Method and reactor for removing organic matters by enzyme electrode coupled electroflocculation

Abstract

The invention provides a method and a reactor for removing organic matters by enzyme electrode coupled electroflocculation. In the method, the same electrochemical system in combination with an enzyme-modified electrode and electroflocculation synergistically removes organic matters in water; certain electrochemical parameters are controlled, so that polymerization reaction is performed on hydrogen peroxide generated by cathode reaction and an enzyme electrode-catalyzed organic matter; after the reaction, the organic product enters an anode chamber through a pump; the organic product and different electroflocculation hydrolyzates are precipitated and removed through compression of double electric layers, adsorption and electric neutralization, and precipitation netting capture and other coagulating / flocculating effects; and ultimately, the aim of removing the organic matters through cathode enzyme catalytic polymerization and anode electroflocculation synergistically is achieved. Themethod and the reactor have a strong selectivity for low-concentration toxic organic pollutants and a good effect in removing small-molecular hydrophilic organic matters; the enzyme adding amount is low; the cathode and the anode are fully used; the cost is reduced; the operation is simple; the applicable is wide; therefore, the method and the reactor is applicable to a variety of water and wastewater containing the organic matters, and contaminated water.

Description

technical field

[0001] The invention relates to a method for removing organic matter in water. Specifically, the invention proposes a method and a reactor for jointly removing organic matter in water by combining a peroxidase modified electrode and electrocoagulation in the same electrochemical system. Background technique

[0002] With the rapid advancement of my country's industrialization, the effluent of industrial wastewater still contains a large amount of low-concentration dissolved organic matter with various types and complex structures. For example, a certain wastewater is directly discharged after biochemical treatment, but it still contains polyphenols, polycyclic aromatic hydrocarbons, heterocyclic compounds, Low-concentration organic matter such as macromolecule humus. As a result, organic micro-pollution of water bodies in my country has become increasingly serious in recent years and has become an important hidden danger to water body safety.

[0003] Enzyme ...

Examples

Embodiment 1

[0036] The effective volume of the cathode and anode chambers of the reactor is 40mL. A self-made enzyme-modified electrode was used as the cathode, horseradish peroxidase was used as the enzyme, and the amount of enzyme added was 10U / cm 2 , the substrate electrode is graphite, the air flow rate of the aeration device is 0.1L / min, the anode is an aluminum plate, the distance between the cathode and the anode is 1mm, the saturated calomel electrode is used as the reference electrode, and the DC stabilized current power supply controls the cathode potential as -0.2V. The bisphenol A solution (concentration 100mg / L) enters the enzyme electrode coupled electrocoagulation reactor from the water inlet, and the residence time is 5 minutes. The removal rate of bisphenol A in the effluent treated by the system is 90%. Under the same conditions, the removal rate of bisphenol A was only 25% using the graphite electrode without enzyme immobilization. It shows that the synergistic effect...

Embodiment 2

[0038] The effective volume of the cathode and anode compartments of the reactor is 4L. A self-made enzyme-modified electrode is used as the cathode, the enzyme is lignin peroxidase, and the amount of enzyme added is 1000U / cm 2 , the matrix electrode is activated carbon, the air flow rate of the aeration device is 100L / min, the anode is an aluminum plate, the distance between the cathode and the anode is 90mm, the saturated calomel electrode is used as the reference electrode, and the DC stabilized current power supply controls the cathode potential to - 1.2V. The bisphenol A solution (100 mg / L concentration) enters the enzyme-electrode coupled electrocoagulation reactor from the water inlet, and the residence time is 5 hours. The removal rate of phenol in the effluent treated by the system is 99%. Under the same conditions, the removal rate of bisphenol A was only 35% using the activated carbon electrode without enzyme immobilization. It shows that the synergistic effect of...

