Compound microbial source pesticide for effectively preventing and treating diseases of oil-tea trees and application method

A technology of compound microorganisms and camellia oleifera, applied in the directions of botanical equipment and methods, applications, biocides, etc., can solve the problems of polluted environment, unsatisfactory effect, chemical control restrictions, etc., and achieves high activity, good control effect, and good control effect. The effect of the control effect

Inactive Publication Date: 2014-11-26
HAINAN KEDA FORESTRY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, thiophanate and other chemical pesticides were mostly sprayed against this disease, but the effect was not satisfactory. Moreover, due to drug residues and environmental pollution, chemical control has become more and more restricted.

Method used

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  • Compound microbial source pesticide for effectively preventing and treating diseases of oil-tea trees and application method
  • Compound microbial source pesticide for effectively preventing and treating diseases of oil-tea trees and application method
  • Compound microbial source pesticide for effectively preventing and treating diseases of oil-tea trees and application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Composite bacteria fermentation process optimization

[0031]Taking the pathogenic bacteria of Camellia oleifera as the indicator strain, the bacteriostatic rate and the dry weight of the bacteria as the main screening indicators, the best fermentation medium was screened out: glucose 15g, soybean powder 10g, NaCl 5g, yeast extract 1g, CaCO 3 1g, distilled water 1000mL, pH7.0. The best fermentation conditions are: inoculation amount 10%, liquid volume 100mL, inoculation volume ratio of actinomycetes and bacterial seed liquid is 3:1, actinomycete seed liquid is inoculated first, and the inoculation interval is 5 days, and then bacterial seeds are inoculated solution, the culture conditions were 28°C, 160r / min, and the total shaking culture time was 7 days. Under these conditions, the dry weight of the bacteria was 1.6354g, and the bacteriostasis rate was 97.19%. After adopting this optimized fermentation process, the bacteriostasis rate and dry weight of t...

Embodiment 2

[0036] Embodiment 2 Composite microbial source pesticide development

[0037] According to biocompatibility test, the selected carrier is kaolin, the wetting agent is sodium lauryl sulfate, the dispersant is sodium lignosulfonate, the binder is polyethylene glycol, and the disintegrating agent is ammonium sulfate. The compound bacteria fermentation liquid and kaolin were mixed according to the ratio of material to liquid of 7:1 to make raw powder for later use. The formula optimization results show that the raw powder is 40%, the wetting agent sodium lauryl sulfate is 4%, the dispersant is sodium lignosulfonate 5%, the disintegrating agent is ammonium sulfate 4%, the binder polyethylene glycol is 2%, The amount of water added is controlled between 16-20%, and the carrier kaolin supplements 100%, which is the optimal formula of the bacterial agent. The wettability, dispersibility, suspension, disintegration time, and granulation rate of the composite microbial source pesticide...

Embodiment 4

[0066] Example 4 Significance level analysis between different groups

[0067] The significance level analysis was carried out on the antibacterial activity and the dry weight of the bacteria of the single antagonistic actinomycete Streptomyces coccidioides subsp. The analysis results are shown in Table 3.

[0068] Table 3 Significance level analysis among different groups

[0069]

（1）

（2）

（3）

（4）

Bacteriostatic rate (%)

91.3±2.05b

78.84±1.3d

97.19±2.04a

87.46±1.33c

Bacterial dry weight (g)

0.9835±0.0012c

1.131±0.002b

1.6354±0.0102a

0.2161±0.0002d

[0070] Note: Duncan’s test is used, the same letter after the value in the same column indicates no significant difference (P>0.05), and all different letters indicate significant difference (P<0.05).

[0071] In Table 3:

[0072] (1) Streptomyces coccidioides subspecies CSUFTAC0906002 fermentation broth;

[0073] (2) Streptomyces coccidioides subsp...

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Abstract

The invention discloses a compound microbial source pesticide for effectively preventing and treating diseases of oil-tea trees and an application method, and in particular relates to a microbial pesticide prepared by actinomycetes globisporus and streptomyces globisporus subspecies CSUFTAC0906002 (preserving number: CCTCC NO: M2011012) and bacterial bacillus brevis Z26 (preserving number: CCTCC NO: M2011013) compounded with other assistants. The invention belongs to the technical field of forest protection. The compound microbial source pesticide can effectively inhibit growth and reproduction of main pathogenic bacteria of the oil-tea trees, and meanwhile, the main components are good in safety to the environment and have good development application prospect.

Description

technical field [0001] The invention belongs to the field of forest protection, and in particular relates to a compound microbial source pesticide for preventing and treating main diseases of camellia oleifera forest and its application method. Background technique [0002] Camellia oleifera (Camellia oleifera) belongs to the evergreen small tree of the family Theaceae, and is mainly produced in my country. It is one of the four major woody oil tree species in the world. Camellia oleifera is widely distributed in my country, from the southeastern coastal hills to the western Yunnan-Guizhou Plateau. Camellia oleifera has high yield, long lifespan, strong adaptability, and no strict requirements on soil conditions. It is cultivated in large areas in hills and mountains in 16 provinces south of Qinling and Huaihe Rivers. Camellia oleifera bears fruit earlier, and it generally takes only 3-5 years from planting to flowering and fruiting, but the fruiting period can reach 50-60 ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01P3/00A01P1/00
Inventor 周国英何苑皞刘君昂李河赵莹王瑞芹孟庆敏
Owner HAINAN KEDA FORESTRY CO LTD
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