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Novel enzyme electrode, and fuel cell comprising the enzyme electrode

An enzyme electrode, affinity technology, applied in the field of enzyme electrode, can solve the problems of output value limitation, enzyme and reaction substrate combination limitation, etc., to achieve the effect of improving electrical energy output and efficient redox reaction

Inactive Publication Date: 2011-04-13
SONY CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] However, in order to increase the output of a biofuel cell, it is necessary to obtain a high current while maintaining a high potential determined by an electron transfer medium, etc.; therefore, in the present case, combinations of enzymes and reaction substrates are limited, and the output value restricted

Method used

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  • Novel enzyme electrode, and fuel cell comprising the enzyme electrode
  • Novel enzyme electrode, and fuel cell comprising the enzyme electrode
  • Novel enzyme electrode, and fuel cell comprising the enzyme electrode

Examples

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Embodiment 1

[0073] In Example 1, a gene was prepared in which a codon encoding a specific amino acid residue was incorporated in a base sequence encoding the enzyme, thereby changing the enzyme that could be immobilized on the enzyme electrode according to the present invention.

[0074] Specifically, BOD as an example of an enzyme and a lysine residue as an amino acid residue that can be immobilized on the enzyme electrode according to the present invention are used to prepare a lysine residue-binding bilirubin oxidase (hereinafter referred to as "BOD") terminal gene.

[0075] First, the pMETαB vectors Lys4, Lys6, Lys8 and Lys10 incorporating four, six, eight and ten lysine residues, respectively, were prepared as BOD gene vectors, as shown in Table 1.

[0076] [Table 1]

[0077]

[0078]The gene containing the BOD gene (hereinafter referred to as "Lys4-BOD gene", "Lys6-BOD gene", "Lys8-BOD gene", "Lys10-BOD gene") is by PCR technology, using the pMETαB carrier Lys4 prepared above, ...

Embodiment 2

[0082] In Example 2, the plasmid prepared in Example 1 was transformed into Pichia Methanolica to investigate the presence or absence of BOD activity.

[0083] Using the induced cells of pMAD11, the Lys4 gene, Lys6 gene, Lys8 gene, and Lys10 gene excised with the restriction enzyme ASC1 were transformed into Pichia methanolica, respectively, and expanded in MD medium, and two colonies were obtained from each gene .

[0084] Colonies were grown with BMDY and transferred to BMMY to induce expression of BOD. To this end, ABTS (2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) was added, and the process of the color turning green was observed to check the expression of BOD activity .results in Figure 4 shown in . Notice, Figure 4 The text DM1 and DM2 in represent the BOD not bound to lysine residues.

[0085] like Figure 5 As shown, BOD activity was confirmed in all enzymes. Therefore, in Example 2, it was confirmed that the altered protein expressed by the gene incorpora...

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Abstract

Disclosed is an enzyme electrode in which an oxidation-reduction reaction proceeds by utilizing an enzyme as a catalyst. In the enzyme electrode, the enzyme immobilized thereon is so modified as to have increased affinity for a reaction substrate or an electron transfer mediator and / or an increased rate of reaction with the reaction substrate or the electron transfer mediator by adding or inserting at least one codon encoding a predetermined amino acid residue to a nucleotide sequence encoding the enzyme. On the enzyme electrode, an oxidation-reduction reaction can proceed with high efficiency. Therefore, an electric energy produced in the enzyme electrode can be output at a high level. For these reasons, the enzyme electrode can be used suitably in all types of fuel cells, biosensors and electronic devices.

Description

technical field [0001] The present invention relates to enzyme electrodes. More specifically, it relates to an enzyme electrode in which a redox reaction is performed using an enzyme that functions as a catalyst and which is improved to achieve higher output, and a fuel cell using the enzyme electrode to achieve higher output. Background technique [0002] In recent years, since electrons can be efficiently extracted from fuels such as glucose and ethanol, which do not easily react with conventional industrial catalysts, a fuel cell having an oxidoreductase immobilized on at least one of the negative electrode and the positive electrode serving as a catalyst (below termed "biofuel cell"), has been attracting attention as a next-generation fuel cell with high capacity and safety. [0003] use Figure 5 A general biofuel cell reaction scheme is described. exist Figure 5 In the shown biofuel cell using glucose as fuel, the oxidation of glucose is carried out at the negative ...

Claims

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Application Information

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IPC IPC(8): H01M8/16C12N9/02C12N15/00H01M4/90
CPCC12N9/0004Y02E60/527H01M8/16H01M4/9008Y02E60/50
Inventor 中川贵晶汲田英之后藤义夫酒井秀树角田正也
Owner SONY CORP
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