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Mediator electrochemical enzyme electrode and method for making same

An electrochemical and enzyme electrode technology, applied in the field of mediator electrochemical enzyme electrode and its preparation, can solve problems such as enzyme shedding and low sensor stability, achieve small diffusion resistance, easy product separation process, and widen linear response range Effect

Inactive Publication Date: 2008-10-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the excellent hydrophilicity of chitosan and the shedding of enzymes, there may still be a problem of low sensor stability during electrochemical analysis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) earlier acrylonitrile copolymer (viscosity-average molecular weight 100,000, comonomer mole percent is 5%) and aminoferrocene are dissolved in dimethylformamide, and the mass ratio of acrylonitrile copolymer and electron mediator is 1 / 1 Then add N,N'-dicyclohexylcarbodiimide, its concentration in dimethylformamide is 5 mg / ml, react at 15°C for 12 hours. The product was precipitated with absolute ethanol, washed, and vacuum-dried at 60° C. for 24 hours to obtain a ferrocene-functionalized acrylic acid-containing acrylonitrile copolymer;

[0031] 2) dissolving the acrylic acid-containing acrylic acid-containing acrylonitrile copolymer functionalized by ferrocene in dimethylformamide, and preparing a copolymer solution with a mass fraction of 3%; using a platinum electrode as a receiving plate, when the voltage is 10 kilovolts 1. When the extrusion rate is 0.5 ml / hour and the receiving distance is 10 cm, the fiber film is collected, and the spinning time is continued fo...

Embodiment 2

[0034] 1) earlier acrylonitrile copolymer (viscosity-average molecular weight 30,000, comonomer mole percent is 20%) and amino-containing porphyrin are dissolved in dimethylformamide, the mass ratio of acrylonitrile copolymer and electronic medium 5 / 1, then add N, N'-dicyclohexylcarbodiimide, its concentration in dimethylformamide is 15 mg / ml, and react at 45°C for 48 hours. The product was precipitated with absolute ethanol, washed, and vacuum-dried at 70° C. for 48 hours to obtain a porphyrin-functionalized acrylonitrile copolymer containing methacrylic acid;

[0035] 2) dissolving the acrylonitrile copolymer containing methacrylic acid functionalized by porphyrin in dimethylformamide, and preparing a copolymer solution with a mass fraction of 3% to 10%; using a gold electrode as a receiving plate, when the voltage 20 kV, an extrusion rate of 3.0 ml / hour, and a receiving distance of 20 cm to collect the fiber film, and the spinning time continued for 10 hours to obtain an el...

Embodiment 3

[0038] 1) earlier acrylonitrile copolymer (viscosity-average molecular weight 30,000, comonomer mole percent is 20%) and amino-containing iron porphyrin are dissolved in dimethylformamide, the quality of acrylonitrile copolymer and electronic medium The ratio is 1 / 1, then N,N'-dicyclohexylcarbodiimide is added, its concentration in dimethylformamide is 15 mg / ml, and reacted at 45°C for 48 hours. The product was precipitated with absolute ethanol, washed, and vacuum-dried at 70°C for 48 hours to obtain an iron porphyrin-functionalized acrylonitrile copolymer containing methacrylic acid;

[0039] 2) dissolving the acrylonitrile copolymer containing methacrylic acid functionalized by iron porphyrin in dimethylformamide, and preparing a copolymer solution with a mass fraction of 5%; taking the glassy carbon electrode as the receiving plate, when the voltage is 20 kV, the extrusion rate is 3.0 ml / hour, and the receiving distance is 20 cm to collect the fiber film, and the spinning ...

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Abstract

The invention discloses a mediator electrochemical enzyme electrode and a method for making the same. Acrylonitrile copolymer with a functionalized mediator is prepared through the coupling reaction of acrylonitrile copolymer and an electric mediator with a reactive group in dimethylformamide. The dimethylformamide is taken as solvent, the functionalized acrylonitrile copolymer is taken as a raw material, an electrochemical electrode is taken as an accepting electrode, a copolymer fiber membrane is prepared on the surface of the electrode through an electrostatic spinning method, then a covalent method is used to fix xidoreductase on a fiber layer on the surface of the electrode to form an activated layer of fibers. In the invention, extremely high specific surface area of the electrostatic spinning fiber, the reaction group on the surface of the electrostatic spinning fiber and the fixed electric mediator ensure that the electrochemical enzyme electrode has higher enzyme-carrying efficiency, high stability and high response strength. The mediator electrochemical enzyme electrode has the advantages of simple preparation process, repeated use, large surface area of an effective enzyme membrane, strong anti-jamming ability and so on, and is suitable for batch production.

Description

technical field [0001] The invention relates to a mediator electrochemical enzyme electrode and a preparation method thereof. Background technique [0002] So far, the development of biosensors has gone through nearly fifty years, attracting great attention from scientists all over the world, and is being applied to more fields. The composition of biosensors includes two parts: molecular recognition elements and transducers. The measured substance diffuses into the biologically sensitive membrane layer, and after molecular recognition and biochemical reaction, the information generated is converted into an electrical signal related to the concentration of the measured substance by the corresponding transducer. Molecular recognition elements play a key role in determining the function of biosensors, which contain biological components such as enzymes, tissues, antibodies / antigens, bacteria, and yeasts. Immobilized enzyme electrodes have received the most research and applic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 徐志康王振刚黄小军
Owner ZHEJIANG UNIV
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