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Preparation method of corn oligopeptide

A technology of corn oligopeptide and corn protein, which is applied in the biological field, can solve the problems such as the molecular weight distribution data of corn peptide crude protein content, etc., and achieve the effects of molecular weight concentration, purity improvement, and dosage reduction

Inactive Publication Date: 2013-04-03
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention was established on a laboratory scale, and the patent does not state the crude protein content of the corn peptides of the vegetation and the molecular weight distribution data of the peptides

Method used

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  • Preparation method of corn oligopeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Pretreatment of corn gluten meal

[0023] ①Crush corn gluten powder, pass through a 30-mesh sieve, and input it into the extraction tube from the feed port, and at the same time, input ethanol with a pH of 7, a concentration of 70%, and a temperature of 30°C into the extraction tube from the liquid inlet of the extractor. The liquid ratio is 1:4.

[0024] ②Corn gluten powder and ethanol were subjected to countercurrent ultrasonic extraction in the extraction tube of a continuous countercurrent ultrasonic extraction machine for 30 minutes. The conditions of ultrasonic extraction were: temperature 30°C, ultrasonic frequency 40kHz.

[0025] ③ After the countercurrent ultrasonic extraction is completed, the decolorized corn gluten powder is discharged from the slag discharge port of the continuous countercurrent ultrasonic extractor, and the decolorized corn gluten powder is used for subsequent enzymatic hydrolysis; it is discharged from the liquid discharge port of the...

Embodiment 2

[0034] (1) Pretreatment of corn gluten meal

[0035] ①Crush the corn gluten powder, pass through a 40-mesh sieve, and input it into the extraction tube from the feed port. The liquid ratio is 1:6.

[0036] ②Corn gluten powder and ethanol were subjected to countercurrent ultrasonic extraction in the extraction tube of a continuous countercurrent ultrasonic extraction machine for 60 minutes. The conditions of ultrasonic extraction were: temperature 40°C, ultrasonic frequency 40kHz.

[0037]③ After the countercurrent ultrasonic extraction is completed, the decolorized corn gluten powder is discharged from the slag discharge port of the continuous countercurrent ultrasonic extractor, and the decolorized corn gluten powder is used for subsequent enzymatic hydrolysis; it is discharged from the liquid discharge port of the continuous countercurrent ultrasonic extractor for extraction liquid.

[0038] ④ The extract is filtered to obtain a filtrate, concentrated to recover ethanol, a...

Embodiment 3

[0045] (1) Pretreatment of corn gluten meal

[0046] ①Crush corn gluten powder, pass through a 60-mesh sieve, and input it into the extraction tube from the feed port, and at the same time, input ethanol with a pH of 8.5, a concentration of 85%, and a temperature of 50°C into the extraction tube from the liquid inlet of the extractor , The ratio of solid to liquid is 1:8.

[0047] ②Corn gluten powder and ethanol were subjected to countercurrent ultrasonic extraction in the extraction tube of a continuous countercurrent ultrasonic extraction machine for 90 minutes. The conditions of ultrasonic extraction were: temperature 50°C, ultrasonic frequency 40kHz.

[0048] ③ After the countercurrent ultrasonic extraction is completed, the decolorized corn gluten powder is discharged from the slag discharge port of the continuous countercurrent ultrasonic extractor, and the decolorized corn gluten powder is used for subsequent enzymatic hydrolysis; it is discharged from the liquid discha...

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Abstract

Belonging to the field of biotechnologies, the invention relates to a preparation method of a corn oligopeptide. The method comprises: taking corn protein powder as the raw material, conducting a pretreatment by a continuous countercurrent ultrasonic technology, extracting yellow pigment, carrying out enzymolysis on the well treated raw material by an enzyme membrane reactor, collecting peptide fragments with a proper molecular weight, and performing concentrating and belt vacuum drying, thus obtaining the corn oligopeptide prepared with a lot of physiologically active functions. While preparing the corn oligopeptide, the corn oligopeptide preparation method disclosed in the invention also makes use of the continuous countercurrent ultrasonic extraction technology to extract corn yellow pigment. The process is simple, at the same time, the solvent consumption is reduced to an amount accounting for 40%-50% of that of traditional extraction methods, and the yield is increased by 20%-40%. By using the enzyme membrane reactor to perform enzymolysis on the protein powder, the controllability is good, the prepared corn oligopeptide has a concentrated molecular weight and purity improved by 10%-20%, and has the physiological function activities of hangover alleviating, liver protection, immunoregulation and the like.

Description

technical field [0001] The invention relates to a preparation method of corn oligopeptide, which belongs to the field of biotechnology. Background technique [0002] Corn is one of the three major food crops in the world. The corn deep-processing industry has huge market potential. Every year, tens of millions of tons of corn are deep-processed to produce starch, starch sugar, alcohol and other products. Corn gluten meal is one of the important by-products of corn starch production. It is rich in corn protein, but it has been used as livestock and poultry feed for many years and has not been well developed, resulting in a great waste of resources. [0003] Corn gluten powder is also called corn gluten powder, mainly composed of corn gluten, the protein is as high as more than 60%, and contains a small amount of starch and fiber. Corn protein is mainly composed of zein, glutenin, globulin and albumin. At present, corn gluten powder is used for feed or natural discharge. The ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34
Inventor 刘宗利王乃强王彩梅王明珠李从从李方华
Owner BAOLINGBAO BIOLOGY
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