Embodiment 3

[0040] The effective volume of the cathode and anode chambers of the reactor is 40mL. A self-made enzyme-modified electrode was used as the cathode, and polyphenol oxidase was selected as the enzyme, and the amount of enzyme added was 10U / cm 2 , the substrate electrode is graphite felt, the air flow rate of the aeration device is 0.1L / min, the anode is an iron plate, the distance between the cathode and the anode is 1mm, the saturated calomel electrode is used as the reference electrode, and the DC stabilized current power supply controls the anode The potential is 0.2V. The humic acid solution (TOC is 100mg / L) enters the enzyme electrode coupled electrocoagulation reactor from the water inlet, the residence time is 1h, and the TOC removal rate of the system treated effluent is 80%. Under the same conditions, the removal rate of TOC was only 15% using graphite felt electrodes without enzyme immobilization; under the same conditions, the removal rate of TOC was 50% only throug...

technical field

[0001] The invention relates to a method for removing organic matter in water. Specifically, the invention proposes a method and a reactor for jointly removing organic matter in water by combining a peroxidase modified electrode and electrocoagulation in the same electrochemical system. Background technique

[0002] With the rapid advancement of my country's industrialization, the effluent of industrial wastewater still contains a large amount of low-concentration dissolved organic matter with various types and complex structures. For example, a certain wastewater is directly discharged after biochemical treatment, but it still contains polyphenols, polycyclic aromatic hydrocarbons, heterocyclic compounds, Low-concentration organic matter such as macromolecule humus. As a result, organic micro-pollution of water bodies in my country has become increasingly serious in recent years and has become an important hidden danger to water body safety.

[0003] Enzyme ...

Examples

Embodiment 1

[0036] The effective volume of the cathode and anode chambers of the reactor is 40mL. A self-made enzyme-modified electrode was used as the cathode, horseradish peroxidase was used as the enzyme, and the amount of enzyme added was 10U / cm 2 , the substrate electrode is graphite, the air flow rate of the aeration device is 0.1L / min, the anode is an aluminum plate, the distance between the cathode and the anode is 1mm, the saturated calomel electrode is used as the reference electrode, and the DC stabilized current power supply controls the cathode potential as -0.2V. The bisphenol A solution (concentration 100mg / L) enters the enzyme electrode coupled electrocoagulation reactor from the water inlet, and the residence time is 5 minutes. The removal rate of bisphenol A in the effluent treated by the system is 90%. Under the same conditions, the removal rate of bisphenol A was only 25% using the graphite electrode without enzyme immobilization. It shows that the synergistic effect...

Embodiment 2

[0038] The effective volume of the cathode and anode compartments of the reactor is 4L. A self-made enzyme-modified electrode is used as the cathode, the enzyme is lignin peroxidase, and the amount of enzyme added is 1000U / cm 2 , the matrix electrode is activated carbon, the air flow rate of the aeration device is 100L / min, the anode is an aluminum plate, the distance between the cathode and the anode is 90mm, the saturated calomel electrode is used as the reference electrode, and the DC stabilized current power supply controls the cathode potential to - 1.2V. The bisphenol A solution (100 mg / L concentration) enters the enzyme-electrode coupled electrocoagulation reactor from the water inlet, and the residence time is 5 hours. The removal rate of phenol in the effluent treated by the system is 99%. Under the same conditions, the removal rate of bisphenol A was only 35% using the activated carbon electrode without enzyme immobilization. It shows that the synergistic effect of...

Embodiment 3

[0040] The effective volume of the cathode and anode chambers of the reactor is 40mL. A self-made enzyme-modified electrode was used as the cathode, and polyphenol oxidase was selected as the enzyme, and the amount of enzyme added was 10U / cm 2 , the substrate electrode is graphite felt, the air flow rate of the aeration device is 0.1L / min, the anode is an iron plate, the distance between the cathode and the anode is 1mm, the saturated calomel electrode is used as the reference electrode, and the DC stabilized current power supply controls the anode The potential is 0.2V. The humic acid solution (TOC is 100mg / L) enters the enzyme electrode coupled electrocoagulation reactor from the water inlet, the residence time is 1h, and the TOC removal rate of the system treated effluent is 80%. Under the same conditions, the removal rate of TOC was only 15% using graphite felt electrodes without enzyme immobilization; under the same conditions, the removal rate of TOC was 50% only throug...

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