Ginseng peptide liquid composition with sleep improving function and preparation method thereof
By integrating dynamic countercurrent extraction, membrane purification, enzyme membrane reactor, and nanotechnology, the prepared ginseng peptide liquid composition solves the problems of poor absorption, unpleasant taste, and insufficient effect of traditional sleep improvement products, and provides a safe and efficient sleep improvement solution.
Patent Information
- Application Number
- CN202511266327.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-05
- Publication Date
- 2025-11-28
AI Technical Summary
Existing chemical drugs and traditional natural supplements have problems such as dependence, side effects, low absorption efficiency and limited effectiveness in improving sleep. Traditional Chinese medicine preparations have disadvantages such as poor taste, low concentration of active ingredients and slow absorption.
A ginseng peptide liquid composition containing ginseng nanopeptides, poria cocos peptides, walnut peptides, jujube seed extract, and passion fruit nanoparticles was prepared using dynamic countercurrent extraction, membrane purification, enzyme membrane reactor, nano-sizing, and non-thermal sterilization technologies. This composition improves sleep through multi-target synergistic effects.
It achieves efficient, safe, and well-absorbed sleep improvement effects. The product has a mellow and stable taste, retains the bioactivity and freshness of its ingredients, avoids organic solvent residues and high-temperature damage, and significantly improves bioavailability.
Smart Images
Figure CN121014864A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to a ginseng peptide liquid composition with sleep improvement function and a preparation method thereof, and belongs to the technical field of health food. BACKGROUND
[0002] Currently, the main solutions for sleep disorders include chemical drugs (such as benzodiazepines) and natural supplements (such as melatonin). However, chemical drugs are prone to dependence and side effects, while traditional natural supplements have low absorption efficiency and limited effects. The concept of "medicinal food homology" in traditional Chinese medicine theory provides a new way to improve sleep. For example, the classic prescription "Ganmai Dazao Decoction" has the effect of nourishing the heart and calming the mind. However, traditional water decoction has the disadvantages of poor taste, low concentration of active ingredients, and slow absorption.
[0003] In recent years, the progress of modern food processing technologies such as bioenzymatic hydrolysis, membrane separation, and nanotechnology has made it possible to efficiently extract and enrich active ingredients from traditional raw materials and improve their bioavailability. Therefore, there is an urgent need to develop a sleep improvement product that combines traditional Chinese medicine theory with modern food processing technology, with significant efficacy, high safety, and good absorption. SUMMARY
[0004] The technical problem to be solved by the present application is to provide a ginseng peptide liquid composition with sleep improvement function to overcome the shortcomings of the prior art.
[0005] Another object of the present application is to provide a method for preparing the above-mentioned composition, which integrates dynamic countercurrent extraction, membrane refining, enzyme membrane reactor, nanotechnology, and non-thermal sterilization, etc. The method has the advantages of high efficiency, precision, and maximum retention of active ingredients.
[0006] The present application is realized by the following scheme: a ginseng peptide liquid composition with sleep improvement function, which is composed of the following raw materials by weight:
[0007] Water-boiled extract of jujube, wheat, and licorice 15-20 parts;
[0008] Ginseng nanopeptide 0.01-0.05 parts;
[0009] Poria peptide 0.01-0.05 parts;
[0010] Walnut peptide 0.01-0.05 parts;
[0011] Semen ziziphi spinosae extract 0.35-0.5 parts;
[0012] Passiflora nanometer powder 0.5-1 parts;
[0013] The ginseng nanopeptide is a ginseng peptide obtained by ultra-nano processing technology, with an average particle size of ≤200 nm; and the passion fruit nanometer powder is prepared by low-temperature ultra-fine grinding technology, with an average particle size D90≤15 μm.
[0014] The extract of Suanzaoren is prepared by ultrasonic-assisted deep eutectic solvent (DES) extraction technology, and the deep eutectic solvent is composed of choline chloride and lactic acid at a molar ratio of 1:2.
[0015] It also contains 0.1-0.3 parts of gamma-aminobutyric acid (GABA) embedded by phospholipid complex.
[0016] A preparation method of a ginseng peptide liquid composition with improved sleep function, comprising the following steps:
[0017] s1, dynamic countercurrent extraction: according to the ratio, jujube, wheat and licorice raw materials are taken, washed and sliced, then dynamic countercurrent extraction unit is used to continuously extract under the condition of 85-95℃ and solid-liquid ratio of 1:10-1:12(g / mL), and the extract is collected;
[0018] s2, precise refining by membrane method: the extract obtained in s1 is first passed through a 100 nm ceramic microfiltration membrane to remove macromolecular impurities and suspended particles, then an ultrafiltration membrane with a molecular weight cut-off of 5000 Da is used to retain the target active ingredients, and finally a roll-type nanofiltration membrane is used to concentrate at a low temperature under the condition of 50-60℃ and operating pressure of 3.5-4.5 MPa, to obtain a clear refined extract with a relative density of 1.10-1.20;
[0019] s3, enzyme-membrane bioreactor synergistic enzymolysis: the refined extract obtained in s2 is pumped into an enzyme-membrane bioreactor equipped with immobilized complex protease, and the circulating enzymolysis is carried out at 45-50℃ and pH 6.0-6.5 for 40-60 minutes; the 10 kDa ultrafiltration membrane built-in the reactor separates the small molecular peptide fragments in real time;
[0020] s4, nanofication and stabilization: ginseng peptide and passion fruit powder are respectively subjected to ultra-micro nanofication treatment by nano impact mill; under low speed stirring, ginseng nanopeptide, tremella peptide, walnut peptide, Suanzaoren extract and passion fruit nanometer powder are sequentially added into the enzymolysis liquid obtained in s3 and mixed uniformly; then 0.05-0.1 parts of gum arabic is added as a stabilizer, and the homogeneous liquid is subjected to homogenization by a high-pressure homogenizer under the condition of 150-200 MPa to form a stable system;
[0021] s5, non-thermal sterilization and sterile filling: the homogeneous liquid obtained in s4 is sterilized by ultra-high pressure processing technology or low-temperature ultraviolet-pulsed light combined sterilization technology under the protection of inert gas, and finally sterile cold filling is carried out in a class A clean environment, to obtain the ginseng peptide liquid composition.
[0022] The conditions of the dynamic countercurrent extraction in s1 are as follows: the extraction times are three levels, the extraction temperatures of the three levels are 90 DEG C, 85 DEG C and 80 DEG C respectively, and the total extraction time is 2.5 hours.
[0023] The immobilized complex protease in s3 is a mixture of bromelain and papain immobilized on magnetic Fe3O4@SiO2 nano-carriers by covalent binding method, and the enzyme activity retention rate is greater than or equal to 85%.
[0024] The operating pressure difference of the enzyme-membrane bioreactor in s3 is maintained at 0.2-0.4 MPa, and the membrane surface flow rate is controlled at 2.5-3.5 m / s, so as to reduce the membrane pollution.
[0025] The conditions of the ultra-high pressure processing in s5 are as follows: the pressure is 500-600 MPa, the pressure maintaining time is 3-5 minutes, and the temperature is 20-25 DEG C; the conditions of the ultraviolet-pulsed light combined sterilization are as follows: the ultraviolet wavelength is 254 nm, the pulsed light intensity is 0.5-1.0 J / cm 2 , the processing time is 30-60 seconds, the whole process is protected by nitrogen, and the temperature is controlled below 30 DEG C.
[0026] The sterile cold filling in s5 is carried out under the protection of nitrogen, and the dissolved oxygen content of the product after filling is controlled below 0.5 ppm.
[0027] The beneficial effects of the present application are as follows:
[0028] 1. The present application takes the extract of the classical prescription of traditional Chinese medicine "Ganmai Dazao Decoction" as the basis, and compounding a variety of small molecule peptides, jujube seed extract and passionflower, which play a synergistic role in improving sleep and relieving anxiety through multiple targets and multiple pathways.
