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Boron-doping diamond film based ascorbic acid oxidase sensor electrode

A boron-doped diamond, sensor electrode technology, applied in the field of ascorbate oxidase sensor electrodes, can solve the problems of inability to carry out electrochemical oxidation process, low response speed and reproducibility, low measurement sensitivity, etc., and achieve excellent physical and chemical properties, high Detection limit and reproducibility, the effect of high detection sensitivity

Inactive Publication Date: 2012-07-18
TIANJIN UNIVERSITY OF TECHNOLOGY
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Problems solved by technology

At present, the determination of ascorbic acid includes iodometric method, voltammetric method, biosensor, HPLC, capillary electrophoresis and flow injection analysis, etc., most of which involve complex operations and expensive instruments
When some determination methods are used to determine the content of ascorbic acid in serum, due to poor anti-pollution ability, low detection sensitivity, low response speed and low reproducibility
[0003] When detecting ascorbic acid in human serum, due to the passivation pollution of the electrode by the oxidation product of ascorbic acid, the electrode is "poisoned", the current is reduced, and the electrochemical oxidation process cannot be carried out.
At the same time, due to its high overpotential on general electrodes, it has low sensitivity and poor reproducibility.

Method used

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  • Boron-doping diamond film based ascorbic acid oxidase sensor electrode
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  • Boron-doping diamond film based ascorbic acid oxidase sensor electrode

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Embodiment

[0029] A preparation method of the ascorbate oxidase sensor electrode based on the boron-doped diamond film, the steps are as follows:

[0030] 1) Pre-treat the surface of the tantalum substrate: first, mechanically polish the tantalum substrate to remove the oxide layer and pollutants on the surface, then perform mechanical grinding of corundum to cause scratches and defects on the surface of the tantalum substrate, and finally conduct surface ultrasonic deal with;

[0031] 2) Deposit a boron-doped diamond film on the surface of the pretreated tantalum sheet substrate using hot wire CVD equipment: put the pretreated substrate into the reaction chamber of the hot wire CVD equipment to deposit the diamond film, and the hot wire CVD equipment uses tantalum The filament is used as the heat source, the filament temperature is 2200°C, the deposition process parameters: the reaction gas source is a mixture of hydrogen and acetone, the flow ratio of hydrogen and acetone in the mixtur...

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Abstract

The invention discloses a boron-doping diamond film based ascorbic acid oxidase sensor electrode which is composed of a boron-doping diamond film, an amino monomolecular layer and an enzyme membrane layer. The preparation method of the ascorbic acid oxidase sensor electrode comprises the steps of: firstly, depositing a boron-doping diamond film on a tantalum sheet which is subjected to a surface pre-treatment by utilizing a hot filament CVD (Chemical Vapor Deposition) device; then performing amination modification on the boron-doping diamond film; and finally fixing the ascorbic acid oxidase on the surface of the ammoniated diamond film by adopting a chemical crosslinking way, wherein the electrode can be used on an electrochemical biosensor. The boron-doping diamond film based ascorbic acid oxidase sensor electrode, disclosed by the invention, has the advantages of having the limit of detection of 1*10-11 mol / L, and having high sensitivity, good reproducibility and long service life as the electrode inherits the advantages of corrosion resistance, extremely high oxygen evolution overvoltage, wide electric potential window, high electrochemical stability and the like of the diamond.

Description

technical field [0001] The invention belongs to the field of electrochemical biosensors, in particular to an ascorbic acid oxidase sensor electrode based on a boron-doped diamond film. Background technique [0002] Ascorbic acid (vitamin C) participates in a variety of metabolic processes in the human body and is one of the important vitamins for maintaining human health. The lack of it will lead to scurvy and the decline of the body's disease resistance. Therefore, the determination of its content in serum and urine is an important disease diagnosis and Nutritional evaluation indicators. At present, the determination of ascorbic acid includes iodometric method, voltammetric method, biosensor, HPLC, capillary electrophoresis and flow injection analysis, etc., most of which involve complex operations and expensive instruments. When some determination methods are used to determine the content of ascorbic acid in serum, due to poor anti-pollution ability, low detection sensiti...

Claims

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Application Information

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IPC IPC(8): G01N27/327
Inventor 朱宁申风婷姜啸宇曲长庆张辉戴玮张聪聪尹振超
Owner TIANJIN UNIVERSITY OF TECHNOLOGY
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