4 results about "Cell electrophysiology" patented technology

The invention relates to the technical field of model construction, in particular to a heart model construction method based on multiple physical fields and geometric multiple scales, which comprises the following steps of: performing segmentation processing and three-dimensional model reconstruction on a heart image to generate an initial heart geometric model; performing anisotropic attribute assignment on the initial heart geometric model based on a regularization algorithm to obtain a myocardial tissue structure; embedding a cell electrophysiological model into the computational grid of the myocardial tissue structure through parameter mapping to output a heart model with multi-scale geometric characteristics; an electrophysiological field equation, a mechanical field equation and a heart fluid dynamic field equation are constructed respectively, and coupling among multiple physical fields is achieved; determining whether the feature weight of the myocardial tissue structure texture needs to be increased or not; determining whether the transition function width of cell electrophysiological model parameter mapping needs to be increased or not; and determining a pressure feedback relaxation factor in the coupling iteration. The stability of heart model construction is improved.

The present application relates to the technical field of model construction, and particularly relates to a heart model construction method based on multi-physical field and geometric multi-scale, comprising: carrying out segmentation processing and three-dimensional model reconstruction on a heart image to generate an initial heart geometric model, carrying out anisotropic attribute assignment on the initial heart geometric model based on a regularization algorithm to obtain a myocardial tissue structure; embedding a cell electrophysiology model into a calculation grid of the myocardial tissue structure through parameter mapping to output a heart model with multi-scale geometric characteristics; respectively constructing an electrophysiology field equation, a mechanics field equation and a heart fluid dynamics field equation to realize coupling between the multi-physical fields; determining whether the feature weight of the myocardial tissue structure texture needs to be increased; determining whether the transition function width of the cell electrophysiology model parameter mapping needs to be increased; and determining a pressure feedback relaxation factor in the coupling iteration. The present application improves the stability of heart model construction.

The invention belongs to the technical field of signal detection, and relates to a three-dimensional integrated cell electrophysiology platform and a preparation method and application thereof.The three-dimensional integrated cell electrophysiology platform is composed of microelectrodes and a three-dimensional micro-scaffold, and the microelectrodes comprise a two-dimensional plane microelectrode and a three-dimensional microelectrode; wherein the microelectrode is used for applying stimulation to cells in a two-dimensional plane and a three-dimensional space and collecting electrophysiological signals; the three-dimensional micro-scaffold is used for simulating a three-dimensional microenvironment of normal physiological activities of cells; the average diameter of the two-dimensional planar microelectrode and the average diameter of the three-dimensional microelectrode are each independently 1 [mu] m or less. The three-dimensional integrated cell electrophysiology platform provided by the invention has the functions of high-precision processing, high structural design flexibility and high integration, and is suitable for various scenes such as cell culture, cell detection, cell excitation and the like.

An exemplary method and system is disclosed that facilitate the integration of multiplexed single-cell impedance cytometry in a high throughput format, which can be deployed upstream from microfluidic sample preparation and / or downstream to microfluidic cell separation. In exemplary method and system may employ impedance-based quantification of cell electrophysiology on the same microfluidic chip (i.e., “on-chip”) to provide distinguishing phenotypic information on the sample, without the need for additional sample handling, preparation or dilution steps as would be needed for other flow cytometry techniques.