[0029] 2. The key ingredients (ginseng peptide, passionflower) of the present application are treated by nanofication, the particle size is significantly reduced, the solubility and intestinal absorption rate are greatly improved, and the biological activity is enhanced.
[0030] 3. The present application adopts the combination process of dynamic countercurrent extraction, membrane separation technology, enzyme membrane reactor, realizes efficient, low temperature and precise extraction, separation and transformation, avoids the damage of high temperature to the heat-sensitive components, and ensures the high quality and activity of the product.
[0031] 4. The present application adopts physical and biological technology throughout the process, avoids the residual of organic solvents, and finally adopts non-thermal sterilization technology (HPP or ultraviolet-pulsed light), which effectively sterilizes and maximally retains the biological activity and fresh taste of the components without adding preservatives
[0032] 5、The film technology effectively removes macromolecular impurities and astringent components, and cooperates with the nano homogenization technology, so that the product has a mellow taste, is stable and uniform, has no precipitation, and is easy for consumers to accept. BRIEF DESCRIPTION OF DRAWINGS
[0033] Figure 1 A ginseng peptide liquid composition with improved sleep function and a preparation method thereof.
[0034] Figure 2 A sleep incidence result chart of a ginseng peptide liquid composition with improved sleep function and a preparation method thereof.
[0035] Figure 3 A sleep time result chart of a ginseng peptide liquid composition with improved sleep function and a preparation method thereof.
[0036] In the figure: 100 is a countercurrent extraction unit, 200 is a ceramic microfiltration membrane, 300 is an ultrafiltration membrane, 400 is a roll-type nanofiltration membrane, 500 is an enzyme-membrane bioreactor, 600 is a high-pressure homogenizer, and 700 is an ultraviolet-pulsed light combined sterilization device. DETAILED DESCRIPTION
[0037] The application will be further described below Figure 1 but the scope of protection of the application is not limited to the described content.
[0038] In order to be clear, not all features of the actual embodiments are described, in the following description, well-known functions and structures are not described in detail, because they will make the application confused by unnecessary details, it should be considered that in the development of any actual embodiment, a large number of implementation details must be made to achieve the specific goals of the developers, such as changing from one embodiment to another according to the relevant system or relevant business restrictions, in addition, it should be considered that such development work can be complex and time-consuming, but for those skilled in the art, it is only routine work.
[0039] Example 1:
[0040] A ginseng peptide liquid composition, which is composed of the following raw materials by weight:
[0041] Water-boiling extraction liquid of jujube, wheat and licorice 18 parts, ginseng nano-peptide (average particle size 180 nm) 0.03 parts, tremella peptide 0.03 parts, walnut peptide 0.03 parts, extract of zizyphus jujuba mill (spinosin content 8.5%) 0.4 parts, passion fruit nano powder (D90=12 μm) 0.8 parts, and GABA embedded by phospholipid 0.2 parts.
