The invention relates to a preparation method of
animal glue for improving
hair loss. The preparation method comprises the following steps of (1) thoroughly cleaning fresh Qinghai-Tibet
plateau yak
skin having hair, and performing illumination through an
ultraviolet lamp so as to obtain illuminated yak
skin; (2)
cutting the illuminated yak
skin into blocks, performing extraction in boiling water,taking out the yak skin blocks after extraction, and
cutting through the yak skin with a multi-stab board; (3) putting the
cut-through yak skin in enzymatic
hydrolysate of 0.5-10% for enzymolysis, andperforming
filtration so as to obtain yak skin after enzymolysis and a first-time enzymolysis solution; (4) adding the yak skin after enzymolysis into water, and performing boiling so as to obtain filtrate; (5) adding
activated carbon to the filtrate, performing sufficient stirring, then performing standing, and performing
filtration so as to obtain filter residues; (6) putting the filter residues in enzymatic
hydrolysate of 0.5-8% for enzymolysis, and performing
filtration so as to obtain a second-time enzymolysis solution; (7) merging the first-time enzymolysis solution with the second-timeenzymolysis solution, performing boiling, and performing separation so as to obtain supernatant; and (8) filtrating the supernatant with a polyethersulfone membranes,
cutting off
keratin liquid of which the molecular weight is 40-75kDa and yak skin micromolecule
peptide of which the molecular weight is 5kDa or below, and performing
freeze drying so as to obtain yak glue. The method disclosed by the invention is simple and easy to implement.