it ...

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Abstract

The invention discloses a lygus pratensis linnaeus host plant volatile matter, which includes any mixture of one or more of nonanal, farnesene, ALPHA-pinene, 1-caryophyllene and cis-3-hexenyl acetate.The invention also discloses application of the lygus pratensis linnaeus host plant volatile matter in preparing a lygus pratensis linnaeus attractant and preventing and controlling the lygus pratensis linnaeus. The lygus pratensis linnaeus host plant volatile matter is studied from the aspects of electrophysiology and behaviors, and it is proved that the nonanal, the farnesene, the ALPHA-pinene,1-caryophyllene and the cis-3-hexenyl acetate have the attracting action to the lygus pratensis linnaeus, wherein the attracting ratio of the nonanal reaches up to 66.7 percent. The lygus pratensis linnaeus host plant volatile matter provided by the invention is a potential candidate component of an approaching-avoidance agent or the attractant, can be used for designing a lygus pratensis linnaeus approach behavior regulating agent to be used for forecasting, preventing and controlling the lygus pratensis linnaeus, and thus effectively preventing the lygus pratensis linnaeus from causing disasters to agriculture and animal husbandry in China.

Description

technical field [0001] The invention relates to the technical field of plant disease and insect pest control, in particular to a volatile substance of a Lygus stinkbug host plant and an application thereof. Background technique [0002] Lygus pmtensis belongs to Hemiptera, Lygus pmtensis. Distributed in Inner Mongolia, Ningxia, Anhui, Hubei, Sichuan and other places. Harm corn, wheat, cotton, beans, vegetables and other crops. Adults and nymphs pierce and suck young shoots and young leaves and leaf juice. After the young tissues are damaged, dark brown spots appear at first, and then turn yellow and wither. Its hazards are hidden and easily overlooked, seriously affecting the normal development of plants, and seriously affecting the yield and quality of crops. [0003] In recent years, the control of plant diseases and insect pests has mainly relied on chemical control, but with the large-scale planting of Bt cotton, the amount of chemical pesticides used to control Lepid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N35/02A01N27/00A01N37/02A01P19/00
CPCA01N27/00A01N35/02A01N37/02
Inventor 冯宏祖王兰孙鹏
Owner TARIM UNIV
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