Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus reuteri ZJUIDS09 for reducing blood pressure and application thereof

A Lactobacillus reuteri, blood pressure lowering technology, applied in the direction of Lactobacillus, application, bacteria used in food preparation, etc., can solve the problem that the blood pressure lowering effect of GMNL-263 live bacteria has not been proved, and achieve a high ACE inhibition rate. Effect

Active Publication Date: 2021-09-24
ZHEJIANG UNIV
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This scheme reflects the application effect of the composition of Lactobacillus reuteri strain GMNL-263 and Bifidobacterium in the population, and the product used in this scheme is the inactivated product of Lactobacillus reuteri strain GMNL-263 , works by inhibiting a pro-inflammatory cytokine IL 1β and promoting a bifidobacterium, but it has not been proved that GMNL-263 live bacteria have a good antihypertensive effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus reuteri ZJUIDS09 for reducing blood pressure and application thereof
  • Lactobacillus reuteri ZJUIDS09 for reducing blood pressure and application thereof
  • Lactobacillus reuteri ZJUIDS09 for reducing blood pressure and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, screening and identification of Lactobacillus reuteri ZJUIDS09:

[0035] 1. Screening of Lactic Acid Bacteria

[0036] 1.1 Sample source

[0037] The bacterial strain used in the present invention is derived from milk fans made by farmers in Yunnan. A total of 15 samples were collected, which were immediately refrigerated and mailed to the laboratory for isolation and purification of the strains.

[0038] 1.2 Isolation and purification of strains

[0039] About 5 g of fresh samples were collected with sterile tubes and immediately sent to the laboratory for strain isolation. Take 1 g of the sample and put it into 9 mL of MRS liquid medium, vortex and mix it, and enrich and culture it at 37°C for 48 hours; then absorb 1 mL of the enriched solution in a super-clean bench, perform ten-fold gradient dilution with sterile normal saline, and select 10 -6 、10 -7 、10 -8 For three dilution gradients, 100 μL of bacterial solution was taken from each gradient ...

Embodiment 2

[0050] Example 2, Confirmation of the ability of Lactobacillus reuteri ZJUIDS09 to lower blood pressure

[0051] 1. ACE inhibition rate

[0052] Preparation of strain fermentation broth

[0053] Streak the strain ZJUID S09 stored in the glycerol tube to activate it 2-3 times on the MRS agar plate, then pick a single colony and expand it in the MRS liquid medium for 18 hours at 37°C, so that the concentration of the bacterial solution reaches about 10 8 About CFU / mL, as a culture medium. The culture solution was inoculated into sterilized reconstituted skim milk (11%, w / v) at an inoculum size of 2% (volume %), cultured at 37°C for 48h, taken out, and placed in a refrigerator at 4°C overnight. Take the fermented milk out of the refrigerator, adjust the pH to 4.6, centrifuge at 8000g for 10min to save the supernatant, adjust the pH to 8.2, centrifuge at 8000g for 10min, and use the collected supernatant as the fermentation supernatant of strain ZJUID S09.

[0054] The steriliz...

Embodiment 3

[0078] Embodiment 3, confirmation of the antioxidant capacity of Lactobacillus reuteri ZJUIDS09

[0079] The culture solution prepared in Example 2 was centrifuged at 8000r / min for 5min at 4°C to collect the bacteria, washed twice with phosphate buffer (pH 6.8), and suspended in the MRS liquid medium to make the live bacteria number corrected to about 10 8 CFU / mL, bacterial suspension;

[0080] The bacterial suspension and the fermentation supernatant prepared in Example 2 were used as samples. At the same time, Lactobacillus rhamnosus ATCC53103 was used as a positive control strain to measure the antioxidant activity.

[0081] 1. Total antioxidant capacity (FRAP method)

[0082] The determination method of total antioxidant capacity was slightly modified according to the method of Giuberti et al. Add 150 μL TPTZ working solution (0.3M acetic acid-sodium acetate buffer solution, 20mM ferric chloride solution, 10mM TPTZ buffer solution, mix in V:V:V=10:1:1, and prepare imme...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of food microorganisms, and particularly discloses Lactobacillus reuteri ZJUIDS09, the preservation number of which is CGMCC NO. 22843. The invention also provides application of the Lactobacillus reuteri ZJUIDS09 in preparing a product with a function of reducing blood pressure.

Description

technical field [0001] The invention relates to the technical field of food microorganisms, in particular to Lactobacillus reuteri ZJUIDS09 with the function of lowering blood pressure and its application. Background technique [0002] At present, hypertension has become a metabolic disease with a large number of people in China and the world. Hypertension has a serious impact on human health and can be accompanied by a clinical syndrome of functional or organic damage to the heart, brain, kidneys and other organs. Hypertension is the most common chronic disease and the main risk factor for cardiovascular and cerebrovascular diseases. Angiotensin converting enzyme (Angiotensinconverting enzyme, ACE) plays an important role in blood pressure. It can convert angiotensin I into angiotensin II, which has the function of constricting blood vessels. It can also hydrolyze bradykinin, which has a vasodilation effect. . Antihypertensive peptides in fermented milk can cause vasodil...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P9/12A23L33/135A23L2/38A23C9/123A23L13/70A23C9/16A23C9/152A23K10/18C12R1/225
CPCA61K35/747A61P9/12A23L33/135A23L2/382A23C9/1234A23L13/74A23C9/16A23C9/152A23K10/18A23V2002/00A23V2400/173A23V2250/628A23V2250/51082A23V2300/26A23V2200/326A23V2200/30A23V2250/61
Inventor 任大喜宋小玲高冀婷曹菲薇
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com