[0042] The preparation method comprises the following steps:
[0043] s1, dynamic countercurrent extraction: according to the ratio, take the jujube, wheat, licorice raw materials, after cleaning and slicing, use dynamic countercurrent extraction unit (100) to carry out continuous countercurrent extraction under the condition of 85-95℃, solid-liquid ratio 1:10-1:12 (g / mL), and collect the extraction liquid;
[0044] s2, membrane method combined with precise refining: the extraction liquid obtained in s1 is first subjected to 100 nm ceramic microfiltration membrane (200) to remove macromolecular impurities and suspended particles, then a 5000 Da ultrafiltration membrane (300) is used to retain the target active ingredients, and finally a roll-type nanofiltration membrane (400) is used to concentrate at a low temperature under the condition of 50-60℃, operating pressure 3.5-4.5 MPa, to obtain a clear refined extraction liquid with a relative density of 1.10-1.20;
[0045] s3, enzyme-membrane bioreactor synergistic enzymolysis: the refined extraction liquid obtained in s2 is pumped into an enzyme-membrane bioreactor (500) equipped with immobilized complex protease, and subjected to cyclic enzymolysis at 45-50℃, pH 6.0-6.5 for 40-60 minutes; a 10 kDa ultrafiltration membrane built-in the reactor separates small molecule peptides in real time;
[0046] s4, nanofication and complexing and stabilizing: ginseng peptides and passion fruit powder are subjected to ultramicro-nanofication treatment by nanoshock mill; under low-speed stirring, ginseng nanopeptides, tremella peptides, walnut peptides, jujube kernel extract, and passion fruit nanometer powder are sequentially added to the enzymolysis liquid obtained in s3, and mixed uniformly; then 0.05-0.1 parts of gum arabic is added as a stabilizer, and the mixture is subjected to homogenization by a high-pressure homogenizer (600) under the condition of 150-200 MPa to form a stable system;
[0047] s5, non-thermal sterilization and sterile filling: the homogenized liquid obtained in s4 is sterilized by ultrahigh pressure processing technology or a low-temperature ultraviolet-pulsed light combined sterilization device (700) under the protection of inert gas, and finally sterile cold filling is carried out in a class A clean environment to obtain the ginseng peptide liquid composition. Example 2:
[0048] A ginseng peptide liquid composition is composed of the following raw materials by weight:
[0049] Water-boiled extraction liquid of jujube, wheat, and licorice 20 parts, ginseng nanopeptides (average particle size 150 nm) 0.05 parts, tremella peptides 0.01 parts, walnut peptides 0.05 parts, jujube kernel extract (spinosin content 9.2%) 0.35 parts, passion fruit nanometer powder (D90=10 μm) 1 part.
[0050] The preparation method is similar to that of example 1, except that:
[0051] In step (1), the proportions of jujube, wheat and licorice are 4:8:1, and the extraction temperatures of each stage are 95℃, 90℃ and 85℃, respectively.
[0052] In step (3), the enzymolysis pH is 6.5, the temperature is 50℃, and the enzyme addition amount is 0.3‰.
[0053] In step (5), ultra-high pressure HPP sterilization (pressure 550MPa, pressure maintaining time 4 minutes, temperature 22℃) is adopted.
[0054] In this example, GABA is not added.
[0055] Example 3:
[0056] A ginseng peptide liquid composition is composed of the following raw materials by weight:
[0057] 15 parts of water-extracted jujube, wheat and licorice extract, 0.01 parts of ginseng nano-peptide (average particle size 200nm), 0.05 parts of poria peptide, 0.01 parts of walnut peptide, 0.5 parts of semen ziziphi spinosae extract, 0.5 parts of passion fruit nano powder (D90=15μm), and 0.1 parts of GABA embedded by phospholipid.
[0058] The preparation method is similar to that of Example 1, except that:
[0059] In step (1), the proportions of jujube, wheat and licorice are 2:10:1, and the extraction temperatures of each stage are 90℃, 85℃ and 80℃, respectively.
[0060] In step (3), the enzymolysis pH is 6.0, the temperature is 45℃, the enzyme addition amount is 0.1‰, and the enzymolysis time is 40 minutes.
[0061] In step (4), the high-pressure homogenization pressure is 150MPa, and the gum arabic addition amount is 0.05 parts.
[0062] Comparative Example 1 (traditional water decoction process):
[0063] The formula is the same as that of Example 1, but the preparation method is changed to: licorice, wheat and jujube are decocted twice with water, each time for 1.5 hours, the filtrates are combined and concentrated to an appropriate amount. The other ginseng peptides (non-nano), ordinary passion fruit powder and other raw materials are directly added, stirred and dissolved, and then sterilized at high temperature (121℃) for 15 minutes.
[0064] Comparative Example 2 (lacking nanoization step):
[0065] The formula is the same as that of Example 1, but the ginseng peptides and passion fruit powder are not subjected to nanoization treatment and are directly added in ordinary form.
[0066] Comparative Example 3 (high-temperature sterilization):
[0067] The preparation process is the same as Example 1 until step (4), but the final sterilization uses the traditional 121°C, 15-minute heat pressure sterilization method.
