Fungus with strong weed suppression effect screened from passion fruit rhizosphere soil

A technology for rhizosphere soil and herb-inhibiting fungi, applied in fungi, applications, herbicides and algicides, etc., can solve the problems of soil and groundwater pollution, increase the residue of harmful substances, etc., and achieve good environmental compatibility and environmental protection. safe effect

Inactive Publication Date: 2017-09-15
FUJIAN AGRI & FORESTRY UNIV
View PDF3 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term, large-scale, non-selective application of chemical herbicides can lead to soil and groundwater pollution, and wee

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fungus with strong weed suppression effect screened from passion fruit rhizosphere soil
  • Fungus with strong weed suppression effect screened from passion fruit rhizosphere soil
  • Fungus with strong weed suppression effect screened from passion fruit rhizosphere soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Biological test of the herb-inhibiting ability of the fermentation broth of the strain

[0023] Put a piece of filter paper in the tissue culture bottle respectively, take 5 mL of the fermented liquid diluted 50 times and add it to the tissue culture bottle, sow 5 pieces of lettuce that has just turned white in each tissue culture bottle, and distilled water as a control, and put them They were cultured in a constant temperature incubator at 28°C, and each experiment was repeated three times. The root length and plant height of lettuce were measured after 3 days of cultivation. The inhibition rate is calculated according to the formula: IR = (1- TR / CK ) × 100%. in, IR : Inhibition rate (Inhibition rate); TR : Treatment (Treatment); CK : Control.

[0024] Then we took barnyardgrass and wolf's tail grass as the experimental objects to test the ability of the fermentation broth to inhibit weeds. With barnyardgrass as the experimental object, the fermentation l...

Embodiment 2

[0028] Example 2 strain identification

[0029] 1. Morphological identification

[0030] Morphological observation of colonies

[0031] In the middle of the PDA solid medium plate, the fungus cake punched with a hole puncher with a diameter of 0.8 cm was inserted, continuously cultured at 28°C for 7 days, and the morphological characteristics such as texture and color pattern of the edge and surface of the colony were observed every day.

[0032] Microscopic Morphological Observation

[0033] Use a dissecting needle to pick out a little colony from the colony cultured on the PDA solid medium plate for 3 days, put it on a glass slide dripped with sterile water in the middle, then use two dissecting needles to disperse the colony as much as possible, and cover it with a cover glass Blot the water around the slide with absorbent paper, and observe the conidiophores and spores under an optical microscope.

[0034] The morphological identification was based on the characteristic...

Embodiment 3

[0043] Example 3 Determination of Phenolic Acids in Strain Fermentation Broth

[0044] Take 100 mL of fermentation broth, adjust the pH to 4.00 with phosphoric acid, then add 8 g of NaCl, and dissolve the solution for use. Take the CleanertPEP solid-phase extraction column and activate it by soaking and rinsing with distilled water-methanol-distilled water (8 mL-8 mL-6 mL) respectively, and then remove the residual methanol for later use. Add the treated fermentation broth to the activated PEP solid-phase extraction column. When the fermentation broth has run out, add 2mL of distilled water to rinse, then add 10mL of methanol to elute, and collect the eluate and freeze-dry it. . After dissolving with 1mL of methanol (chromatographically pure), use a 0.22μmL filter membrane to filter into the sample bottle, which is the sample of the fermentation broth.

[0045] Seven phenolic acids including protocatechuic acid, ferulic acid, cinnamic acid, vanillic acid, salicylic acid and ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of microbial herbicides and particularly relates to weed suppression fungus screened from passion fruit rhizosphere soil. Lettuce (compositae), barnyard grass (gramineae) and lysimachia barystachys (gramineae) are taken as receptors separately, biological test is carried out on the weed suppression ability of a fungus strain fermentation liquid, and the condition that the weed suppression fungus has a very high inhibition rate on root length and plant height of two receptors is found out. The condition that protocatechuic acid, p-hydroxybenzoic acid, vanillic acid, salicylic acid and cinnamic acid contain phenolic acid with a weed suppression effect is detected in the fermentation liquid. The strain is named FJ-01-BXG-08. The analysis and identification results on the morphology and ITS sequence of the strain show that the strain is one of Aspergillus sydowii (aspergillus sydowii), and strong weed suppression fungus aspergillus sydowii (Aspergillus sydowii) is screened from eucalyptus soil for the first time.

Description

technical field [0001] The invention belongs to the field of crop disease prevention and control, and in particular relates to a fungus with strong weed-inhibiting effect screened from passion fruit rhizosphere soil. Background technique [0002] passion fruit( Passiflora edulis ) has another name called passion fruit and egg fruit. It is a vine plant planted in the tropics and subtropics. Its juice has the functions of helping digestion and nourishing, and is very popular among people. It is estimated that the current planting area of ​​passion fruit in the world has reached more than 20,000 hectares. However, due to the increasing popularity of passion fruit, the market demand is constantly increasing, and the planting area of ​​passion fruit is also increasing. According to statistics, in the early 1980s, the world's demand for passion fruit exceeded 250,000 tons, but the production volume was only 120,000 tons, and the demand was far greater than the supply. In my coun...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/14A01N63/04A01P13/02C12R1/66
CPCA01N63/30C12N1/145C12R2001/66
Inventor 张奇李立陈尧杨小燕李家玉何海斌
Owner FUJIAN AGRI & FORESTRY UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap