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Method for in-situ preparation of nano-enzyme membrane

An in-situ preparation, nano-enzyme technology, applied in biochemical equipment and methods, oxidoreductases, enzymes, etc., can solve problems such as difficulty in reusing and recycling, and achieve improved hydrophilic properties, reduced water contact angle, and increased water contact angle. The effect of load rate

Active Publication Date: 2018-02-23
深圳市新纳捷科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of MOFs immobilized enzymes in wastewater treatment still has the problem of being difficult to recycle and reuse.

Method used

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  • Method for in-situ preparation of nano-enzyme membrane

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Experimental program
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Effect test

preparation example Construction

[0024] The preparation method of nano enzyme film of the present invention may further comprise the steps:

[0025] Pretreatment of the original membrane: the original membrane (the original membrane is polytetrafluoroethylene (PTFE) membrane, polyvinylidene fluoride (PVDE) membrane, polyvinylidene fluoride (PVDF) membrane, polysulfone (PS), polyethersulfone (PES) membranes, etc., and not limited to hollow fiber membranes, flat membranes, and tubular membranes) plasma treatment is performed under a nitrogen atmosphere to generate relatively stable free radicals and active sites on the membrane surface, wherein the time of plasma treatment 1 minute to 10 minutes, the nitrogen flow rate is 0.1L min -1 to 0.5L·min -1 ;

[0026] Graft polymerization on the surface of the membrane: put the pretreated membrane into an acrylic acid solution for static immersion for a period of time, then take it out and place it between two glass plates, and carry out thermal polymerization in a va...

Embodiment 1

[0040] Embodiment 1: Preparation method of precursor solution and organic ligand solution

[0041] Preparation of zinc nitrate solution: dissolve 0.372g of zinc nitrate hexahydrate in 4ml of water, and mix in an ultrasonic bath for 5 minutes; prepare dimethylimidazole solution: dissolve 4.1g of dimethylimidazole in 40ml of pure water, and mix evenly in an ultrasonic bath for 5 minutes.

Embodiment 2

[0042] Embodiment 2: Coordinated self-assembly and in-situ growth of nanozymes on the surface of polytetrafluoroethylene membranes

[0043] Will figure 1 The prepared PAA / PTFE polytetrafluoroethylene grafted membrane was immersed in 4ml of zinc nitrate solution with a concentration of 0.3125mol / L for 20 minutes to utilize the metal ion Zn in the ZIF-8 precursor 2+ Coordination with carboxyl groups on the membrane surface assembles to the surface of the grafted membrane. Add 40ml of dimethylimidazole solution with a concentration of 1.25mol / L to the precursor solution, then immediately add 4ml of horseradish peroxidase solution with a concentration of 7.5mg / mL, co-precipitate at 200rpm for 20 minutes, and then take out the membrane.

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Abstract

The invention provides a method for in-situ preparation of a nano-enzyme membrane. The method comprises pretreating an original membrane, carrying out grafting polymerization on the membrane surface,carrying out coordination self-assembling and in-situ growth on the nano-enzyme on the membrane surface, immersing the membrane grafted with polyacrylic acid in a MOFs precursor solution for some timeso that metal ions in the precursor solution and the carboxyl group of polyacrylic acid are assembled to the membrane surface through coordination, adding an organic ligand solution and an enzyme liquid into the precursor solution so that the enzyme is wrapped by the MOFs skeleton structure and the in-situ growth of the nano-enzyme on the membrane surface is realized, and carrying out after-treatment on the nano-enzyme membrane so that the nano-enzyme membrane with the nano-enzyme is obtained. The method greatly improves enzyme activity and stability, improves the loading rate of the enzyme on the membrane, reduces the water contact angle of the original membrane, realizes high water flux and provides the polymer membrane with pollution resistance, biocatalysis, photocatalytic degradationof pollutants and other functions.

Description

technical field [0001] The invention relates to a method for preparing nano-enzyme film in situ. Background technique [0002] Enzyme is a kind of high-efficiency biocatalyst, which has the advantages of high catalytic efficiency, strong specificity, mild reaction conditions, and no pollution. However, free enzymes have the disadvantages of poor stability and difficult recovery, which limit their wide application in the field of water treatment. The emergence of enzyme immobilization technology has opened up broad prospects for the application of enzymes and further promoted the application of enzymes in the field of water treatment. Using porous separation membranes as carriers, enzyme molecules can be immobilized on the membrane surface or in the pores to prepare enzyme membranes with excellent separation performance and biochemical properties. Therefore, the preparation of enzyme membranes has become a research hotspot. At present, researchers at home and abroad mainly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08C08J7/18C08L27/18
CPCC08J7/18C08J2327/18C08J2433/02C12N9/0065C12N11/08C12Y111/01007
Inventor 迟莉娜蒋政王欣泽解婷婷刘瑞婷张博宇
Owner 深圳市新纳捷科技有限公司
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