[0068] Experimental Example: Product Property and Sleep Improvement Function Test
[0069] 1. Product Property Evaluation: The products of Example 1 and Comparative Examples 1-3 were evaluated. The product of Example 1 was a uniform light brown opalescent liquid, with a mellow taste, a moderate sweet and sour taste, no traditional Chinese medicine bitter taste, no precipitation or layering after 6 months of storage at room temperature. The product of Comparative Example 1 had a noticeable traditional Chinese medicine taste and astringency, and produced a small amount of precipitation after long storage. The product of Comparative Example 3 had a darkened color (Maillard reaction) and a slight burnt taste due to high temperature sterilization.
[0070] 2. Sleep Improvement Function Animal Test:
[0071] Method: A number of SPF grade ICR mice were selected and divided into the Example 1 group, the Comparative Examples 1-3 groups. Each group was given intraperitoneal injections of para-chlorophenylalanine (PCPA) to establish an insomnia model. After the model was successfully established, each group was given a corresponding test substance by gavage for 28 consecutive days. After the last administration, a sodium pentobarbital sleep test was performed, and the threshold dose sleep incidence and sleep time were recorded.
[0072] Results:
[0073] Compared with the model control group, each administration group showed a certain sleep improvement effect;
[0074] The Example 1 group had the most significant effect, and could significantly shorten the sleep latency, prolong the sleep time, and the sleep incidence was 100%;
[0075] The Comparative Example 1 group (traditional process) had a significantly weaker effect than the Example 1 group, proving that the modern combined process is crucial for improving the extraction efficiency and bioavailability of the active ingredients;
[0076] The Comparative Example 2 group (non-nano) had an effect between Example 1 and Comparative Example 1, but was significantly different from Example 1, proving the key role of nanometerization in improving the bioavailability of the active ingredients;
[0077] The Comparative Example 3 group (high temperature sterilization) had the worst effect, proving that high temperature sterilization destroyed the heat-sensitive active ingredients, resulting in product failure.
[0078] The Shen Pei liquid composition and the preparation method thereof successfully combine traditional Chinese medicine theory with modern food high technology (dynamic countercurrent extraction, membrane separation, enzyme membrane reactor, nanometer, non-thermal sterilization), through the synergistic effect of multiple components and multiple targets, and the nanotechnology of greatly improving bioavailability, creatively solve the problems of poor absorption, poor taste, insufficient efficacy and the like of traditional products, and provide a safe, efficient and excellent taste modern health food solution for improving sleep.
[0079] Although the technical solutions of the present application have been described and listed in detail, it should be understood that modifications to the above examples or the adoption of equivalent alternatives will be apparent to those skilled in the art, and these modifications or improvements made without departing from the spirit of the present application are within the scope of the present application.
Claims
1. A peptide liquid composition with sleep-improving function, characterized in that, It consists of the following raw materials in parts by weight: 15-20 parts of a water-boiled extract of jujube, wheat, and licorice; Ginseng nanopeptide 0.01-0.05 parts; Poria cocos peptide 0.01-0.05 parts; Walnut peptides 0.01-0.05 parts; Ziziphus jujuba seed extract, 0.35-0.5 parts; Passion fruit nanoparticles 0.5-1 part; The ginseng nanopeptide is a ginseng peptide obtained by ultra-micro nanotechnology, with an average particle size ≤200nm; the passion fruit nanoparticle is prepared by low-temperature ultra-micro pulverization technology, with an average particle size D90 ≤15μm.
2. The ginseng peptide liquid composition with sleep-improving function according to claim 1, characterized in that, The active ingredient of the jujube seed extract is jujube seed saponin. The jujube seed extract is prepared using ultrasound-assisted eutectic solvent (DES) extraction technology. The eutectic solvent is composed of choline chloride and lactic acid in a molar ratio of 1:
2.
3. The ginseng peptide liquid composition with sleep-improving function according to claim 1, characterized in that, It also contains 0.1-0.3 parts of γ-aminobutyric acid (GABA) encapsulated in a phospholipid complex.
