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Novel glycoside hydrolase inhibitor preparation method

A technology of glycoside hydrolase and inhibitor, which is applied in the research and development field of microbial drugs, can solve the problems of complex extraction process and environmental pollution, and achieves the effects of high extraction yield, improved purity and high inhibitory activity.

Inactive Publication Date: 2009-02-04
MICROBIOLOGY INST OF SHAANXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The currently clinically used glycosidase inhibitor drug acarbose (commodity name: sugar-baking apple) is extracted from the fermentation metabolites of Actinomycetes mobilis, and its extraction process is complex and needs to be eluted with acid Liquid, easy to cause pollution to the environment (refer to patent 02136309.9)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Collect 1L of Streptomyces globisporus Waksman (purchased from China General Microorganism Culture Collection and Management Center, No. 4.187) fermentation broth, and heat up to 90°C for sterilization;

[0036] 2. Ultrafiltration through a tubular inorganic ceramic membrane with a membrane pore size of 0.1 μm (produced by Nanjing Jiusi High-Tech Co., Ltd.), the amount of water added is 1 times the amount of the raw material liquid, and the mycelium, soluble protein, and culture medium with a diameter greater than 0.1 μm are removed. base and pigment to obtain 1L of yellow transparent ultrafiltrate, and measure the α-glucosidase inhibitory activity and α-amylase inhibitory activity in the ultrafiltrate.

[0037] The determination method is as follows:

[0038] α-glucosidase inhibitor activity determination method (refer to Chen Haimin, Yan Xiaojun, structure-activity relationship of α-glucosidase inhibitors [J], Chinese Journal of Biochemistry and Molecular Biology, ...

Embodiment 2

[0057] 1. Collect 1L of Streptomyces globisporus Waksman (purchased from China General Microorganism Culture Collection and Management Center, No. 4.187) fermentation broth, and heat up to 90°C for sterilization;

[0058] 2. Ultrafiltration through a tubular inorganic ceramic membrane with a membrane pore size of 0.2 μm (produced by Nanjing Jiusi High-Tech Co., Ltd.), the amount of water added is 0.8 times the amount of the raw material liquid, and the mycelium, soluble protein, and culture medium with a diameter greater than 0.2 μm are removed. base and pigment to obtain 1.2L of yellow transparent ultrafiltrate, and measure the α-glucosidase inhibitory activity and α-amylase inhibitory activity in the ultrafiltrate according to the method of embodiment 1.

[0059] 3. 1.2L of ultrafiltrate is nanofiltered through an organic roll-type polyethersulfone membrane nanofiltration membrane (produced by Beijing Anshi Environmental Engineering Co., Ltd.) with a molecular weight cut-off ...

Embodiment 3

[0065] 1. Collect IL Streptomyces globisporus Waksman (purchased from China General Microorganism Culture Collection and Management Center, No. 4.187) fermentation broth, and heat up to 90°C for sterilization;

[0066] 2. Ultrafiltration through a tubular inorganic ceramic membrane with a membrane pore size of 0.2 μm (produced by Nanjing Jiusi High-Tech Co., Ltd.), the amount of water added is 0.8 times the amount of the raw material liquid, and the mycelium, soluble protein, and culture medium with a diameter greater than 0.2 μm are removed. base and pigment to obtain 1.2L of yellow transparent ultrafiltrate, and measure the α-glucosidase inhibitory activity and α-amylase inhibitory activity in the ultrafiltrate according to the method of embodiment 1.

[0067] 3. 1.2L of ultrafiltrate is nanofiltered through an organic roll-type polyethersulfone membrane nanofiltration membrane (produced by Beijing Anshi Environmental Engineering Co., Ltd.) with a molecular weight cut-off of ...

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Abstract

Belonging to the field of the research and development of microbial medicines, the present invention relates to a preparation method for extracting a novel glycoside hydrolase inhibitor from the fermentation liquor of Streptomyces globisporus. The method has the following characteristics: the fermentation liquor of Streptomyces globisporus is sterilized with high temperature, ultrafiltered and nanofiltered; the concentrated solution is absorbed and eluted by macroporous absorption resin; and after depressurization and concentration, the active component is frozen and dried, so that the glycoside hydrolase inhibitor is prepared. The method has the following advantages: the extraction technique is simple; the extraction process does not need acid and alkali; environment can be protected; and moreover, the extracted glycoside hydrolase inhibitor has inhibition activity on both pancreatic Alpha-amylase and Alpha-glucosaccharase and can be used for the development of blood sugar-decreasing products and weight-losing products.

Description

technical field [0001] The invention belongs to the field of research and development of microbial drugs, and in particular relates to a preparation method for extracting and preparing a novel glycoside hydrolase inhibitor from the fermentation liquid of the microorganism Streptomyces coccidioides. Background technique [0002] Diabetes mellitus is a group of clinical syndromes caused by genetic factors and environmental factors, and it is one of the diseases with the highest incidence in the world. Epidemiological studies have shown that among the two major blood glucose indicators, fasting blood glucose level and postprandial blood glucose level, postprandial blood glucose level is particularly important. It is an important factor for patients with impaired glucose tolerance (IGT) to develop type 2 diabetes. It is an important factor causing macrovascular complications and microvascular complications in diabetic patients, and these complications are an important reason for...

Claims

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Application Information

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IPC IPC(8): C12P1/04C12R1/465
Inventor 杨明琰张晓琦马瑜田稼
Owner MICROBIOLOGY INST OF SHAANXI
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