Method for preparing peptide of decrease blood pressure in laver by using enzyme-membrane coupling technique and application thereof

A technology of blood pressure-lowering peptide and seaweed, which is applied in the field of development and utilization of marine plant protein, can solve problems such as large consumption of enzymes, inability to apply industrialized production, and complexity, so as to improve product quality, reduce the operation of inactivating enzymes, and realize recycling. Effect

Inactive Publication Date: 2005-10-26
JIANGSU UNIV
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  • Claims
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AI Technical Summary

Problems solved by technology

[0006] At present, there are a small number of studies on the preparation of ACE-inhibiting peptides (referred to as "lowering blood pressure peptides") by enzymatic hydrolysis of food proteins, but traditional processes often have problems such as continuou

Method used

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  • Method for preparing peptide of decrease blood pressure in laver by using enzyme-membrane coupling technique and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Weigh 10kg of dried laver, crush it, and under the conditions of 30MPa and 35°C, use supercritical CO 2 Extraction technology removes the oil therein, and the EPA+DHA in the obtained laver oil reaches 50%, which can be used to develop auxiliary blood fat-lowering functional food. Then, add 10 times the weight of deionized water to the degreased laver powder, stir and dissolve, so that the solution concentration is 10%, and circulate the ultrasonic action in the ultrasonic treatment equipment for 20 minutes.

[0022] Measure 10L of ultrasonically treated laver solution, add alkaline protease according to the ratio of 25AU protease / kg solution, and carry out enzymatic hydrolysis under the conditions of pH 9 and temperature 60°C. The enzymatic hydrolysis reaction solution is first filtered with a cloth bag, and then sent to the ultrafiltration equipment. The molecular weight cut-off of the ultrafiltration membrane is 5kDa, and the ultrafiltration pressure is 0.5mPa. The ...

Embodiment 2

[0025] Weigh 5 kg of degreased laver powder, add 20 times the weight of deionized water, stir and dissolve, so that the solution concentration is 5%, and circulate the ultrasonic action in the ultrasonic treatment equipment for 35 minutes.

[0026] Measure 10L of ultrasonically treated seaweed solution, add neutral protease at a concentration of 15AU protease / kg, and perform enzymatic hydrolysis at pH 6.8 and temperature 45°C. The enzymatic hydrolysis reaction solution is first filtered with a cloth bag, and then sent to the ultrafiltration equipment. The molecular weight cut-off of the ultrafiltration membrane is 10kDa, and the ultrafiltration pressure is 0.3mPa. The ultrafiltration membrane retentate is returned to the enzymatic hydrolysis reactor. The permeate of the ultrafiltration membrane is the obtained laver hypotensive peptide solution. According to the content of polypeptide and moisture in the permeate, set the ratio of replenishing laver solution to the enzymolys...

Embodiment 3

[0029] Weigh 5 kg of degreased laver powder, add 15 times the weight of deionized water, stir and dissolve, so that the solution concentration is 6.67%, and circulate the ultrasonic action in the ultrasonic treatment equipment for 25 minutes.

[0030] Measure 10L of ultrasonically treated laver solution, add compound protease at a concentration of 21AU / kg, and perform enzymatic hydrolysis under the conditions of pH 5.2 and temperature 30°C. The enzymatic hydrolysis reaction solution is first filtered with a cloth bag, and then sent to the ultrafiltration equipment. The molecular weight cut-off of the ultrafiltration membrane is 10kDa, and the ultrafiltration pressure is 0.5mPa. The ultrafiltration membrane retentate is returned to the enzymatic hydrolysis reactor. The permeate of the ultrafiltration membrane is the obtained laver hypotensive peptide solution. According to the content of polypeptide and moisture in the permeate, set the ratio of replenishing laver solution to...

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Abstract

This invention provided a method of using enzyme-membrane coupling technology to prepare laver peptide of lowering blood pressure. It consists of the way: after the process of smashing, the degreasing, the dissolution, supersonic affects, laver solution must be extracted out peptide of lowering blood pressure by the above technology, at last obtain the product by filter, condense, dry course. This invention adapt the above technology, at the period of enzyme decompose process, the supersonic affects was used to filter the production out of the system of separation. It carries out quick reaction of enzyme decompose and circle usage of proteinase, enhances the quality of production, shortens the time of disinfect operation.

Description

Technical field: [0001] The invention relates to the field of development and utilization of marine plant protein, in particular to health food production and biopharmaceutical technology, in particular to a method for preparing laver hypotensive peptide using enzyme-membrane coupling technology and its application. Background technique: [0002] Seaweed is rich in proteins, polypeptides, amino acids, nucleic acids, sugars, fatty acids, vitamins, inorganic elements, etc., and its nutritional value is very rich. [0003] There have been some publications on the development and utilization of laver both at home and abroad. For example, the patents published and authorized in my country include: "Using Porphyra laver to produce high-absorbance phycoerythrin and its method" (publication number 1520718), using Porphyra long-leaf as raw material to extract phycoerythrin with high absorbance ratio. By increasing the ratio of phycoerythrin per unit weight, the effect of reducing th...

Claims

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Application Information

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IPC IPC(8): C12P21/02
CPCY02P20/582
Inventor 马海乐骆琳何荣海
Owner JIANGSU UNIV
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