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Nanosilver modified titanium dioxide nanorod array enzyme electrode and preparation method and application thereof

A nanocolumn array and titanium dioxide technology, applied in the field of biosensors, can solve the problems affecting the immobilization efficiency of glucose oxidase, large specific surface area of ​​nanotube arrays, low bioelectrocatalytic activity, etc., to achieve easy large-scale production and improve electrocatalytic activity , stable effect

Active Publication Date: 2018-11-06
XUZHOU MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the effect of surface tension, neither the fixation of glucose oxidase on the electrode surface nor the diffusion of electrolyte solution to the electrode surface, it is difficult to reach TiO. 2 inside the nanotubes, and thus cannot fully exploit the TiO 2 The advantage of the large specific surface area of ​​the nanotube array affects the improvement of the immobilization efficiency of glucose oxidase
In addition, simply by TiO 2 Enzyme electrodes composed of nanomaterials often show low bioelectrocatalytic activity

Method used

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  • Nanosilver modified titanium dioxide nanorod array enzyme electrode and preparation method and application thereof
  • Nanosilver modified titanium dioxide nanorod array enzyme electrode and preparation method and application thereof
  • Nanosilver modified titanium dioxide nanorod array enzyme electrode and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0045] This embodiment provides a preparation method of nano-silver modified titanium dioxide nanocolumn array enzyme electrode, such as figure 1 As shown, the specific steps of the method are as follows:

[0046] (1) Using carbon paper as the base material, growing TiO on the carbon paper 2 nanopillar array

[0047] Cut the carbon paper into small pieces of length×width×thickness: 50mm×20mm×0.2mm, wash them ultrasonically with acetone and deionized water for 10 minutes, and then dry them at 80°C for later use. figure 1 Shown in A.

[0048] Take 20mL of butyl titanate in a 200mL beaker, slowly add 100mL of absolute ethanol while stirring, then dropwise add 0.2mL of glacial acetic acid, and keep stirring at room temperature until the reaction solution is milky white to prepare TiO 2 colloidal solution.

[0049] The treated spare carbon paper was impregnated in the above TiO 2 Colloidal solution for 5 minutes, taken out and dried at 80°C, then treated at 350°C for 20 minute...

Embodiment 2

[0071] In this example, pure carbon paper, the titanium dioxide nanocolumn array / carbon paper prepared in Comparative Example 2, and the nanosilver / titanium dioxide nanocolumn array / carbon paper prepared in Example 1 were used as working electrodes, and platinum sheet electrodes were used as counter electrodes. The saturated calomel electrode is used as a reference electrode, and at the working potential of -0.6V, the phosphate buffer with a pH value of 6.8 is used as the working electrolyte solution, and the current-time curve test is carried out to detect the effect of the three on H 2 o 2 Electrocatalytic performance, where H 2 o 2 The product of a reaction catalyzed by glucose oxidase.

[0072] The specific detection process is to add H once every 50s 2 o 2 solution, starting from the 100th s, increasing H each time 2 o 2 Concentration increased to 0.1mM (meaning 0.1mmol﹣L -1 , the same as below); starting from the 600th s, each increase in H 2 o 2 The concentrati...

Embodiment 3

[0076] In this example, the glucose oxidase / carbon paper prepared in Comparative Example 1, the glucose oxidase / titanium dioxide nanocolumn array / carbon paper prepared in Comparative Example 2, and the glucose oxidase / nano silver / titanium dioxide prepared in Example 1 were respectively used The nano-column array / carbon paper is used as the working electrode, the platinum sheet electrode is used as the counter electrode, the saturated calomel electrode is used as the reference electrode, and the phosphate buffer solution with a pH value of 6.8 is used as the working electrolyte solution to conduct the current-time curve test. Detect the electrocatalytic performance of the three on glucose. Among them, -0.5V is selected as the working potential when testing glucose oxidase / carbon paper enzyme electrode, and -0.5V is selected when testing glucose oxidase / nano-silver / titanium dioxide nanocolumn array / carbon paper enzyme electrode. 0.6V as the working potential.

[0077] The specif...

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Abstract

The invention relates to a nanosilver modified titanium dioxide nanorod array enzyme electrode and a preparation method and application thereof. According to the method, carbon paper is utilized as asubstrate material. The method comprises the steps: firstly, growing a TiO2 nanorod array on the carbon paper; then soaking the carbon paper which the TiO2 nanorod array grows on into silver sol for 3to 15 hours, taking out, utilizing deionized water to clean the carbon paper and drying to obtain nanosilver modified titanium dioxide nanorod array; finally, utilizing bovine serum albumin-glutaraldehyde as a cross-linking agent and utilizing an improved cross-linking method to fix glucose oxidase on the surface of the nanosilver modified titanium dioxide nanorod array to form the nanosilver modified titanium dioxide nanorod array enzyme electrode. The prepared nanosilver modified titanium dioxide nanorod array enzyme electrode disclosed by the invention has better electrocatalytic activityand can be well applied to a glucose biosensor or an enzyme biological fuel cell.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a nano-silver modified titanium dioxide nano-column array enzyme electrode and a preparation method and application thereof. Background technique [0002] The glucose oxidase electrode is a biosensor used to measure the glucose content in the solution to be tested. It is equipped with an immobilized enzyme membrane on the sensitive surface of the basic electrode. When the electrode is inserted into the solution to be tested, the glucose oxidase in the enzyme membrane will generate The catalytic reaction produces electrode active substances, and the response current changes with the concentration of active substances, thereby measuring the concentration of reactants or reaction products in the reaction catalyzed by the glucose oxidase. This glucose oxidase electrode biosensor has a simple structure, The advantages of high sensitivity, good selectivity, and real-time monitoring ...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/308G01N27/3272G01N27/3277G01N27/3278
Inventor 王永时梅林左凤梅徐凯庄银苹韩翠平吴长宇
Owner XUZHOU MEDICAL UNIV
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