A method for immobilizing phospholipase with cellulose acetate/polypropylene composite membrane

A technology of immobilizing phospholipase and cellulose acetate, which is applied in the direction of immobilization on/in the organic carrier, and can solve the problem of unsatisfactory immobilized phospholipase effect

Inactive Publication Date: 2011-12-21
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] The present invention aims at the unsatisfactory effect of immobilizing phospholipase by the single-membrane m

Method used

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0007] Specific embodiment one: immobilize phospholipase A with cellulose acetate / polypropylene composite membrane 1 The method is realized through the following steps: 1. Preparation of composite membrane: Dissolve 5 g of cellulose acetate in 100 mL of acetone, and after dissolving into a uniform membrane liquid, take the polypropylene membrane prepared before the experiment and soak it in the solution of cellulose acetate In the acetone solution of phospholipase, a film is naturally formed, and the cellulose acetate / polypropylene composite film is rinsed with deionized water for several times and dried for later use; 2. Immobilization of phospholipase: take several parts of 1 cm 2 The cellulose acetate / polypropylene composite membrane was placed in a Erlenmeyer flask, and a certain amount of enzyme solution and disodium hydrogen phosphate / citric acid buffer solution with a pH of 5.0 were respectively added, and stirred and adsorbed at a constant temperature for a certain peri...

specific Embodiment approach 2

[0008] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 4, the cellulose acetate / polypropylene composite membrane and the enzyme solution are adsorbed and immobilized for phospholipase under the condition of adsorption time of 1.5-5.5 hours. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0009] Specific embodiment three: the difference between this embodiment and specific embodiment one is that in step four, the cellulose acetate / polypropylene composite membrane and the enzyme liquid are adsorbed under the conditions of 2.5 to 4.5 hours, and the concentration of the enzyme liquid is 0.03 to 0.07 g / mL. Other steps are the same as in the first embodiment.

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Abstract

The invention provides a cellulose acetate/polypropylene composite film prepared by using cellulose acetate and polypropylene film as materials, adopts an adsorption-crosslinking immobilization method, and uses the composite film to immobilize phospholipase. The effects of different immobilization conditions such as adsorption time, enzyme solution concentration, crosslinking agent concentration, crosslinking time and crosslinking temperature on the immobilization efficiency and catalytic effect of phospholipase were studied, and the enzymatic properties of the immobilized enzyme membrane were investigated. discuss. The advantage of using cellulose acetate/polypropylene composite membrane to immobilize phospholipase was further explained by the SEM images of the immobilized enzyme membrane. According to the adsorption characteristics of the composite membrane in the present invention, the immobilized phospholipase improves the activity and stability of the enzyme in the reaction system, regulates and controls the activity and selectivity of the enzyme, thereby facilitating the recovery of the enzyme and the production of the product, and finally obtains The immobilized enzyme activity is 4.6U/cm2.

Description

Technical field: [0001] The invention relates to a method for immobilizing phospholipase. Background technique: [0002] Phospholipases are widely used in phospholipid modification and oil refining, but free phospholipase A 1 The price is expensive, and it is not easy to recycle, and the conditions for catalyzing hydrolysis are harsh; compared with free enzymes, immobilized enzymes maintain their high-efficiency, specific and mild enzyme-catalyzed reaction characteristics, while also showing high storage stability, easy separation and recovery, and It has the advantages of repeated use, continuous and controllable operation, and simple process. When the enzyme exists in a free state, some defects such as poor stability and short service life have not been improved. Immobilizing the enzyme on the separation membrane can effectively avoid the above shortcomings. In addition, when the enzyme exists in a free state, the unfavorable adsorption on the surface of the enzyme membr...

Claims

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Application Information

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IPC IPC(8): C12N11/12C12N11/08
Inventor 于殿宇杜晶王立琦梁单琼王妍王雪李越张佳宁时敏陈晓慧李志平奚会松宋云花周旋
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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