Method for preparing biological diesel oil from immobilized lipase-porous membrane biological reactor

A technology of immobilized lipase and bioreactor, which is applied in the field of membrane separation, can solve the problems of no process and practical application, and achieve the effects of avoiding solvent cleaning and batch feeding, high enzyme activity and stability, and simple process

Inactive Publication Date: 2008-09-17
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there has been no report on the use of immobilized enzyme polymer separation membranes for biodiesel production, and there is no specific implementation process an...

Method used

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  • Method for preparing biological diesel oil from immobilized lipase-porous membrane biological reactor

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Effect test

Embodiment 1

[0020] A polyacrylonitrile porous membrane (molecular weight cut-off 10kDa) immobilized lipase (from Candida antarctica) was made into a membrane module, which was connected to an enzyme-membrane bioreactor. Inject the mixture of 2.5L fish oil and 2.5L methanol into the raw material storage tank 1, and go through the pump 2 to the shell side of the membrane module 3 at a flow rate of 5L / h; the water flows from the storage tank 6 through the pump 7 to the tube side of the membrane module 3 at a flow rate of 2L / h. The yield of biodiesel is about 91% after 4h circulation.

Embodiment 2

[0022] A polysulfone porous membrane (molecular weight cut-off 1 kDa) immobilized lipase (derived from Rhizomucor miehei) was made into a membrane module, which was connected to an enzyme-membrane bioreactor. The mixture of 2L lard and 3L ethanol is injected into the raw material storage tank 1, and the membrane module 3 shell passes through the pump 2, and the flow rate is 5L / h; the water flows from the storage tank 6 through the pump 7, and the membrane module 3 tube pass, the flow rate is 10L / h h. The yield of biodiesel is about 93% after 4h circulation.

Embodiment 3

[0024] A polysulfone porous membrane (molecular weight cut-off 10 kDa) immobilized lipase (derived from Thermomyces lanuginosus) was made into a membrane module, which was connected to an enzyme-membrane bioreactor. The mixture of 2L castor oil and 3L methanol is injected into the raw material storage tank 1, and the membrane module 3 shell side is passed through the pump 2, and the flow rate is 5L / h; the water is passed from the storage tank 6 through the pump 7 and the membrane module 3 tube side, and the flow rate is 2L / h. h. The yield of biodiesel is about 92% after 4h circulation.

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Abstract

The invention discloses a method for preparing biodiesel through an immobilized lipase-porous membrane bioreactor. The hollow fiber porous membrane modules of immobilized lipase are assembled into an enzyme-membrane bioreactor, the admixture of grease and lower alcohol passes through the shell pass of the membrane modules, water passes through the tube pass of the membrane modules, after the grease and the lower alcohol are circularly reacted for a period of time under the catalytic action of the immobilized lipase, the grease and the lower alcohol can be transformed into the biodiesel. Because the method of the invention adopts the lipase-porous membrane as the medium between the grease phrase and the water phase, not only the on line byproduct glycerol separating can be performed, but also necessary water can be actively supplied to the immobilized lipase, therefore high enzyme activity and stability can be maintained, and biodiesel can be efficiently prepared, simultaneously solvent cleaning and batch charging are avoided, and the method has the advantages that the technology is simple, the continuous operation can be performed, the cost is lower, and the method meet the green chemical development trend.

Description

technical field [0001] The invention belongs to the technical field of membrane separation. In particular, it relates to a method for preparing biodiesel by using a polymer porous membrane with immobilized lipase. Background technique [0002] As an environmentally friendly fuel oil, biodiesel has been highly valued at home and abroad. At present, the methods for preparing biodiesel mainly include chemical synthesis method, biological enzyme method, supercritical fluid method and engineering microalgae method, etc. Among them, the preparation of biodiesel by immobilized enzyme has the advantages of high efficiency, low energy consumption (mild reaction conditions), good selectivity, small amount of alcohol, less by-products, and environmental friendliness (no need for waste liquid treatment). However, the immobilized enzyme method also has problems such as the adsorption of by-product glycerol on the surface of the enzyme molecule, which reduces the stability of the enzyme...

Claims

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Application Information

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IPC IPC(8): C10G3/00C12M1/40
CPCC12M29/02C12M29/04C12M21/12C12M21/18Y02P30/20
Inventor 徐志康万灵书黄小军
Owner ZHEJIANG UNIV
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