Process of continuous production of casein bioactive peptide by enzymolysis and filtering membrane concentration

A technology for biologically active polypeptides and casein, which is applied in the field of enzymatic hydrolysis to prepare active polypeptides, which can solve the problems that the enzymatic hydrolysis products cannot be separated from the reaction system in time, the conversion rate of the enzymatic hydrolysis reaction is low, the yield of the target polypeptide is low, and the product quality is unstable and other problems, to achieve the effect of prolonging the effective reaction time, tending to uniform composition and stable quality

Inactive Publication Date: 2004-11-17
TIANJIN UNIV
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Problems solved by technology

[0004] At present, intermittent enzymatic hydrolysis is widely used in the production process of casein bioactive polypeptides, that is, batch operations are carried out in ordinary hydrolysis tanks. Casein is precipitated and centrifuged, followed by multi-step separation and purification steps such as solvent selective precipitation or ion exchange resin treatment to obtain the target polypeptide product. The following problems exist in the process: (1) The enzymatic hydrolysis product cannot be obtained from the reaction Timely separation in...

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  • Process of continuous production of casein bioactive peptide by enzymolysis and filtering membrane concentration

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Embodiment 1

[0014] Weigh 800g of casein, add 20L of water and place it in a liquid storage tank, heat slightly and stir well, slowly add 16mL of 1g / mL NaOH solution to prepare a casein substrate solution with a pH of 8.5 and a concentration of 4%. Put it into the hydrolysis tank, adjust the stirring speed to 80rmp, start the constant temperature water bath, and after the temperature is constant at 45°C, open the ultrafiltration circulation device, the average molecular weight cut-off of the ultrafiltration membrane is 10000, and the substrate solution is circulated at a flow rate of 30L / h. Stable circulation at normal pressure for 15 minutes, add 20 grams of alkaline protease to the hydrolysis tank, start the continuous enzymatic hydrolysis reaction and record the time, after 10 minutes, adjust the operating pressure of the ultrafiltration membrane to 0.05MPa, casein, protease and macromolecular polypeptides and other macromolecular substances It is intercepted by the ultrafiltration membr...

Embodiment 2

[0016] Adopting the ultrafiltration membrane that the average molecular weight cut off is 6000 and the nanofiltration membrane that the average molecular weight cut off is 2000 and 20L enzymolysis tank form continuous reaction separation system, add 20g trypsin to 20L mass concentration and be 2% casein substrate solution, During the reaction process, keep the system temperature at 40°C and pH=8.0 constant, continuously add 1% casein substrate solution to keep the reaction volume and effective substrate concentration constant, add 4g of trypsin every 4 hours to maintain the constant enzyme activity of the system, Other enzymatic hydrolysis reactions and membrane separation operation steps and process parameters are the same as in Example 1, and the active polypeptide products mainly composed of casein phosphopeptides are obtained. The products are detected and analyzed by HPSEC, and the molecular weights are mainly concentrated in the corresponding peaks of 3000-5000. Nitrogen ...

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Abstract

The invention relates to a process of continuous production of casein bioactive peptide by enzymolysis and filtering membrane concentration which comprises, using caseinum as raw material and at least one prolease action, obtaining biologically active polypeptides having multiple functions in multi-stage enzyme membrane reactor combined from enzymolysis tank and hyperfiltration, nano filter membrane of dissimilar entrapment molecular weight.

Description

technical field [0001] The invention relates to a new process for continuously producing casein-derived polypeptides by integrating enzymatic hydrolysis reaction and membrane separation technology, and belongs to the technology for producing active polypeptides by enzymatically hydrolyzing casein as a substrate. Background technique [0002] Casein is the protein with the highest content in milk. It is a complex of several phosphorus-containing proteins, and its main component is α s1 、α s2 , β, κ four polypeptide chains. Casein is hydrolyzed by a single enzyme or a complex enzyme system, and then separated and purified to obtain nearly 10 categories of more than 100 biologically active polypeptides. Complex enzymatic hydrolysis products and diverse sequence structures make this kind of substances have various physiological functions such as immune regulation, free radical scavenging, mineral carrier, anticancer and antibacterial, thrombolysis and blood pressure reduction,...

Claims

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Application Information

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IPC IPC(8): C12P21/00
Inventor 何志敏齐崴何明霞乔文邹超
Owner TIANJIN UNIV
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