Streptomyces strain and combined application thereof in prevention and treatment of tomato blight

A technology of Streptomyces and Streptomyces fissures, applied in the field of microorganisms, can solve the problems of rare reports on the biological control of tomato wilt pathogens

Active Publication Date: 2015-02-11
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Soil is an important place for actinomycetes to inhabit. Obtaining actinomycetes with high antibacterial activity from soil is an indispensable basic work for the research and devel

Method used

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  • Streptomyces strain and combined application thereof in prevention and treatment of tomato blight

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Embodiment 1

[0017] Embodiment 1: ( Streptomyces rimosus M527 and Streptomyces globisporus Isolation and screening of M927)

[0018] Follow these steps:

[0019] (1) Materials and culture medium: the materials used to isolate Streptomyces chamobilis M527 and Streptomyces coccoides M927 are soil samples collected from the saline-alkali land in the suburbs of Tai'an City, Shandong Province, and the culture medium used for the isolation of actinomycetes contains heavy Potassium chromate PDA medium (the formula is: weigh 200g potatoes, wash, peel and chop them, add 1L of distilled water to boil for half an hour, filter with gauze, add 20g of glucose and 20g of agar, fully dissolve and filter with gauze while hot , divided into Erlenmeyer flasks or glass test tubes, sterilized under high pressure at 121°C for 20 minutes; add potassium dichromate 20 μg mL when used -1 ; In the following experiments, the medium used for routine culture of actinomycetes was all configured with distilled wate...

Embodiment 2

[0028] Embodiment 2: ( Streptomyces rimosus M527 and Streptomyces globisporus Preparation method of M927 fermentation metabolite)

[0029] Follow these steps:

[0030] (1) Activation culture of strains: the stored Streptomyces rimosus M527 and Streptomyces globisporus M927 spore suspension (1×10 8cfu mL -1 ) into the potato dextrose liquid medium, cultivated for 48 hours at 28°C±1°C for fermentation;

[0031] (2) Fermentation culture: Fermentation medium contains 10g of soybean cake powder, 4g of corn flour, 20g of soluble starch, 5g of glucose, 5g of peptone, and CoCl 2 0.02g, beef extract 5g, CaCO 3 5g, 5g of yeast extract, 50mL of fermentation medium per 300mL triangular bottle; after preparation, sterilize at 121°C for 20min, and use potato glucose liquid medium to culture Streptomyces rimosus M527 and Streptomyces globisporus M927 was inserted into the fermentation medium at 5% (v / v) respectively, under the condition of 28°C±1°C, the shaker speed was 1...

Embodiment 3

[0034] Embodiment 3: ( Streptomyces rimosus M527 and Streptomyces globisporus Inhibitory effect of metabolites of M927 on Fusarium wilt of tomato)

[0035] (1) Accurately weigh Streptomyces rimosus M527 and Streptomyces globisporus Add 0.1g of M927 metabolites, add 10mL of sterile water, dissolve fully by ultrasonic vibration, and prepare mother solutions with a final concentration of 10 g / L;

[0036] (2) Determination of antibacterial activity: Take the above mother liquor and dilute it into solutions with a concentration of 1g / L and 0.5g / L respectively, put 1mL of each concentration solution in a sterile petri dish, and rapidly mix with 9mL of PDA medium cooled to 50°C mix ( Streptomyces rimosus M527 and Streptomyces globisporus The final concentrations of M927 metabolites are respectively 0.1g / L and 0.05g / L), and after cooling, put a 4mm tomato fusarium bacterium bacterium cake on each culture medium plane respectively, and the bacterium cake is connecte...

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Abstract

The invention discloses a streptomyces strain and a combined application thereof in the prevention and treatment of tomato blight and relates to the technical field of microbes. According to the streptomyces strain and the combined application thereof, actinomycetes are obtained through collecting 10-15cm deep-layer soil samples from saline-alkaline land of a suburban district of Tai'an in Shandong and separating by adopting an actinomycete separation technology, and are named streptomyces rimosus M527 and streptomyces globisporus M927 through microbial taxonomic identification, S. rimosus M527 has been collected in CCTCC (China Center for Type Culture Collection) on June 19, 2013 and has the collection number of CCTCC NO: M 2013270, and S. globisporus M927 has been collected in CCTCC on December 25, 2013 and has the collection number of CCTCC NO: M 2013709. Both strains can be fermented to generate active substances which have relatively strong antagonism on Fusarium oxysporum f. sp. Lycopersici Snyder et Hansen and can be used for preventing and treating plant diseases, such as tomato blight. When fermentation products of the two strains are mixed according to the weight percent of (4: 1) to (7: 3), the biological prevention effect is particularly remarkable compared with that when the two kinds of metabolic products are used respectively as single preparations.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and relates to the application of metabolites of two strains of biocontrol actinomycetes, Streptomyces fissures M527 and Streptomyces coccidioides M927, in the prevention and treatment of tomato wilt. technical background [0002] tomato( Lycopersicon esculentum Mill) is a worldwide vegetable crop and one of the main vegetables grown in my country. Tomato wilt is one of the main diseases of tomato. The disease is highly contagious and seriously affects the yield and quality of tomato. [0003] Fusarium oxysporum specialization is the pathogenic bacteria of tomato fusarium wilt Fusarium oxysporum f. sp. Lycopersici Snyder et Hansen, Tomato Fusarium wilt is highly specialized and only harms tomatoes. The source of the disease is the tomato wilt pathogen of the subphylum Ignomonycota and the genus Fusarium. The fungus survives the winter in the soil and spreads with rain, irrigation wat...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/59C12R1/465
CPCA01N63/10C12N1/205C12R2001/465C12R2001/59
Inventor 马正俞晓平路丹丹罗帅边亚琳王正亮申屠旭萍
Owner CHINA JILIANG UNIV
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