Method for preparing xylonic acid (salt) through whole-cell high-efficiency catalysis of xylose transformation

A high-efficiency catalysis of xylose and xylonic acid, which is applied in the field of efficient whole-cell catalysis of xylose conversion to produce xylonic acid (salt), to achieve the effect of increasing the reaction concentration

Inactive Publication Date: 2012-09-19
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the existing chemical synthesis and enzymatic conversion of xylose to produce xylonic acid (salt) do not have the conditions for industrial production, and the microbial fermentation method still needs to further improve the su

Method used

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  • Method for preparing xylonic acid (salt) through whole-cell high-efficiency catalysis of xylose transformation
  • Method for preparing xylonic acid (salt) through whole-cell high-efficiency catalysis of xylose transformation

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0019] Example 1

[0020] In a 3.0L fully automatic mechanically stirred and ventilated fermenter, add 1500mL xylose solution with an initial xylose mass concentration of 9.5%, add 0.4 g / L magnesium sulfate, 1.5 g / L dipotassium hydrogen phosphate and 1.0 g / L sulfuric acid Ammonium, control the stirring rate to 300r / min, pass in sterile compressed air to a dissolved oxygen concentration of 10%~50%, and connect the activated Gluconobacter oxydans NL71 to a cell concentration of OD 600nm = 1.0, add powdered calcium carbonate to adjust the pH of the reaction system to about 6.5, and perform a whole-cell catalytic reaction.

[0021] Monitor the pH value and xylose concentration of the reaction system in real time, and add about 100g of powdered solid xylose and calcium carbonate in 3 batches every 20~24h, and control the pH to 3.0~6.5 and the mass concentration of xylose not higher than 18% (G / mL).

[0022] Such as figure 1 , After reaction to 192h, the mass concentration of xylose in...

Example Embodiment

[0023] Example 2

[0024] In a 10.0L fully automatic mechanically ventilated and stirred fermentation tank, add 4L xylose solution with an initial xylose mass concentration of 15%, add 0.4 g / L magnesium sulfate, 1.5 g / L dipotassium hydrogen phosphate and 1.0 g / L sulfuric acid Ammonium, control the stirring rate to 280r / min, pass in sterile compressed air to a dissolved oxygen concentration of 10%~50%, and connect to the activated ATCC 621-H strain to a cell concentration of OD 600nm = 1.1, add powdered calcium carbonate to adjust the pH of the reaction system to about 6.5, and perform a whole-cell catalytic reaction.

[0025] Real-time monitoring of the pH value and xylose concentration of the reaction system, continuous addition of solid xylose, and real-time control of the pH value of 3.0 to 6.5 and the mass concentration of xylose not higher than 25%.

[0026] The cumulative added mass concentration of xylose added to the reaction system reached 30%, and the addition was stopped,...

Example Embodiment

[0027] Example 3

[0028] In a 100 L fully automatic mechanically ventilated and stirred fermentation tank, add 70L of xylose solution with an initial xylose mass concentration of 14%, add 0.4 g / L magnesium sulfate, 1.5 g / L dipotassium hydrogen phosphate and 1.0 g / L sulfuric acid Ammonium, control the stirring rate to 220r / min, pass in sterile compressed air to a dissolved oxygen concentration of 15% to 40%, and connect to the activated ATCC 621-H strain to a cell concentration of OD 600nm = 1.1, add powdered calcium carbonate to adjust the pH of the reaction system to about 6.5, and perform a whole-cell catalytic reaction.

[0029] Real-time monitoring of the pH value and xylose concentration of the reaction system, adding high-concentration xylose solution in batches, and real-time control of the pH value of 3.0 to 6.5 and the mass concentration of xylose not higher than 20%.

[0030] The cumulative added mass concentration of xylose added to the reaction system reached 29.2%, the...

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Abstract

The invention discloses a method for preparing xylonic acid (salt) through whole-cell high-efficiency catalysis of xylose transformation. The method comprises the following steps of: adding an initial reaction matrix into a liquid deep-layer ventilation reaction system; inoculating gluconabacteriumoxydonas; stirring and ventilating to continuously react; keeping dissolved oxygen concentration of the reaction system not less than 10 percent; adding xylose or xylose solution into the reaction system in a semi-continuous or continuous batch adding mode; controlling mass concentration of xylose in the reaction system to be not greater than 25 percent; synchronously adjusting and controlling the pH value to be not less than 2.8; stopping adding when the mass concentration of the xylose accumulatively added into the reaction system reaches 30 percent; and continuously performing full catalytic reaction to obtain a transformed product, i.e., the xylose acid (salt) product. According to the method, the maximum value of the accumulative concentration of the substrate xylose can reach 30 percent, the maximum utilization rate of the xylose exceeds 99 percent, the maximum concentration value of the fermentation product, i.e., the xylose acid (salt), can reach 29.8 percent, and the transformation ratio of the xylose acid (salt) can reach 100 percent.

Description

technical field [0001] The invention relates to a preparation method of xylonic acid (salt), in particular to a method for preparing xylonic acid (salt) by efficiently catalyzing xylose conversion in whole cells. Background technique [0002] The efficient conversion and utilization of xylose is a key technical bottleneck in the processing and utilization of lignocellulosic raw materials, especially in its biorefinery. At present, the main products of xylose bioconversion are fuel ethanol, xylitol, xylose acid (salt), lactic acid, succinic acid and feed yeast. Except for xylonic acid (salt), due to the limitation of market capacity, environmental pollution and existing production technology level, the development space of other products is very limited at present. [0003] Xylonic acid (salt) has similar properties to gluconic acid (salt), and can be used as a substitute for products such as citric acid and gluconic acid. As an emerging bio-based chemical, its use has been ...

Claims

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Application Information

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IPC IPC(8): C12P19/02C12R1/01
Inventor 徐勇勇强余世袁王荥
Owner NANJING FORESTRY UNIV
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