Enterotoxin bioelectrode and its preparation method

A bioelectrode and enterotoxin technology, applied in the field of bioelectrochemical sensors, can solve the problems of cumbersome radioactive pollution, large investment, high price, etc., and achieve the effect of firm rough surface area and increased surface area

Inactive Publication Date: 2005-06-22
725TH RES INST OF CHINA SHIPBUILDING INDAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The existing Staphylococcus aureus enterotoxin (SEC for short) 1 ) detection technology mostly uses optical fiber, piezoelectric quartz crystal, acoustics, microwave and other means, these methods are time-consuming, laborious and expensive
Determination of SEC by radioimmunoassay commonly used in medical research 1 , is also very cumbersome and has serious radioactive pollution, large investment and high cost

Method used

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  • Enterotoxin bioelectrode and its preparation method
  • Enterotoxin bioelectrode and its preparation method
  • Enterotoxin bioelectrode and its preparation method

Examples

Experimental program
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Effect test

example 1

[0032] Example 1: After cross-linking 30U, 20U, 15U, and 10U of glucose oxidase on a platinum-plated electrode, use 0.8mg / ml of SEC 1 Antibodies made in SEC 1 Enzyme immunoelectrode, and detect different SEC 1 The electrode response curve of the antigen concentration is obtained as figure 2 Response characteristics shown. From figure 2 It can be seen from the figure that the electrode sensitivity of cross-linked 30U glucose oxidase is higher, this is because the amount of enzyme is more, the amount of coupled antibody is also more, the probability of antibody and antigen binding is greater during detection, and the enzyme activity released is also more , that is, the generated response current is high, that is, the sensitivity is high. On the contrary, the amount of antibody coupled to the electrode of cross-linked 20U glucose oxidase is less, the probability of binding to the antigen is lower, and the released enzyme activity is also less. Only when the antigen concent...

example 2

[0033] Example 2: Coupling 0.8mg / ml and 3mg / ml of enterotoxin antibody to the enzyme electrode of cross-linked 30U glucose oxidase, and then measuring its effect on different concentrations of SEC 1 Antigen response curve, obtained as image 3 The response characteristic graph shown. From image 3 It can be seen that the detection range of the two electrodes is similar, but the SEC coupled with 0.8mg / ml 1 Antibody-made SEC 1 The enzyme immunoelectrode has high sensitivity. The closer detection range is due to the same amount of enzyme cross-linked by both electrodes. Therefore, no matter how much the antibody concentration differs, the amount of antibody coupled to the electrode is similar, so the maximum amount of antigen that can bind to the antibody is relatively close. The high sensitivity of the electrode coupled with 0.8mg / ml antibody is due to the high concentration (3mg / ml) of antibody inhibits the activity of the enzyme during the process of coupling the antibody...

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Abstract

The invention discloses an enterotoxin bioelectrode, which comprises: a conductive rod-shaped substrate, a platinum black plating layer electroplated on the outer surface of the rod end of the substrate, and an oxidase film fixed on the platinum black plating layer. On the oxidase membrane, an enterotoxin antibody membrane is coupled with glutaraldehyde, and the matrix, platinum black coating, oxidase membrane, and enterotoxin antibody membrane constitute a device for specifically detecting the content of enterotoxin antigen in the sample solution. Enterotoxin enzyme immunoelectrode. The preparation steps are as follows: (1) matrix pretreatment; (2) preparation of platinum black electrode; (3) preparation of enzyme electrode; (4) preparation of enterotoxin enzyme immune electrode. The order of magnitude of the electrode used to detect the sample concentration is ug / ml. It has both the catalytic amplification effect of the enzyme electrode and the specificity of the molecular recognition reaction between the antigen and the antibody of the immune electrode. It has biological activity, high sensitivity and stability, and has the advantages of simple sample preparation, small size, and portability. The test time is short, the response speed is fast, the detection sensitivity is high, and the characteristics are easy to control.

Description

(1) Technical field [0001] The invention relates to the improvement of biotoxin detection technology, specifically an enterotoxin bioelectrode and a preparation method thereof, belonging to the technical field of bioelectrochemical sensors. (2) Background technology [0002] Staphylococcus aureus is a pathogenic bacterium of humans and some animals, and it is the main causative agent of food poisoning. Among them, food poisoning caused by Staphylococcus aureus enterotoxin accounts for 50% of the total bacterial food poisoning. Staphylococcus aureus enterotoxin can contaminate a variety of foods, such as milk, sauced meat, rice, fish, vermicelli and boiled eggs. The existing Staphylococcus aureus enterotoxin (SEC for short) 1 ) Detection technology mostly uses optical fiber, piezoelectric quartz crystal, acoustics, microwave and other means, these methods are time-consuming, laborious and expensive. Determination of SEC by radioimmunoassay commonly used in medical research...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/327
Inventor 董飒英
Owner 725TH RES INST OF CHINA SHIPBUILDING INDAL CORP
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