Nitrogen doped titanium dioxide nanotube array enzyme electrode and preparation method and application thereof

A nanotube array, titanium dioxide technology, applied in the field of electrochemical materials, can solve the problems of poor electrical conductivity, poor hydrophilicity, reduced enzyme activity of enzyme electrodes, etc., and achieve the effects of good hydrophilicity, good conductivity and simple preparation process

Inactive Publication Date: 2014-10-01
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Both titanium dioxide and titanium nitride can be used as carrier materials for enzyme immobilization. However, titanium dioxide materials have poor electrical conductivity, titanium nitride has poor hydrophilicity, and the intermediate state of titanium dioxide and titanium nitride—nitrogen-doped titanium dioxide nanomaterials simultaneously With the advantages of these two materials, it not only has good biocompatibility, good hydrophilicity, but also has good conductivity, and can be used as an ideal

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  • Nitrogen doped titanium dioxide nanotube array enzyme electrode and preparation method and application thereof
  • Nitrogen doped titanium dioxide nanotube array enzyme electrode and preparation method and application thereof
  • Nitrogen doped titanium dioxide nanotube array enzyme electrode and preparation method and application thereof

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Embodiment 1

[0038] The preparation process of glucose oxidase-nitrogen-doped titanium dioxide nanotube array enzyme electrode, see figure 2 , including the following steps:

[0039] (1) Synthesis of titanium dioxide nanotube arrays by constant voltage anodic oxidation method: In a two-electrode electrochemical reaction system, titanium sheets are used as anodes, platinum sheets are used as cathodes, and a mixture of ethylene glycol, water, and ammonium fluoride is used for electrolysis. solution, anodic oxidation reaction was carried out to prepare titanium dioxide nanotube arrays; the conditions of the constant voltage anodic oxidation method were as follows: the oxidation voltage was 60V, the oxidation time was 3h, the reaction temperature was 25°C, and the reaction electrolyte contained water and ammonium fluoride Ethylene glycol organic solution, the mass fraction of water is 0.9%, and the mass fraction of ammonium fluoride is 0.25%.

[0040](2) High-temperature calcination treatmen...

Embodiment 2

[0045] The preparation process of glucose oxidase-nitrogen-doped titanium dioxide nanotube array enzyme electrode, see figure 2 , including the following steps:

[0046] (1) Synthesis of titanium dioxide nanotube arrays by constant voltage anodic oxidation method: In a two-electrode electrochemical reaction system, titanium sheets are used as anodes, platinum sheets are used as cathodes, and a mixture of ethylene glycol, water, and ammonium fluoride is used for electrolysis. solution, anodic oxidation reaction was carried out to prepare titanium dioxide nanotube arrays; the conditions of the constant voltage anodic oxidation method were as follows: the oxidation voltage was 60V, the oxidation time was 2h, the reaction temperature was 23°C, and the reaction electrolyte contained water and ammonium fluoride Ethylene glycol organic solution, the mass fraction of water is 1.8%, and the mass fraction of ammonium fluoride is 0.2%.

[0047] (2) High-temperature calcination treatmen...

Embodiment 3

[0051] The preparation process of glucose oxidase-nitrogen-doped titanium dioxide nanotube array enzyme electrode, see figure 2 , including the following steps:

[0052] (1) Synthesis of titanium dioxide nanotube arrays by constant voltage anodic oxidation method: In a two-electrode electrochemical reaction system, titanium sheets are used as anodes, platinum sheets are used as cathodes, and a mixture of ethylene glycol, water, and ammonium fluoride is used for electrolysis. solution, anodic oxidation reaction was carried out to prepare titanium dioxide nanotube arrays; the conditions of the constant voltage anodic oxidation method were as follows: the oxidation voltage was 60V, the oxidation time was 1h, the reaction temperature was 27°C, and the reaction electrolyte contained water and ammonium fluoride Ethylene glycol organic solution, the mass fraction of water is 2.7%, and the mass fraction of ammonium fluoride is 0.30%.

[0053] (2) High-temperature calcination treatme...

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Abstract

The invention provides a glucose oxidase modified nitrogen doped titanium dioxide nanotube array enzyme electrode. The glucose oxidase modified nitrogen doped titanium dioxide nanotube array enzyme electrode comprises glucose oxidase, a nitrogen doped titanium dioxide nanotube array and a titanium substrate, wherein glucose oxidase is immobilized on the surface of a tube opening of the nitrogen doped titanium dioxide nanotube array which is longitudinally arrayed on the titanium substrate; the bottom of each nitrogen doped titanium dioxide nanotube is connected with the surface of the titanium substrate to form an integral structure. The invention also provides a preparation method of the enzyme electrode and application of the enzyme electrode to glucose electrochemical biosensors and glucose bio-enzyme fuel cells. The enzyme electrode is prepared by immobilizing glucose oxidase and a bovine serum albumin-glutaraldehyde crosslinking agent via crosslinking reaction with nitrogen doped titanium dioxide with favorable biocompatibility, hydrophilia and conductivity as an electrode substrate material. The preparation technology is simple, scale production can be realized with easiness in amplification, and the electrode has wide practical application value and prospect.

Description

technical field [0001] The invention belongs to the field of electrochemical materials, and relates to a glucose oxidase-nitrogen-doped titanium dioxide nanotube array enzyme electrode, and also relates to a preparation method of the electrode and an application of an electrochemical biosensor and a bioenzyme fuel cell. Background technique [0002] Titanium dioxide materials have good biocompatibility, excellent hydrophilicity, and low price, and have important applications in the fields of optics, catalysis, and biosensing. At present, a variety of nanostructured titanium dioxide is used in the field of preparation of enzyme electrodes. Although titanium dioxide is a substrate material for enzyme electrodes with good development prospects, as a semiconductor material, its electrical conductivity is poor, which limits its application in the field of bioelectrocatalysis. Therefore, it is very meaningful to explore an effective method that can improve the conductivity of tit...

Claims

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Application Information

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IPC IPC(8): G01N27/327H01M4/86B82Y15/00
CPCY02E60/50
Inventor 谢一兵王玮
Owner SOUTHEAST UNIV
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