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Cationic dipeptide and gold hybridized microsphere and preparation method and application thereof

A cation and gold hybridization technology, applied in the field of materials, can solve the problems of limiting the detection performance of biosensors and weak conductivity of FF-based materials, achieve high sensitivity and fast response performance, improve conductivity, and improve performance.

Inactive Publication Date: 2019-04-09
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although some progress has been made, the weak conductivity of FF-based materials also limits the detection performance of the constructed biosensors.

Method used

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  • Cationic dipeptide and gold hybridized microsphere and preparation method and application thereof
  • Cationic dipeptide and gold hybridized microsphere and preparation method and application thereof
  • Cationic dipeptide and gold hybridized microsphere and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0037] Embodiment 1, preparation cationic dipeptide nano-gold hybrid microsphere and its application

[0038] 1) Preparation of FF-cationic dipeptide (CDP) and gold nanoparticles hybrid microspheres (CDP-AuNPs):

[0039] Weigh the freeze-dried powder of CDP, dissolve CDP with hexafluoroisopropanol (HFIP) as solvent, shake it evenly after standing for a while, so that it is a colorless and transparent solution, and the concentration of CDP is 100mg / mL;

[0040] Draw a certain amount of this solution with a pipette gun and add it to a certain volume of 0.5mmol / L chloroauric acid solution, dilute CDP so that its concentration is 2mg / mL;

[0041] After magnetic stirring for 30 min, it was heated at 60° C. for 2 h in the dark and then taken out to obtain a light purple suspension, which was the suspension of CDP-AuNPs hybrid microspheres.

[0042] 2) Preparation of modified electrodes:

[0043] Pretreatment of GCE: use 1.0, 0.3, 0.05 μm alumina powder to polish the electrode on t...

Embodiment 2

[0054] Embodiment 2, preparation and application of cationic dipeptide nano-gold hybrid microspheres

[0055] 1) Preparation of FF-cationic dipeptide (CDP) and gold nanoparticles hybrid microspheres (CDP-AuNPs):

[0056] Weigh the freeze-dried powder of CDP, dissolve the CDP with hexafluoroisopropanol (HFIP) as a solvent, shake it evenly after standing for a while, so that it is a colorless and transparent solution, and the concentration of CDP is 100mg / mL;

[0057] Draw a certain amount of this solution with a pipette gun and add it to a certain volume of 0.5mmol / L chloroauric acid solution, dilute CDP so that its concentration is 4mg / mL;

[0058] After magnetic stirring for 30 min, it was heated at 60° C. for 4 h in the dark, and then taken out to obtain a light purple suspension, which was the suspension of CDP-AuNPs hybrid microspheres.

[0059] 2) Preparation of modified electrodes:

[0060] Pretreatment of GCE: use 1.0, 0.3, 0.05 μm alumina powder to polish the electro...

Embodiment 3

[0069] Embodiment 3, preparation and application of cationic dipeptide nano-gold hybrid microspheres

[0070] 1) Preparation of FF-cationic dipeptide (CDP) and gold nanoparticles hybrid microspheres (CDP-AuNPs):

[0071] Weigh the freeze-dried powder of CDP, dissolve the CDP with hexafluoroisopropanol (HFIP) as a solvent, shake it evenly after standing for a while, so that it is a colorless and transparent solution, and the concentration of CDP is 100mg / mL;

[0072] Draw a certain amount of this solution with a pipette gun and add it to a certain volume of 0.5mmol / L chloroauric acid solution, dilute CDP so that its concentration is 1mg / mL;

[0073] After magnetic stirring for 30 min, it was heated at 60° C. for 3 h in the dark and then taken out to obtain a light purple suspension, which was the suspension of CDP-AuNPs hybrid microspheres.

[0074] 2) Preparation of modified electrodes:

[0075] Pretreatment of GCE: use 1.0, 0.3, 0.05 μm alumina powder to polish the electrod...

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Abstract

The invention discloses a cationic dipeptide and gold hybridized microsphere and a preparation method and application thereof. The microsphere is prepared by biocompatible peptide biomolecules and chloroauric acid through reaction under conditions of stirring and heating. The preparation method comprises the following steps of: dissolving the peptide through a solvent; adding the dissolved peptideinto water solution of the chloroauric acid; stirring the mixture for a certain time; and heating the mixture again to form the microsphere. The preparation method of the microsphere has the characteristics of being simple and easy to do, mild in condition and good in repeatability; the microsphere has good stability and biocompatibility; the doping of gold in the prepared microsphere is capableof improving the conductivity of the microsphere and catalytic performance of the microsphere relative to biological detection application, and effectively improving the performance of electrochemicalsensors; the cationic dipeptide and gold hybridized microsphere prepared by the method can be applied to biological sensing analysis; and an obtained enzyme electrode can be used for cholesterol detection, and has relatively high sensitivity and rapid response performance.

Description

technical field [0001] The invention belongs to the application of materials, and relates to a cationic dipeptide and gold hybrid microsphere and its preparation method and application. Background technique [0002] Constructing micro-nano structures with biological primitives for sensing is one of the current research hotspots, and the self-assembly properties of biomolecules provide a novel and convenient method for constructing structures with various morphologies. Peptides have broad application prospects in electrochemical sensors because of their molecular recognition ability, biocompatibility and easy modification characteristics. Among the many peptide-based building blocks, the phenylalanine dipeptide (FF) molecule and its derivatives are the simplest, and their self-assembly can form various micro-nano structures such as nanotubes, nanowires, vesicles, and nanospheres. ; FF nanotubes have the advantages of large specific surface area, high electron transport effic...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3271
Inventor 李峻柏王克青王晨蕾崔巍许有前
Owner INST OF CHEM CHINESE ACAD OF SCI
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