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Litchi rind total-phenol extract mainly comprising polymer polyphenol and application of extract

A technology of litchi peel and extract, applied in the field of total phenolic extract of litchi peel, can solve the problems of poor inhibitory activity of polyphenols, restricted wide use and the like, and achieve the effects of broadening value, making full use and reducing production cost.

Active Publication Date: 2018-10-23
GUANGZHOU XIAOJING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Litchi polyphenols are distributed in litchi peel, leaves, and pulp. There have been a lot of reports on the extraction of polyphenols from litchi peel and pulp, but the polyphenols extracted by traditional extraction methods have poor inhibitory activity on tyrosinase, which greatly limits the wide range use

Method used

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  • Litchi rind total-phenol extract mainly comprising polymer polyphenol and application of extract
  • Litchi rind total-phenol extract mainly comprising polymer polyphenol and application of extract
  • Litchi rind total-phenol extract mainly comprising polymer polyphenol and application of extract

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Embodiment 1

[0044] Extract fresh lychee peel and 80% ethanol aqueous solution at 23°C for 2 times, soak for 8 days each time, filter, and combine the filtrate; centrifuge the filtrate at 3500rpm for 10min, collect the supernatant, and obtain the medicinal solution; The medicinal solution is purified by column chromatography through XDA-7 macroporous resin, and eluted with a 60% ethanol aqueous solution to obtain an extract eluate; the conditions for the column chromatography purification are: the mass concentration of the sample solution is 3.5 mg / mL, the pH value of the medicinal solution is 4.5, and the sample loading rate is 4BV / h; the flow rate of the eluent is 6BV / h; Concentrate under reduced pressure at a water temperature of 15°C, spray-dry the obtained reflux liquid, feed rate 51mL / min, inlet air temperature 160°C, exhaust air temperature 60°C, feed liquid concentration 22%, atomizer speed 21000r / min to obtain the total phenolic extract of litchi bark mainly composed of polymer p...

Embodiment 2

[0047] Extract fresh litchi peel and 80% ethanol aqueous solution at 27°C for 2 times, soak for 8 days each time, filter, and combine the filtrate; centrifuge the filtrate at 3500rpm for 10min, collect the supernatant, and obtain the medicinal solution; The medicinal solution is purified by column chromatography through XDA-7 macroporous resin, and eluted with a volume fraction of 60% ethanol aqueous solution to obtain an extract eluate; the conditions for column chromatography purification are: the mass concentration of the sample solution is 4.5 mg / mL, the pH value of the medicinal solution is 3.5, and the sample loading rate is 2BV / h; the flow rate of the eluent is 3BV / h; Concentrate under reduced pressure at a water temperature of 0°C, spray-dry the obtained reflux liquid, feed rate 51mL / min, inlet air temperature 160°C, exhaust air temperature 60°C, feed liquid concentration 22%, atomizer speed 21000r / min to obtain the total phenolic extract of litchi bark mainly compose...

Embodiment 3

[0050] Extract fresh lychee peel and 80% ethanol aqueous solution at 25°C for 2 times, soak for 7 days each time, filter, and combine the filtrate; centrifuge the filtrate at 3500rpm for 10min, collect the supernatant, and obtain the medicinal solution; The medicinal solution is purified by column chromatography through XDA-7 macroporous resin, and eluted with a 60% ethanol aqueous solution to obtain an extract eluate; the conditions for the column chromatography purification are: the mass concentration of the sample solution is 1.0 mg / mL, the pH value of the liquid medicine is 2.5, and the sample loading rate is 5BV / h; the flow rate of the eluate is 8BV / h; Concentrate under reduced pressure at a temperature of 30°C, and spray dry the obtained reflux liquid. min, the total phenolic extract of litchi bark mainly composed of polymer polyphenols was obtained.

[0051] The Folin-phenol colorimetric method was used for determination, and the measured polyphenol content was 76.8%. ...

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Abstract

The invention provides a litchi rind total-phenol extract mainly comprising polymer polyphenol and an application of the extract, and belongs to the field of natural-occurring substance extraction. Apreparation method of the litchi rind total-phenol extract includes the steps: soaking litchi rinds in ethanol aqueous solution with the volume concentration of 80% at the temperature of 23-27 DEG C,and performing solid-liquid separation on acquired soak solution to obtain liquid medicines; purifying the liquid medicines by a column chromatography, adsorbing extracted polar active substances on achromatographic column, eluting the polar active substances by the aid of ethanol aqueous solution with the volume concentration of 60%, enabling parts of polar active substances to be eluted, and concentrating and spray-drying acquired eluting liquid to prepare the litchi rind total-phenol extract mainly comprising the polymer polyphenol. The litchi rind total-phenol extract mainly comprising the polymer polyphenol has a strong inhibition effect on tyrosinase and is applied to whitening cosmetics or preservation of fruits and vegetables.

Description

technical field [0001] The invention belongs to the field of natural substance extraction, and in particular relates to a total phenolic extract of litchi bark mainly composed of polymer polyphenols and an application thereof. Background technique [0002] Whitening and freckle removal has always been the pursuit of people's health and beauty. Skin color is largely determined by the production, migration and distribution of melanin. At present, research on whitening and freckle removal focuses on blocking the formation of melanin. Melanin is a high-molecular biological pigment, and the starting material for skin cells to "produce" melanin is tyrosine. Tyrosine is a very common amino acid in the human body. In melanocyte melanocytes, tyrosine is catalyzed by tyrosinase to generate dopa, and dopa is dehydrogenated to form dopaquinone, which is rearranged into 5 , 6 hydroxyindole, after indole polymerizes, it combines with structural proteins in melanosomes to form melanin. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61Q19/02A23B7/154
CPCA23B7/154A61K2800/782A61Q19/02A61K8/9789
Inventor 杨子明李典鹏蔡爱华杨甲月刘金磊
Owner GUANGZHOU XIAOJING BIOTECH CO LTD
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