4. A method for preparing the peptide liquid composition according to any one of claims 1-3, characterized in that, Includes the following steps: s1. Dynamic countercurrent extraction: Take jujube, wheat and licorice raw materials according to the ratio, wash and slice them, and then use a dynamic countercurrent extraction unit (100) to carry out continuous countercurrent extraction at 85-95℃ and material-liquid ratio of 1:10-1:12 (g / mL), and collect the extract. s2, Membrane-based precision purification: The extract obtained in s1 is first passed through a 100nm ceramic microfiltration membrane (200) to remove macromolecular impurities and suspended particles, then an ultrafiltration membrane (300) with a molecular weight cutoff of 5000 Da is used to retain the target active ingredients, and finally a spiral wound nanofiltration membrane (400) is used for low-temperature concentration at 50-60℃ and an operating pressure of 3.5-4.5MPa to obtain a clear and purified extract with a relative density of 1.10-1.20; s3, Enzyme-membrane bioreactor synergistic enzymatic hydrolysis: The purified extract obtained from s2 is pumped into an enzyme-membrane bioreactor (500) equipped with immobilized complex protease, and enzymatically hydrolyzed for 40-60 minutes at 45-50℃ and pH 6.0-6.5; small molecule peptides are separated in real time by the 10kDa ultrafiltration membrane built into the reactor. s4. Nano-compounding and stabilization: Ginseng peptides and passion fruit powder were subjected to ultra-micro and nano-sized treatment using a nano-impact mill. Under low-speed stirring, ginseng nano-peptides, poria cocos peptides, walnut peptides, jujube seed extract, and passion fruit nano-peptides were added sequentially to the enzymatic hydrolysate obtained in s3 and mixed evenly. Subsequently, 0.05-0.1 parts of gum arabic were added as a stabilizer, and homogenization was carried out using a high-pressure homogenizer (600) at 150-200 MPa to form a stable system. s5. Non-thermal sterilization and aseptic filling: The homogenized liquid obtained in s4 is sterilized by ultra-high pressure processing technology or by a low-temperature ultraviolet-pulse light combined sterilization device (700) under inert gas protection. Finally, aseptic cold filling is performed in a Class A clean environment to obtain the ginseng peptide liquid composition.
5. The method for preparing a ginseng peptide liquid composition with sleep-improving function according to claim 1, characterized in that, The conditions for dynamic countercurrent extraction described in s1 are: three extraction stages, extraction temperatures of 90℃, 85℃, and 80℃ for each stage, and a total extraction time of 2.5 hours.
6. The method for preparing a ginseng peptide liquid composition with sleep-improving function according to claim 1, characterized in that, The immobilized complex protease described in s3 is a mixture of bromelain and papain immobilized on a magnetic Fe3O4@SiO2 nanocarrier via covalent bonding, with an enzyme activity retention rate of ≥85%.
7. The method for preparing a ginseng peptide liquid composition with sleep-improving function according to claim 1, characterized in that, The operating pressure differential of the enzyme-membrane bioreactor (500) described in s3 is maintained at 0.2-0.4 MPa, and the membrane surface flow velocity is controlled at 2.5-3.5 m / s to reduce membrane fouling.
8. The method for preparing a ginseng peptide liquid composition with sleep-improving function according to claim 1, characterized in that, The ultra-high pressure processing conditions described in s5 are: pressure 500-600 MPa, holding time 3-5 minutes, and temperature 20-25℃; the operating conditions of the ultraviolet-pulse light combined sterilization device (700) are: ultraviolet wavelength 254 nm, and pulse light intensity 0.5-1.0 J / cm². 2 The processing time is 30-60 seconds, with nitrogen protection throughout and temperature controlled below 30℃.
9. The method for preparing a ginseng peptide liquid composition with sleep-improving function according to claim 1, characterized in that, The aseptic cold filling described in s5 is carried out under nitrogen protection, and the dissolved oxygen content of the product after filling is controlled below 0.5 ppm.