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Method for improving yield of soybean peptide with low molecular weight

A low-molecular-weight, soybean peptide technology, applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of large molecular weight of soybean peptide products, insufficient clarity and transparency of the solution, dark solution color, etc. The effect of high peptide content and low molecular weight

Active Publication Date: 2014-04-02
NUOLI RUYI ANYANG BIOLOGICAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the existing soybean peptide production and processing enterprises usually adopt the following production and processing methods: soybean protein isolate is dissolved, enzymatically hydrolyzed, centrifuged, sterilized, flash deodorized, high-temperature concentrated, spray-dried, and packaged; The soybean peptide product has a large molecular weight and uneven distribution. It will form a precipitate in an acidic solution, and the solution is very dark after dilution, and the solution is not clear enough.

Method used

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  • Method for improving yield of soybean peptide with low molecular weight

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Effect test

Embodiment 1

[0021] A kind of method that the present embodiment provides to improve the yield of low molecular weight soybean peptide comprises the following steps:

[0022] (1) Weigh soybean protein isolate, add water, and after the soybean protein isolate is fully dissolved, adjust the pH of the soybean protein isolate solution to 6;

[0023] (2) After sterilizing the soybean protein isolate solution obtained in step (1), add a compound protease for enzymolysis to obtain an enzymolysis solution. The sugar content of the liquid is 5BX, and the pH value is 6;

[0024] (3) After treating the enzymolysis solution obtained in step (2) with a microfiltration membrane, add water to wash the filtrate, and collect the filtered soybean peptide mixture; the sugar content of the obtained filtrate is 4BX, and the water in the cleaning process The weight ratio with the filtrate is 1:1;

[0025] (4) The soybean peptide mixture obtained in step (3) is subjected to nanofiltration operation, and the co...

Embodiment 2

[0028] A kind of method that the present embodiment provides to improve the yield of low molecular weight soybean peptide comprises the following steps:

[0029] (1) Weigh soybean protein isolate, add water, and after the soybean protein isolate is fully dissolved, adjust the pH of the soybean protein isolate solution to 8;

[0030] (2) After sterilizing the soybean protein isolate solution obtained in step (1), add a compound protease for enzymolysis to obtain an enzymolysis solution. The sugar content of the liquid is 10BX, and the pH value is 8;

[0031] (3) After treating the enzymolysis solution obtained in step (2) with a microfiltration membrane, add water to wash the filtrate, and collect the filtered soybean peptide mixture; the sugar content of the obtained filtrate is 10BX, and the water in the cleaning process The weight ratio with the filtrate is 1:5;

[0032] (4) The soybean peptide mixture obtained in step (3) is subjected to nanofiltration operation, and the ...

Embodiment 3

[0035] A kind of method that the present embodiment provides to improve the yield of low molecular weight soybean peptide comprises the following steps:

[0036] (1) Weigh soybean protein isolate, add water, and after the soybean protein isolate is fully dissolved, adjust the pH of the soybean protein isolate solution to 8;

[0037] (2) After sterilizing the soybean protein isolate solution obtained in step (1), add a compound protease for enzymolysis to obtain an enzymolysis solution. The sugar content of the liquid is 18BX, and the pH value is 8;

[0038] (3) After treating the enzymolysis solution obtained in step (2) with a microfiltration membrane, add water to wash the filtrate, and collect the filtered soybean peptide mixture; the sugar content of the obtained filtrate is 15BX, and the water in the cleaning process The weight ratio with the filtrate is 1:15;

[0039] (4) Perform nanofiltration operation on the soybean peptide mixture obtained in step (3), and collect ...

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Abstract

The invention discloses a method for improving yield of soybean peptide with low molecular weight, which employs an enzyme-membrane method for carrying out deep processing of isolated soybean protein, and controls yield of reaction by controlling sugar degree of enzymatic hydrolysis liquid, and controls product yield by controlling sugar degree of filtrate and water addition, and controls product transparency by controlling concentrate sugar degree. The invention has the advantages of high peptide content, clear and transparent solution, high yield rate, low soybean peptide molecular weight and easy absorption, and even in acidic solution, the soy peptides do not precipitate, and can be widely applied in the fields such as foodstuff, cosmetic, etc.

Description

technical field [0001] The invention relates to the technical field of soybean protein deep processing, in particular to a method for increasing the yield of low-molecular-weight soybean peptides. Background technique [0002] With the improvement of living standards, people pay more and more attention to dietary health. As for the way of protein intake, people are no longer limited to only taking protein from animals, but more from the perspective of health to take some cholesterol-free vegetable protein. Compared with other vegetable proteins, soybean protein has the most comprehensive nutrition. value. However, because soybean protein has many limitations in processing, in practical applications, soybean protein is usually processed into soybean peptides that can be more easily utilized by people, and at the same time, the original functions of soybean protein will not be affected. [0003] At present, the existing soybean peptide production and processing enterprises u...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 岳炳祥付慧彦张海萍张新伟
Owner NUOLI RUYI ANYANG BIOLOGICAL SCI & TECH
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