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Functional Components and Application of Rue Essential Oil

A technology of volatile oil and rue, which is applied in the field of application of rue volatile oil in inhibiting plant pathogenic bacteria, and can solve problems such as pollution and affecting the quality of edible fungi

Active Publication Date: 2021-10-12
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these miscellaneous bacteria grow rapidly, and if the prevention is not timely, the quality of edible fungi will be seriously affected
Although chemically synthesized drugs to kill plant pathogenic fungi play an irreplaceable role in the prevention and control of plant diseases, people pay more and more attention to the toxicity of pesticides and the pollution of pesticides to the environment. Some bactericidal drugs with high toxicity and long residue Drug varieties, such as organochloromercury preparations (aldrin, 666), etc. have been banned in succession

Method used

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  • Functional Components and Application of Rue Essential Oil
  • Functional Components and Application of Rue Essential Oil
  • Functional Components and Application of Rue Essential Oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Pathogenic fungi tested: Aspergillus flavus, Rhizopus stolonifer, Mucor racemosus, Trichoderma viride, Colletotrichum lentis, Fusarium oxysporum Fusarium oxysporum and Phytophythora infestans.

[0043]Plants tested: rue (Cymbopogon distans), that is, a plant that is sandwiched in books to make it emit fragrance.

[0044] The preparation method of rue volatile oil is: put rue 250 grams of rue plant into a round bottom flask, add 350 g of distilled water, adopt steam distillation to collect volatile oil, the oil-water mixture collected is extracted with n-hexane, then with anhydrous sulfuric acid Sodium for drying, and finally freeze-drying with a freeze dryer to obtain volatile oil, which was stored in a 4°C refrigerator.

[0045] experimental method:

[0046] The inhibitory effect of rue volatile oil on the mycelial growth of plant pathogenic fungi was determined by the growth rate method in toxic media.

[0047] Weigh 8 mg of rue volatile oil and dissolve it with 10...

Embodiment 2

[0066] Tested fungi: Aspergillus flavus, Rhizopus stolonife, Mucor racemosus, Trichoderma viride, Colletotrichum lentis, Fusarium oxysporum (Fusarium oxysporum) and potato infestans (Phytophythora infestans).

[0067] The method for detecting the antibacterial activity of plant volatile oil and main compounds is as follows:

[0068] The colony diameter of the mycelia of the seven plant pathogenic bacteria under each treatment was tested in a criss-cross manner, and the smaller the value of the diameter, the stronger the inhibitory activity.

[0069] The preparation process of the compound 1-4 for testing is as follows, the obtained rue grass volatile oil is passed through silica gel column chromatography, according to normal hexane and ethyl acetate (volume ratio is successively 100:0, 50:1, 20:1, 10:1, 8:1, 4:1, 2:1, 1:1, 0:1) for gradient elution to obtain Fr.1-Fr.9 fractions respectively.

[0070] Part FR.2 was chromatographed on a silica gel column (petroleum ether and d...

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Abstract

The invention provides the application of rue volatile oil in inhibiting phytopathogenic bacteria or preparing plant-derived fungicides; the phytopathogenic bacteria are Aspergillus flavus, Rhizopus glucoides, Mucor racemosa, Trichoderma viridans, C. Fusarium and / or P. infestans. The applicant proves by activity inhibition experiment that the volatile oil of rue grass has a strong inhibitory rate to these four kinds of fungi of Rhizopus glucuronidum, Mucor racemosa, Trichoderma viride and Trichosporium lentil; The main chemical components were also tested for their inhibitory activity against these 7 fungi. The results showed that the same compound exhibited different degrees of inhibitory effects on different pathogenic bacteria, and different compounds showed different inhibitory effects on the same pathogenic bacteria. Some of the compounds showed Obvious inhibitory effect, so the essential oil and main chemical components of rue can be used as very promising botanical fungicides.

Description

technical field [0001] The present invention relates to the active components of rue volatile oil and application thereof, in particular to the application of rue volatile oil in inhibiting plant pathogenic bacteria. Background technique [0002] In the planting industry of my country's edible fungi, it is the sixth largest product after grain, cotton, oil, fruit, and vegetables. It is a pillar industry and a dominant industry in agriculture and rural economy. Some miscellaneous bacteria caused a decrease in production, and some miscellaneous bacteria produced toxins, which caused our country to encounter a crisis in import and export. Common fungal miscellaneous bacteria in the reagent generation of edible fungi mainly include: Trichoderma viride, Neurospora, Mucor, Aspergillus, Rhizopus niger, yeast and so on. Most of these miscellaneous bacteria grow rapidly, and if the prevention is not timely, the quality of edible fungi will be seriously affected. Although chemically ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/44A01P3/00
CPCA01N65/00A01N65/44
Inventor 宋秋艳任婷高坤陈建军王为王森李世才
Owner LANZHOU UNIVERSITY
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