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Enzyme electrode

a technology of enzyme electrodes and electrodes, applied in the field of enzyme electrodes, can solve the problems of low reproducibility (not stabilized), inability to carry out stable operation, and limited operation conditions of enzyme electrodes

Active Publication Date: 2010-07-29
ARKRAY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0015]Since the enzyme electrode according to the present invention includes the high-resistance particle enhancing the electrical resistance, the high-resistance particle being chemically stable, the enzyme electrode can stably and reproducibly carry out the electrochemical measurements such as the voltammetric measurement and the amperometric measurement.

Problems solved by technology

However, when voltammetric measurement or amperometric measurement was carried out using the GDH platinized carbon enzyme electrode as an anode electrode, and using glucose of an enzyme ground substance as a measuring object, accordingly, the output characteristics of a measurement sensor including the GDH platinized carbon enzyme electrode were different and the reproducibility was low (not stabilized) whenever a measurement sensor was experimentally produced.
Therefore, in a measurement using a voltammetric method or an amperometric method when carrying out glucose measurement using the GDH platinized carbon enzyme electrode, the stable operation may not be able to be carried out as it is, or the operation condition as the enzyme electrode may be mostly limited.
As a result, the condition range of the appropriate operation may become very narrow.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0056]In this example, the influence of the blending amount of the high-resistance particle to the resistance value of the enzyme electrode will be examined.

(Production of Enzyme Electrode)

[0057]In the production of the enzyme electrode, first, aluminum oxide (“AEROSIL”; manufactured by Degussa AG) and platinized carbon (“IFPC40-II”; manufactured by ISHIFUKU Metal Industry Co., Ltd.) were extracted so that the addition rates thereof are respectively changed and the total amount became 60 mg and were fully mixed to produce a mixed powder. Next, 1000 μL of a GDH solution of 1250 U / mL was added into the mixed powder, and they were mixed to prepare a mixed solution. The mixed solution was left at rest for 6 hours to preliminarily adsorb GDH on platinized carbon, and centrifuged to remove a supernatant fluid. A slurry after the supernatant fluid was removed was powdered by vacuum drying, and 100 μL of liquid paraffin was added into the powder. They were mixed well to produce a paste. Thi...

example 2

[0059]In the example, electric generating capacity as a glucose fuel cell was evaluated for a GDH platinized carbon enzyme electrode.

[0060]The electric generating capacity was evaluated by immersing an enzyme electrode as an anode electrode, and a platinum electrode as a cathode electrode into an electrolyte solution to form a battery. The electric generating capacity was measured in an air release atmosphere with a temperature of the battery set to 25° C.

[0061]Four kinds (one kind is aluminum oxide free) of enzyme electrodes having a different blending ratio of platinized carbon and aluminum oxide were produced as in example 1. The enzyme electrode has a diameter of 3 φ and a thickness of 2 mm. As the platinum electrode, a wire platinum electrode having a diameter of 1 φ and a length of 5 cm was used.

[0062]An electrolyte capacity was set to 10 mL using a phosphoric acid buffer (pH: 6.0) of 100 mmol / L as the electrolyte solution. Glucose was dissolved in the electrolyte solution so ...

example 3

[0066]In this example, the dependency of the glucose concentration of the measured current value for each of the enzyme electrode into which aluminum oxide was added and the enzyme electrode into which aluminum oxide was not added was evaluated.

[0067]The glucose concentration was adjusted by calculating so that the glucose concentration became the aimed concentration to the electrolyte solution used in example 2, and dissolving glucose. The aimed concentration was set to 0 mg / dL, 50 mg / dL, 100 mg / dL, 200 mg / dL, 400 mg / dL, 600 mg / dL and 800 mg / dL.

[0068]A current value was measured as a response value when applying voltage between the enzyme electrode and the platinum electrode by a linear sweep voltammetrical method.

[0069]Three kinds of enzyme electrodes (one kind is aluminum oxide free) having a different blending ratio of the platinized carbon and aluminum oxide were produced as in example 1. Both the enzyme electrode and the platinum electrode had a diameter of 3 φ and a thickness...

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Abstract

The present invention relates to an enzyme electrode including: a carbon particle; a metal particle held on the carbon particle, the metal particle having a catalytic activity against a redox reaction; a redox enzyme. The enzyme electrode of the present invention further includes a high-resistance particle enhancing an electrical resistance, the high-resistance particle being chemically stable. The high-resistance particle contains an inorganic substance, for example. The inorganic substance is aluminum oxide or smectite, for example.

Description

TECHNICAL FIELD[0001]The present invention relates to an enzyme electrode containing a carbon particle holding a metal particle such as a noble metal having a catalytic activity, and a redox enzyme.BACKGROUND ART[0002]Some enzyme electrodes can obtain a response correlated with the concentration of a ground substance in the manner of current measurement using the redox enzyme. An example thereof is obtained by fixing the redox enzyme to platinized carbon (for example, see Patent Document 1 to 3). The enzyme electrode using the platinized carbon advantageously eliminates the need for an electron mediator.[0003]Enzyme electrodes described in Patent Document 1 to 3 are obtained by immobilizing a redox enzyme to a porous layer formed by bonding a platinized carbon particle using a binder. The platinized carbon particle is obtained by holding platinum on the surface of the carbon particle. Platinum is held on the carbon particle as a metal particle, a platinum oxide particle or an oxide ...

Claims

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Application Information

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IPC IPC(8): G01N27/26
CPCC12Q1/001
Inventor KATSUKI, KOJIHIRAO, KONOMUNAGAKAWA, KENJIOKAMOTO, MASASHIFUJINAWA, YOSHIAKI
Owner ARKRAY INC
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