Method for preparing cardamine selenium polypeptide through continuous enzymolysis and cardamine selenium polypeptide

A technology of broken rice and enzymatic hydrolysis is applied in the field of continuous enzymatic hydrolysis to prepare broken rice selenium polypeptide and broken rice selenium polypeptide, which can solve the problems of non-reusable enzymes, high production cost, low production efficiency, etc. stability and reusability, improve stability, and achieve the effect of efficient utilization

Pending Publication Date: 2021-03-09
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Intermittent protein hydrolysis has many disadvantages: for example, the enzyme cannot be reused, the production cost is high, the product quality of each batch is difficult to control, the reaction time is long, and the production efficiency is low.

Method used

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  • Method for preparing cardamine selenium polypeptide through continuous enzymolysis and cardamine selenium polypeptide
  • Method for preparing cardamine selenium polypeptide through continuous enzymolysis and cardamine selenium polypeptide
  • Method for preparing cardamine selenium polypeptide through continuous enzymolysis and cardamine selenium polypeptide

Examples

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Effect test

Embodiment 1

[0059] This example provides a method for continuous enzymatic hydrolysis to prepare selenopolypeptides from the broken rice chestnut, see figure 1 Process flow chart, the steps are as follows:

[0060] (1) Preparation of broken rice chestnut protein: use a high-speed multifunctional grinder to crush the dried rice chestnut, pass through an 80-mesh sieve, and use deionized water and 0.1mol / L NaOH solution successively according to the mass ratio of material to liquid: 1g: 40mL Extracted separately for 8 hours, combined the extracts to obtain the protein liquid of broken rice chestnut, added ammonium sulfate to the protein liquid of broken rice chestnut to a saturation of 60%, redissolved the precipitate after centrifugation with deionized water, and carried out in a 3500Da dialysis bag After dialysis, the dialysate was freeze-dried to obtain the broken orina protein powder.

[0061] (2) Construction of immobilized enzyme: Dissolve tannic acid in 10mM Tris-HCl buffer solution ...

Embodiment 2

[0066] The difference with embodiment 1 is:

[0067] In step (2), place the above-mentioned substrate with a fixed coating in an alkaline protease solution of 1 g / L at room temperature and stir at 150 rpm / min for 12 h, then rinse with deionized water;

[0068] In step (3), the pH value is adjusted to 9 with NaOH solution.

Embodiment 3

[0070] The difference with embodiment 1 is:

[0071] In step (2), the above-mentioned matrix with fixed coating is placed in the alkaline protease solution of 1g / L (pH=9.010Mm boric acid buffer solution)

[0072] In step (3), the temperature is heated to 40°C.

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Abstract

The invention belongs to the field of peptide preparation, and particularly relates to a method for preparing cardamine selenium polypeptide through continuous enzymolysis and the cardamine selenium polypeptide. The method comprises the following steps of: preparing cardamine protein powder; constructing immobilized enzyme; performing pre-enzymolysis; performing ultrafiltration and material supplementation; concentrating and drying. The method integrates reaction, product separation and enzyme recycling, and can realize continuous operation. According to the method, substrate protein is subjected to continuous enzymolysis through an immobilized enzyme membrane reactor, so that a reaction product can be selectively removed, the product inhibition phenomenon is reduced, and the reaction rateand the substrate conversion rate are increased; through the selectivity of a membrane, the molecular weight of a hydrolysate can be controlled; the molecular weight of the cardamine selenium polypeptide obtained by the method is intensively distributed at 300-3,000Da, and the DH (degree of hydrolysis) can reach about 27 percent and is increased by 8.4 percent compared with the DH value of the conventional intermittent enzymolysis.

Description

technical field [0001] The invention belongs to the field of peptide preparation, and more specifically relates to a method for continuously enzymatically hydrolyzing and preparing the selenium polypeptide from the broken rice chestnut and the broken rice chestnut selenium polypeptide. Background technique [0002] Studies have shown that enzymatic reactions are the most common strategy for obtaining bioactive peptides from proteins. Compared with protein, hydrolyzed peptide is easier to absorb, has low sensitivity, high bioavailability, and fewer side effects. In recent years, with the in-depth research on selenium nutrition and bioactive peptides, it has been found that selenium peptides are one of the important sources of organic selenium in the diet, and natural selenium-rich peptides can also be obtained by enzymatically hydrolyzing selenium-rich proteins in raw materials. At present, there are many kinds of raw materials for preparing natural selenium-enriched peptide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/34C07K1/30C12N11/14
CPCC12P21/06C07K1/145C07K1/34C07K1/303C12N11/14C12N9/50
Inventor 祝振洲孙峥李书艺丛欣程水源
Owner WUHAN POLYTECHNIC UNIVERSITY
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