Nitrogen doped titanium dioxide nanotube array enzyme electrode and preparation method and application thereof

Abstract

The invention provides a glucose oxidase modified nitrogen doped titanium dioxide nanotube array enzyme electrode. The glucose oxidase modified nitrogen doped titanium dioxide nanotube array enzyme electrode comprises glucose oxidase, a nitrogen doped titanium dioxide nanotube array and a titanium substrate, wherein glucose oxidase is immobilized on the surface of a tube opening of the nitrogen doped titanium dioxide nanotube array which is longitudinally arrayed on the titanium substrate; the bottom of each nitrogen doped titanium dioxide nanotube is connected with the surface of the titanium substrate to form an integral structure. The invention also provides a preparation method of the enzyme electrode and application of the enzyme electrode to glucose electrochemical biosensors and glucose bio-enzyme fuel cells. The enzyme electrode is prepared by immobilizing glucose oxidase and a bovine serum albumin-glutaraldehyde crosslinking agent via crosslinking reaction with nitrogen doped titanium dioxide with favorable biocompatibility, hydrophilia and conductivity as an electrode substrate material. The preparation technology is simple, scale production can be realized with easiness in amplification, and the electrode has wide practical application value and prospect.

Description

technical field [0001] The invention belongs to the field of electrochemical materials, and relates to a glucose oxidase-nitrogen-doped titanium dioxide nanotube array enzyme electrode, and also relates to a preparation method of the electrode and an application of an electrochemical biosensor and a bioenzyme fuel cell. Background technique [0002] Titanium dioxide materials have good biocompatibility, excellent hydrophilicity, and low price, and have important applications in the fields of optics, catalysis, and biosensing. At present, a variety of nanostructured titanium dioxide is used in the field of preparation of enzyme electrodes. Although titanium dioxide is a substrate material for enzyme electrodes with good development prospects, as a semiconductor material, its electrical conductivity is poor, which limits its application in the field of bioelectrocatalysis. Therefore, it is very meaningful to explore an effective method that can improve the conductivity of tit...

AI Technical Summary

Problems solved by technology

[0004] Both titanium dioxide and titanium nitride can be used as carrier materials for enzyme immobilization. However, titanium dioxide materials have poor electrical conductivity, titanium nitride has poor hydrophilicity, and the intermediate state of titanium dioxide and titanium nitride—nitrogen-doped titanium dioxide nanomaterials simultaneously With the advantages of these two materials, it not only has good biocompatibility, good hydrophilicity, but also has good conductivity, and can be used as an ideal carrier material for enzyme immobilization

[0005] The traditional cross-linking enzyme immobilization process usually directly mixes bovine serum albumin, glutaraldehyde and glucose oxidase to obtain a cross-linking reaction mixture, and then smears the cross-linking reaction mixture on the surface of the carrier material to achieve enzyme immobilization This often causes the enzyme to embed the active site of the enzyme when it is immobilized, thereby reducing the enzyme activity of the prepared enzyme electrode

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