Preparation method of fixed enzyme membrane

A technique of immobilizing enzymes and polypropylene membranes, which is applied in the direction of fixing on/in organic carriers, can solve the problems of low catalytic activity and low enzyme protein loading capacity of immobilized enzyme membranes, and achieve good catalytic activity, Increased protease loading and the effect of increasing the enzyme protein loading capacity

Inactive Publication Date: 2010-07-21
HARBIN UNIV OF COMMERCE
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Problems solved by technology

[0004] The present invention provides a method for preparing an immobilized enzyme membrane in order to solve the problems of l

Method used

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  • Preparation method of fixed enzyme membrane

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specific Embodiment approach 1

[0010] Specific embodiment 1: The preparation method of the immobilized enzyme membrane of this embodiment is carried out according to the following steps: 1. The polypropylene membrane is soaked in acetone for 22-26 hours, then dried at 25-35° C. for 22-26 hours, and then put into a concentration of In the acetone solution of 0.2mol / L benzophenone, under the protection of nitrogen, irradiate with ultraviolet light for 14-16min, and the intensity of ultraviolet light irradiation is 90-110μW / cm 2 , the irradiation distance is 11-13cm, and then the film is dried naturally and immersed in an ethanol solution of methyl methacrylate with a mass concentration of 10%-30%, shaken in a water bath at 28-32°C for 22-26h, and then at 28 Vacuum drying under the condition of ~32 ° C for 22 ~ 26h can obtain the methyl methacrylate grafted film, wherein the solvent of the ethanol solution of methyl methacrylate is ethanol with a mass concentration of 90% ~ 99%; two, methacrylic acid The methy...

specific Embodiment approach 2

[0014] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 1, the polypropylene film is soaked in acetone for 24 hours. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0015] Embodiment 3: This embodiment is different from Embodiments 1 to 2 in that: in step 1, dry at 30° C. for 24 hours. Other steps and parameters are the same as those in Embodiments 1 to 2.

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Abstract

The invention relates to a preparation method of a fixed enzyme membrane. The invention solves the problems of low enzyme protein carrying capacity and low catalytic activity of a fixed enzyme membrane prepared by the traditional method. The method comprises the following steps of: (1) preparing a methyl methacrylate grafted membrane; (2) preparing a methyl methacrylate grafted membrane with absorbed hexanediamine; and (3) soaking and processing the methyl methacrylate grafted membrane with the absorbed hexanediamine into a bacillus licheniformis alkaline protease solution or a transglutaminase solution so as to obtain a fixed enzyme membrane. The fixed enzyme membrane prepared by the invention has good enzyme protein carrying capacity, bacillus licheniformis alkaline protease carrying capacity larger than 35mg/g membrane and transglutaminase carrying capacity larger than 20mg/g membrane. The fixed enzyme membrane prepared by the method has good absorption capability, catalytic activity and thermal stability.

Description

technical field [0001] The invention relates to a preparation method of immobilized enzyme. Background technique [0002] Immobilized enzyme is a new technology that binds or confines the active enzyme to a certain area with certain materials, but can still carry out the catalytic reaction unique to the enzyme, and can be recycled and reused. Compared with water-soluble enzymes, immobilized enzymes not only maintain their high efficiency, specificity, mildness, and adjustable enzyme activity, but also have the advantages of easy separation and recovery, repeated use, continuous and controllable operation, Due to the series of advantages such as simple process, it has developed rapidly in related fields such as biology and bioengineering, medicine, food, medicine and life sciences. [0003] Since membrane separation technology entered industrialization in the 1950s, after decades of development, it has widely penetrated into the fields of chemical industry, metallurgy, envir...

Claims

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Application Information

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IPC IPC(8): C12N11/08
Inventor 马永强石彦国杨春华张毅方刘颖
Owner HARBIN UNIV OF COMMERCE
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