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Application of nano-enzyme electrode in detecting o-dihydroxybenzene

A catechol and nano-enzyme technology, applied in the field of biosensors, can solve the problems that catechol electrodes have not been reported, and achieve the effects of low cost, high sensitivity and wide selectivity

Pending Publication Date: 2021-07-30
CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no nano-enzyme electrode suitable for detecting catechol has been reported.

Method used

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  • Application of nano-enzyme electrode in detecting o-dihydroxybenzene
  • Application of nano-enzyme electrode in detecting o-dihydroxybenzene
  • Application of nano-enzyme electrode in detecting o-dihydroxybenzene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1PCN222 synthesis

[0042] Get 50mg of TCPP, 150mg of zirconium oxychloride octahydrate and 2.8g of benzoic acid dissolved in 50mL of DMF containing 1% (volume concentration) deionized water, ultrasonic 10min at 25°C to mix it evenly, then transfer to React in an oil bath at 90° C. for 4 hours, centrifuge at 13,000 rpm / min for 30 minutes after the reaction, and wash with DMF for 3 times to obtain the target product PCN222. The reaction mechanism of embodiment 1 is as figure 1 As shown, the color changes as figure 2 shown, where figure 2 a is the green color of the reaction system before the reaction, figure 2 b: The reaction system is purple in color after the reaction.

Embodiment 2

[0044]Polish the glassy carbon (GC) on the suede with 0.35 μm polished aluminum powder for 6 min, then ultrasonically clean the polished GC 3 times with deionized water and absolute ethanol, and place the GC obtained by ultrasonic cleaning in 1 molL -1 In the sulfuric acid solution, using cyclic voltammetry, the potential is 0-1.7V, with 0.1Vs -1 The scanning speed was scanned 30 times, ultrasonically cleaned and dried to obtain the GC electrode substrate. The PCN222 obtained in Example 1 is ultrasonically dissolved in water to obtain a concentration of 50mgL -1 PCN222 solution, dissolving chitosan in water to obtain a chitosan solution with a mass concentration of 1%, mixing the obtained PCN222 solution with the chitosan solution at a volume ratio of 1:1 to obtain a mixed solution of PCN222 and chitosan, Take 6 μL of the mixed solution of PCN222 and chitosan and drop it on the GC electrode substrate and let it dry naturally at room temperature to obtain the chitosan-PCN222 / G...

Embodiment 3

[0046] The preparation method is basically the same as in Example 2, the only difference is that the concentration of the PCN222 solution is 1gL -1 .

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Abstract

The invention discloses an application of a nano enzyme electrode in detecting o-dihydroxybenzene. An electrode matrix of the nano-enzyme electrode is modified with a metal organic framework material, and a framework of the metal organic framework material contains a porphyrin ring. The nano-enzyme electrode realizes the detection of o-dihydroxybenzene in a solution to be detected, has high sensitivity and selectivity and a wide linear range, and is simple to manufacture and low in cost.

Description

technical field [0001] The application relates to a nano-enzyme electrode, a biosensor and its preparation method and application, belonging to the technical field of biosensors. Background technique [0002] Ortho-phenol is an important class of phenolic compounds, widely used in the synthesis of chemical materials, but also a substance that is harmful to the human body and the environment, so it is very important to realize its rapid and effective detection. Traditional detection of catechol mainly includes high performance liquid chromatography, fluorescence method, spectrophotometry and capillary electrophoresis, but these methods generally have the disadvantages of time-consuming, expensive equipment, cumbersome operation and so on. Compared with these methods, the electrochemical sensor method has attracted more and more attention due to its advantages of fast response, low cost, simple operation, fast analysis speed, high sensitivity and good selectivity. [0003] Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48
CPCG01N27/30G01N27/48
Inventor 吴立冬徐志远孟庆一肖雨诗曹强刘欢
Owner CHINESE ACAD OF FISHERY SCI
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