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A fungus with strong herbicidal effect screened from passion fruit rhizosphere soil

A rhizosphere soil and anti-grass fungus technology, applied in the direction of fungi, application, herbicides and algicides, etc., can solve the problems of soil and groundwater pollution, increase the residue of harmful substances, etc., and achieve good environmental compatibility and environmental protection. safe effect

Inactive Publication Date: 2020-03-17
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term, large-scale, non-selective application of chemical herbicides can lead to soil and groundwater pollution, and weeds will also develop resistance
High concentrations of chemical herbicides can also increase the residue of harmful substances in crops

Method used

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  • A fungus with strong herbicidal effect screened from passion fruit rhizosphere soil
  • A fungus with strong herbicidal effect screened from passion fruit rhizosphere soil
  • A fungus with strong herbicidal effect screened from passion fruit rhizosphere soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Biological test of the herb-inhibiting ability of the fermentation broth of the strain

[0023] Put a piece of filter paper in the tissue culture bottle respectively, take 5 mL of the fermented liquid diluted 50 times and add it to the tissue culture bottle, sow 5 pieces of lettuce that has just turned white in each tissue culture bottle, and distilled water as a control, and put them They were cultured in a constant temperature incubator at 28°C, and each experiment was repeated three times. The root length and plant height of lettuce were measured after 3 days of cultivation. The inhibition rate is calculated according to the formula: IR = (1- TR / CK ) × 100%. in, IR : Inhibition rate (Inhibition rate); TR : Treatment (Treatment); CK : Control.

[0024] Then we took barnyardgrass and wolf's tail grass as the experimental objects to test the ability of the fermentation broth to inhibit weeds. With barnyardgrass as the experimental object, the fermentation l...

Embodiment 2

[0028] Example 2 strain identification

[0029] 1. Morphological identification

[0030] Morphological observation of colonies

[0031] In the middle of the PDA solid medium plate, the fungus cake punched with a hole puncher with a diameter of 0.8 cm was inserted, continuously cultured at 28°C for 7 days, and the morphological characteristics such as texture and color pattern of the edge and surface of the colony were observed every day.

[0032] Microscopic Morphological Observation

[0033] Use a dissecting needle to pick out a little colony from the colony cultured on the PDA solid medium plate for 3 days, put it on a glass slide dripped with sterile water in the middle, then use two dissecting needles to disperse the colony as much as possible, and cover it with a cover glass Blot the water around the slide with absorbent paper, and observe the conidiophores and spores under an optical microscope.

[0034] The morphological identification was based on the characteristic...

Embodiment 3

[0043] Example 3 Determination of Phenolic Acids in Strain Fermentation Broth

[0044] Take 100 mL of fermentation broth, adjust the pH to 4.00 with phosphoric acid, then add 8 g of NaCl, and dissolve the solution for use. Take the Cleanert PEP solid phase extraction column and activate it by soaking and rinsing with distilled water-methanol-distilled water (8 mL - 8 mL - 6 mL) respectively, and then remove the residual methanol for later use. Add the treated fermentation broth to the activated PEP solid-phase extraction column. When the fermentation broth has run out, add 2mL of distilled water to rinse, then add 10mL of methanol to elute, and collect the eluate and freeze-dry it. . After dissolving with 1mL of methanol (chromatographically pure), use a 0.22μmL filter membrane to filter into the sample bottle, which is the sample of the fermentation broth.

[0045] Seven phenolic acids including protocatechuic acid, ferulic acid, cinnamic acid, vanillic acid, salicylic acid...

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Abstract

The invention belongs to the field of microbial herbicides, in particular to a herb-inhibiting fungus screened from passion fruit soil. Using lettuce (compositae), barnyardgrass (poaceae) and pennisetum (poaceae) as receptors, biological tests were carried out on the herb-inhibiting ability of the fermentation broth of the fungus strain, and it was found that the root length of the two receptors and plant height have a strong inhibitory rate. Protocatechuic acid, p-hydroxybenzoic acid, vanillic acid, salicylic acid and cinnamic acid were detected in the fermentation broth as phenolic acids with herbicidal effects. And this bacterial strain is named as FJ‑01‑BXG‑08. The identification results of the strain's morphology and ITS sequence analysis showed that the strain was Aspergillus sydowii (Aspergillus spp.), a member of Aspergillus spp., for the first time to screen strong herbaceous fungus Aspergillus spp. (Aspergillus spp. Aspergillus sydowii ).

Description

technical field [0001] The invention belongs to the field of crop disease prevention and control, and in particular relates to a fungus with strong weed-inhibiting effect screened from passion fruit rhizosphere soil. Background technique [0002] passion fruit( Passiflora edulis ) has another name called passion fruit and egg fruit. It is a vine plant planted in the tropics and subtropics. Its juice has the functions of helping digestion and nourishing, and is very popular among people. It is estimated that the current planting area of ​​passion fruit in the world has reached more than 20,000 hectares. However, due to the increasing popularity of passion fruit, the market demand is constantly increasing, and the planting area of ​​passion fruit is also increasing. According to statistics, in the early 1980s, the world's demand for passion fruit exceeded 250,000 tons, but the production volume was only 120,000 tons, and the demand was far greater than the supply. In my coun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/34A01P13/02C12R1/66
CPCA01N63/30C12N1/145C12R2001/66
Inventor 张奇李立陈尧杨小燕李家玉何海斌
Owner FUJIAN AGRI & FORESTRY UNIV
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